Nanostructured poly (lactic acid) electrospun fiber with high loadings of TiO2 nanoparticles: Insights into bactericidal activity and cell viability

Toniatto, T V; Rodrigues, Bruno V. M.; Marsi, Teresa C.O.; Ricci, Ritchelli; Marciano, Fernanda R.; Webster, Thomas J.; Lobo, Anderson Oliveira

doi:10.1016/j.msec.2016.10.026

Cited by 58 publications

(34 citation statements)

References 37 publications

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“…In addition, the particle size, estimated from the selected area electron diffraction pattern, was found to be 10-20 nm, which was verified by light transmittance and opacity data. The similar result was reported by Toniatto et al who discovered that through the electrospinning progress, a homogeneous distribution of nano-TiO 2 enabled with PLA was formed [8].…”

Section: Morphological Characteristics Of Pla/tio 2 Nanofibers and Plsupporting

confidence: 86%

“…It is well known that TiO 2 and various TiO 2 -based photocatalysts can only become active under light irradiation. Toniatto et al reported that the bactericidal effect of electrospinning PLA/TiO 2 nanofibers against S. aureus was strong after UV irradiation, which activated the TiO 2 nanoparticles, and that the effect was influenced by the time associated to TiO 2 concentration [8]. Fonseca et al measured the antimicrobial effect of PLA/TiO 2 nanocomposite films including 8 wt.% nano-TiO 2 under UV irradiation and reported the killing effect on bacteria and fungi achieved 94.3% and 99.9%, respectively [5].…”

Section: Antibacterial Propertymentioning

confidence: 99%

“…Toniatto et al developed nanostructured PLA/TiO 2 nanofibers by electrospinning. The nanostructured PLA/TiO 2 nanofibers presented no mammalian cell toxicity and, most importantly, possessed bactericidal activity with larger TiO 2 loadings [8]. Xing et al investigated the effects on the antibacterial and physical properties of polyethylene (PE)-based films after being loaded with nano-TiO 2 particles; their conclusions indicate that the TiO 2 nanoparticles play the important role in antibacterial activity of PE/TiO 2 films [9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Physico-Mechanical and Antibacterial Properties of PLA/TiO2 Composite Materials Synthesized via Electrospinning and Solution Casting Processes

Zhang

Ahmed

et al. 2019

Coatings

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In this study, PLA/TiO2 composites materials were prepared via electrospinning and solution casting processes. By testing the mechanical properties, water contact angle, water vapor permeability, and solubility of the composite nanofibers and films, the comprehensive performances of the two types of nanocomposites were analyzed. The results show that maximum tensile strengths of 2.71 ± 0.11 MPa and 14.49 ± 0.13 MPa were achieved for the nanofibers and films at a TiO2 content of 0.75 wt.%. Moreover, the addition of TiO2 significantly cut down the water vapor transmittance rate of the nanofibers and films while significantly improving the water solubility. Further, the antibacterial activity increased under UV-A irradiation for a TiO2 nanoparticle content of 0.75 wt.%, and the nanofiber and films exhibited inhibition zones of 4.86 ± 0.50 and 3.69 ± 0.40 mm for E. coli, and 5.98 ± 0.77 and 4.63 ± 0.45 mm for S. aureus, respectively. Overall, the performance of the nanofiber was better than that of the film. Nevertheless, both the nanocomposite membranes satisfied the requirements of food packaging materials.

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Section: Morphological Characteristics Of Pla/tio 2 Nanofibers and Plsupporting

confidence: 86%

Section: Antibacterial Propertymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physico-Mechanical and Antibacterial Properties of PLA/TiO2 Composite Materials Synthesized via Electrospinning and Solution Casting Processes

Zhang

Ahmed

et al. 2019

Coatings

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“…This makes them promising materials for biomedical applications as they have been shown to optimize the tissue repair process which in turn speeds up patient recovery times (Simoes, 2011;Wang et al, 2014;Fonseca et al, 2015). Among the various polymers, poly (lactic acid) (PLA) has proven to be an ideal candidate material due to its mechanical properties, good biocompatibility, low cost, and the adjustable degradation profile with CO 2 , H 2 O (Hidalgo et al, 2013;Tawakkal et al, 2014;Annunziata et al, 2015;Toniatto et al, 2017) and polyester as by-products, either from the esterification of lactic acid and its fermentation (Toniatto et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization

Marsi

Ricci

Toniato

et al. 2019

Front. Bioeng. Biotechnol.

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Poly (lactic acid) (PLA) has been increasingly used in cutaneous tissue engineering due to its low cost, ease of handling, biodegradability, and biocompatibility, as well as its ability to form composites. However, these polymers possess a structure with nanoporous that mimic the cellular environment. In this study, nanocomposites are prepared using PLA and titanium dioxide (TiO 2) (10 and 35%-w/w) nanoparticles that also function as an active anti-scarring agent. The nanocomposites were prepared using an electrospinning technique. Three different solutions were prepared as follows: PLA, 10% PLA/TiO 2 , and 35% PLA/TiO 2 (w/w%). Electrospun PLA and PLA/TiO 2 nanocomposites were characterized morphologically, structurally, and chemically using electron scanning microscopy, transmission electron microscopy, goniometry, and X-ray diffraction. L929 fibroblast cells were used for in vitro tests. The cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Versicam (VCAN), biglicam (BIG), interleukin-6 (IL6), interleukin-10 (IL-10), and type-1 collagen (COL1A1) genes were evaluated by RT-qPCR. In vivo tests using Wistar rats were conducted for up to 15 days. Nanofibrous fibers were obtained for all groups that did not contain residual solvents. No cytotoxic effects were observed for up to 168 h. The genes expressed showed the highest values of versican and collagen-1 (p < 0.05) for PLA/TiO 2 nanocomposite scaffolds when compared to the control group (cells). Histological images showed that PLA at 10 and 35% w/w led to a discrete inflammatory infiltration and expression of many newly formed vessels, indicating increased metabolic activity of this tissue. To summarize, this study supported the potential of PLA/TiO 2 nanocomposites ability to reduce cutaneous scarring in scaffolds.

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“…Although several methods (e.g. bubble electrospinning, bubbfil spinning and centrifugal electrospinning) [2][3][4][5] exist to engineer continuous or medicated (bio-active) polymeric fiber systems on a larger scale; the electrospinning (ES) method is probably the most facile and is operational at ambient conditions making it ideal for sensitive biomolecules or other biologics (e.g.cells and proteins) [6]. Furthermore, such single matrix-based drug delivery systems offer controlled and site-specific delivery of molecules of interest [7].…”

Section: Introductionmentioning

confidence: 99%

Non-concentric multi-compartment fibers fabricated using a modified nozzle in single-step electrospinning

Wang

Chang

et al. 2017

Materials Letters

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Multi-compartment fibers with well-defined regions and functional media hosting potential provide coencapsulation opportunities for a variety of bio-molecules, drugs and even nutrients. In this research, fibers possessing multiple compartments were prepared using the single-step electrospinning method and a modified nonconcentric multi-needle. Polyvinyl pyrrolidone (PVP) was used as the fiber shell material, while ketoconazole (KCZ, model drug) and Sudan Red (model probe) were encapsulated as two separate segments running along the fiber length. Multi-compartment fiber morphology and structure were examined using optical and electron microscopy.The effect of flow rate on fiber morphology was also investigated and the release of encapsulated KCZ and Sudan Red was examined using UV spectroscopy. The results present an efficient and promising method to engineer multicompartment fibers in a single step for several biomedical applications in lieu.

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Nanostructured poly (lactic acid) electrospun fiber with high loadings of TiO2 nanoparticles: Insights into bactericidal activity and cell viability

Cited by 58 publications

References 37 publications

Physico-Mechanical and Antibacterial Properties of PLA/TiO2 Composite Materials Synthesized via Electrospinning and Solution Casting Processes

Physico-Mechanical and Antibacterial Properties of PLA/TiO2 Composite Materials Synthesized via Electrospinning and Solution Casting Processes

Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization

Non-concentric multi-compartment fibers fabricated using a modified nozzle in single-step electrospinning

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