Nanostructured Substrates as Matrices for Surface Assisted Laser Desorption/Ionization Mass Spectrometry: A Progress Report from Material Research to Biomedical Applications

Ma, Wen; Li, Jun; Li, Xianjiang; Bai, Yu; Liu, Huwei

doi:10.1002/smtd.202100762

Cited by 44 publications

(30 citation statements)

References 187 publications

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“…LDI-MS in metabolite detection. [12] Furthermore, the cocrystallization heterogeneity of these conventional matrices and analytes results in poor reproducibility and brings difficulty to the quantitation of analytes by LDI-MS. [13] Surprisingly, given excellent light absorption properties, homogeneous dispersibility, large surface area, and multiple chemical surface modification, many nanomaterials have been selected as matrices of LDI-MS to overcome the above shortcomings. [14][15][16][17] Especially, among the developed matrix nanomaterials, titania can effectively absorb UV light from laser source of LDI-MS to exert photocatalytic properties, thereby facilitating the desorption/ionization of analytes.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Urinary Exosome Metabolic Patterns in Membranous Nephropathy by Titania‐Assisted Intact Exosome Mass Spectrometry

Chen

Zhang

et al. 2022

Small Science

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Exosomes are regarded as the emerging potential targets for liquid biopsy and bioprocess study owing to their abundant inclusive cargos that carry significant disease information. In addition, metabolites have been promising biomarkers for diagnosis. However, little metabolic research on exosomes is carried out by now. Herein, the mix‐crystal titania‐assisted laser desorption/ionization mass spectrometry (LDI‐MS) method is established, which features fast speed, high throughput, and efficiency, to directly extract urinary exosome metabolic patterns of healthy controls (HC) and membrane nephropathy (MN) patients. Besides, this method is also adopted to acquire the primitive urinary metabolic patterns from the same samples for comparison. By taking advantage of principal component analysis, unpaired parametric t‐test, and orthogonal partial least squares discriminant analysis on the exosome metabolic patterns, 27 significant m/z signals are filtrated, which possess more prominent differentiation capacity toward HC and MN patients (AUC = 0.942), and hold greater potential in MN diagnosis, compared to primitive urine (AUC = 0.801). The work reveals the important clinical value of exosome metabolic analysis, and paves a way to exosome‐based diagnosis at metabolomic level toward large‐scale clinical use.

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Section: Introductionmentioning

confidence: 99%

Investigation of Urinary Exosome Metabolic Patterns in Membranous Nephropathy by Titania‐Assisted Intact Exosome Mass Spectrometry

Chen

Zhang

et al. 2022

Small Science

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“…Traditional LM circuit preparation has been challenging to develop further. Laser technology has been widely used in various fields, for example: textile electronic [ 39 ], composite materials [ 40 ], biomedical science [ 41 , 42 , 43 ], and so on. To the best of our knowledge, there have been few reports on the fabrication of ultra-micro LM electronic devices by laser engraving [ 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

Laser-Engraved Liquid Metal Circuit for Wearable Electronics

Liang

Song

2022

Bioengineering

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Conventional patterning methods for producing liquid metal (LM) electronic circuits, such as the template method, use chemical etching, which requires long cycle times, high costs, and multiple-step operations. In this study, a novel and reliable laser engraving micro-fabrication technology was introduced, which was used to fabricate personalized patterns of LM electronic circuits. First, by digitizing the pattern, a laser printing technology was used to burn a polyethylene (PE) film, where a polydimethylsiloxane (PDMS) or paper substrate was used to produce grooves. Then, the grooves were filled with LM and the PE film was removed; finally, the metal was packaged with PDMS film. The experimental results showed that the prepared LM could fabricate precise patterned electronic circuits, such as golden serpentine curves and Peano curves. The minimum width and height of the LM circuit were 253 μm and 200 μm, respectively, whereas the printed LM circuit on paper reached a minimum height of 26 μm. This LM flexible circuit could also be adapted to various sensor devices and was successfully applied to heart rate detection. Laser engraving micro-processing technologies could be used to customize various high-resolution LM circuit patterns in a short time, and have broad prospects in the manufacture of flexible electronic equipment.

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“…Considerable efforts have been devoted to the design and fabrication of nanostructured layers with functional properties by regulating and controlling the surface chemistry, resulting in very promising substrates for protein microarray applications and third-generation point-of-care (POC) biosensors [8][9][10][11][12]. In particular, nanostructured metal oxides allow for the fine tuning of physical and chemical properties, such as surface wettability and amphiphilicity, which have a remarkable role in regulating the interaction between the nanostructured surface and the biological matter [13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS

et al. 2022

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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) on cluster-assembled super-hydrophilic nanoporous titania films deposited on hydrophobic conductive-polymer substrates feature a unique combination of surface properties that significantly improve the possibilities of capturing and processing biological samples before and during the MALDI-MS analysis without changing the selected sample target (multi-dimensional MALDI-MS). In contrast to pure hydrophobic surfaces, such films promote a remarkable biologically active film porosity at the nanoscale due to the soft assembling of ultrafine atomic clusters. This unique combination of nanoscale porosity and super-hydrophilicity provides room for effective sample capturing, while the hydrophilic-hydrophobic discontinuity at the border of the dot-patterned film acts as a wettability-driven containment for sample/reagent droplets. In the present work, we evaluate the performance of such advanced surface engineered reactive containments for their benefit in protein sample processing and characterization. We shortly discuss the advantages resulting from the introduction of the described chips in the MALDI-MS workflow in the healthcare/clinical context and in MALDI-MS bioimaging (MALDI-MSI).

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Nanostructured Substrates as Matrices for Surface Assisted Laser Desorption/Ionization Mass Spectrometry: A Progress Report from Material Research to Biomedical Applications

Abstract: Figure 1. Nanostructured substrates-based LDI-MS and its biomedical applications. Three insets on the right (from top to bottom) are reproduced with permissions.

Cited by 44 publications

References 187 publications

Investigation of Urinary Exosome Metabolic Patterns in Membranous Nephropathy by Titania‐Assisted Intact Exosome Mass Spectrometry

Investigation of Urinary Exosome Metabolic Patterns in Membranous Nephropathy by Titania‐Assisted Intact Exosome Mass Spectrometry

Laser-Engraved Liquid Metal Circuit for Wearable Electronics

Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS

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