Nanostructured silver fabric as a free-standing NanoZyme for colorimetric detection of glucose in urine

Karim, Md. Nurul; Anderson, Samuel R.; Singh, Sanjay; Ramanathan, Rajesh; Bansal, Vipul

doi:10.1016/j.bios.2018.03.025

Cited by 257 publications

(75 citation statements)

References 78 publications

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“…Although frequent blood-glucose monitoring can bring benefits to the health of diabetic patients [2], the disinfection process and stabbing pain during blood collection lead to very poor compliance. Besides blood glucose, the content of urine glucose is another important index for the clinical diagnosis and routine self-monitoring of the patients [3][4][5]. As urine glucose detection is non-invasive, patients' compliance is good, and more emphasis was placed on urine glucose determination.…”

Section: Introductionmentioning

confidence: 99%

“…Colorimetric analysis is a method to determine the content of a substance to-be-measured by the color of the solution that features good stability, low cost, and implementation simplicity, making it widely applied to clinical diagnosis and detection, including in glucose analysis [3,[6][7][8]. However, current liquid colorimetry primarily requires lab-based infrastructure, and its utility greatly increases the use of point-of-use deployment.…”

Section: Introductionmentioning

confidence: 99%

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Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Wang

Guo

et al. 2020

Chemosensors

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In this work, a label-free colorimetric assay was developed for the determination of urine glucose using smartphone ambient-light sensor (ALS). Using horseradish peroxidase—hydrogen peroxide—3,3′,5,5′-tetramethylbenzidine (HRP-H2O2-TMB) colored system, quantitative H2O2 was added to samples to-be-determined for deepest color. The presence of glucose oxidase in urine led to the formation of H2O2 and the reduction of TMBred. As a result of this, the color of the urine faded and the solution changed from deep blue to light blue. We measured the illuminance of the transmitted light by a smartphone ambient light sensor, and thereby color changes were used to calculate the content of urine glucose. After method validation, this colorimetric assay was practically applied for the determination of urine samples from diabetic patients. Good linearity was obtained in the range of 0.039–10.000 mg/mL (R2 = 0.998), and a limit of detection was 0.005 mg/mL. Our method was had high accuracy, sensitivity, simplicity, rapidity, and visualization, providing a new sensor to be potentially applicable for point-of-care detection of urine glucose.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Wang

Guo

et al. 2020

Chemosensors

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“…The SPR excitation are strongly sensitive to the changes of refractive index on the sensing surface, and therefore the shift in the incident angle (in angular interrogation mode) or the optical wavelength (in wavelength interrogation mode) indicate presence of analyte in the sample [ 18 ]. The SPR technique has received continuous interest in various applications involving hazardous gases [ 19 – 21 ], heavy metal ions [ 22 , 23 ], drug molecules [ 24 ], multi-analytes [ 18 , 25 ], glucose [ 26 ] and immuno-sensing [ 27 ], and biomolecular binding studies [ 28 ] due to its advantages of real-time and label-free sensing technique.…”

Section: Introductionmentioning

confidence: 99%

Urea and creatinine detection on nano-laminated gold thin film using Kretschmann-based surface plasmon resonance biosensor

et al. 2018

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This work investigates the surface plasmon resonance (SPR) response of 50-nm thick nano-laminated gold film using Kretschmann-based biosensing for detection of urea and creatinine in solution of various concentrations (non-enzymatic samples). Comparison was made with the presence of urease and creatininase enzymes in the urea and creatinine solutions (enzymatic samples), respectively. Angular interrogation technique was applied using optical wavelengths of 670 nm and 785 nm. The biosensor detects the presence of urea and creatinine at concentrations ranging from 50–800 mM for urea samples and 10–200 mM for creatinine samples. The purpose of studying the enzymatic sample was mainly to enhance the sensitivity of the sensor towards urea and creatinine in the samples. Upon exposure to 670 nm optical wavelength, the sensitivity of 1.4°/M was detected in non-enzymatic urea samples and 4°/M in non-enzymatic creatinine samples. On the other hand, sensor sensitivity as high as 16.2°/M in urea-urease samples and 10°/M in creatinine-creatininase samples was detected. The enhanced sensitivity possibly attributed to the increase in refractive index of analyte sensing layer due to urea-urease and creatinine-creatininase coupling activity. This work has successfully proved the design and demonstrated a proof-of-concept experiment using a low-cost and easy fabrication of Kretschmann based nano-laminated gold film SPR biosensor for detection of urea and creatinine using urease and creatininase enzymes.

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“…Recent developments in nanotechnology have also aimed towards the construction of novel enzyme mimetics (nanozymes) exhibiting biological oxidase, peroxidase, catalase, and superoxide dismutase-like activities (Gao et al 2017 ; Karim et al 2018 ; Lin et al 2014d ; Shah and Singh 2018 ). Nanozymes are nanomaterials possessing intrinsic biological enzyme-like properties, which offer several advantages over natural enzymes such as high stability and activity at varying conditions of pH and temperatures, cost effectivity, easy manipulation and multiple applications on a single platform.…”

Section: Bio-sensing Applications Of Iron Oxide Nanoparticlesmentioning

confidence: 99%

Recent advances and future prospects of iron oxide nanoparticles in biomedicine and diagnostics

2018

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Superparamagnetic iron oxide nanoparticles (SPIONs) are considered as chemically inert materials and, therefore, being extensively applied in the areas of imaging, targeting, drug delivery and biosensors. Their unique properties such as low toxicity, biocompatibility, potent magnetic and catalytic behavior and superior role in multifunctional modalities have epitomized them as an appropriate candidate for biomedical applications. Recent developments in the area of materials science have enabled the facile synthesis of Iron oxide nanoparticles (IONPs) offering easy tuning of surface properties and surface functionalization with desired biomolecules. Such developments have enabled IONPs to be easily accommodated in nanocomposite platform or devices. Additionally, the tag of biocompatible material has realized their potential in myriad applications of nanomedicines including imaging modalities, sensing, and therapeutics. Further, IONPs enzyme mimetic activity pronounced their role as nanozymes in detecting biomolecules like glucose, and cholesterol etc. Hence, based on their versatile applications in biomedicine, the present review article focusses on the current trends, developments and future prospects of IONPs in MRI, hyperthermia, photothermal therapy, biomolecules detection, chemotherapy, antimicrobial activity and also their role as the multifunctional agent in diagnosis and nanomedicines.

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Nanostructured silver fabric as a free-standing NanoZyme for colorimetric detection of glucose in urine

Cited by 257 publications

References 78 publications

Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Urea and creatinine detection on nano-laminated gold thin film using Kretschmann-based surface plasmon resonance biosensor

Recent advances and future prospects of iron oxide nanoparticles in biomedicine and diagnostics

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