Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

microRNAs (miRNAs), a class of endogenous non‐coding RNAs, are involved in many crucial biological processes, which have emerged as a new set of biomarkers for disease theranostics. Exploring efficient signal amplification strategy is highly desired to pursue an ultrasensitive miRNA biosensing platform. In this work, we have developed a novel electrochemical approach for the analysis of trace miRNA. Target triggered DNA assembly is performed on the surface of the electrode. Meanwhile, gold‐nanoparticle tags are integrated on the product of hybridization chain reaction, which further enhance electrochemical response towards initiator miRNA. The as‐prepared electrochemical biosensor shows good analytical performances and satisfactory practical utility. It has great potential to provide a powerful tool for the miRNA assay in biomedical fields.

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“…Masud et al. prepared nanostructured mesoporous gold electrode for miRNA assay with attomolar sensitivity [18] …”

Section: Methodsmentioning

confidence: 99%

Trace miRNA Assay Based on DNA Nanostructures Formed by Hybridization Chain Reaction and Gold‐Nanoparticle Tags

Song

Wang²,

Qian

et al. 2021

ChemElectroChem

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“…This method avoids cumbersome PCR and enzymatic amplification steps. This is a single-step assay capable of detecting a wide dynamic linear range of 100 aM to 1 nM with an ultra-low limit detection (100 aM) of miRNAs [93]. Jou et al designed an isothermal, dual signal amplification strategy to translate a biosensing event for the pancreatic cancer biomarker miR-196b into electrochemical signals.…”

Section: Dna and Rna Sensorsmentioning

confidence: 99%

Technological advances in electrochemical biosensors for the detection of disease biomarkers

et al. 2021

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With an increasing focus on health in contemporary society, interest in the diagnosis, treatment, and prevention of diseases has grown rapidly. Accordingly, the demand for biosensors for the early diagnosis of disease is increasing. However, the measurement range of existing electrochemical sensors is relatively high, which is not suitable for early disease diagnosis, requiring the detection of small amounts of biocomponents. Various attempts have been made to overcome this and amplify the signal, including binding with various labeling molecules, such as DNA, enzymes, nanoparticles, and carbon materials. Efforts are also being made to increase the sensitivity of electrochemical sensors, and the combination of nanomaterials, materials, and biotechnology offers the potential to increase sensitivity in a variety of ways. Recent studies suggest that electrochemical sensors can be a powerful tool in providing comprehensive insights into the targeting and detection of disease-associated biomarkers. Significant advances in nanomaterial and biomolecule approaches for improved sensitivity have resulted in the development of electrochemical biosensors capable of detecting multiple biomarkers in real time in clinically relevant samples. In this review, we have discussed the recent studies on electrochemical sensors for detection of diseases such as diabetes, degenerative diseases, and cancer. Further, we have highlighted new technologies to improve sensitivity using various materials, including DNA, enzymes, nanoparticles, and carbon materials.

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“…[8][9][10][11][12][13] Meanwhile, electrochemical biosensors by modifying carbon nanotubes (CNTs, including single-walled CNTs and Multi-walled CNTs) or the other 1D-nanomaterials could improve the analytical performance for detecting miR-21 in sensitivity, selectivity, rapidity, and accuracy, due to high specific surface area. [14][15][16][17][18][19][20] Currently, CNTs were incorporated into the polymer to form modification materials for electrochemicalsignal amplification in CNT-modified biosensors. [21,22] However, CNTs were mainly randomly coated onto the surface of the electrode.…”

Section: Introductionmentioning

confidence: 99%

“…[ 8–13 ] Meanwhile, electrochemical biosensors by modifying carbon nanotubes (CNTs, including single‐walled CNTs and Multi‐walled CNTs) or the other 1D‐nanomaterials could improve the analytical performance for detecting miR‐21 in sensitivity, selectivity, rapidity, and accuracy, due to high specific surface area. [ 14–20 ]…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

Wang

et al. 2022

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Abnormal expression of microRNA‐21 (miR‐21) is considered to be closely associated with the pathogenesis of colorectal cancer. However, great challenges do exist for the development of ultra‐sensitive biosensors to detect the abnormal expression of miR‐21 due to the low concentration in serum (fm level) at the early stage of colorectal cancer. Therefore, electric field force is used to rotate and rearrange random multi‐walled carbon nanotubes (MWCNTs) at the microscale to improve the active sites of the electrode in this study. The free‐standing MWCNTs are densely and high‐orderly embedded into the bare electrode along the direction of the electric field. Compared to the bare electrode, the peak‐current response of the free‐standing MWCNT electrode improves by 150 times in cyclic voltammetric measurement. A nano‐genosensor based on the free‐standing MWCNT electrode is developed for measuring miR‐21. The nano‐genosensor for miR‐21 shows an ultra‐high sensitivity of 48.24 µA µm−1, a wide linear range from 0.01 × 10−15 to 100 × 10−12 m, and a low detection limit of 1.2 × 10−18 m. The present nano‐genosensor shows superior performance for miR‐21 in human serum samples and demonstrates a potential application for the diagnosis of early stage colorectal cancer.

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Nanostructured mesoporous gold biosensor for microRNA detection at attomolar level

Cited by 63 publications

References 42 publications

Trace miRNA Assay Based on DNA Nanostructures Formed by Hybridization Chain Reaction and Gold‐Nanoparticle Tags

Trace miRNA Assay Based on DNA Nanostructures Formed by Hybridization Chain Reaction and Gold‐Nanoparticle Tags

Technological advances in electrochemical biosensors for the detection of disease biomarkers

Fabrication of a Free‐Standing MWCNT Electrode by Electric Field Force for an Ultra‐Sensitive MicroRNA‐21 Nano‐Genosensor

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