Nanotechnology for mesenchymal stem cell therapies

Corradetti, Bruna; Ferrari, Mauro

doi:10.1016/j.jconrel.2015.12.042

Cited by 30 publications

(17 citation statements)

References 144 publications

(118 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…Mesenchymal stem cells (MSC) are promising candidates for cell-based therapy to treat several diseases 1 – 5 . They are compelling to consider as vehicles for delivery of biologically active agents 1 . Although systemic administration of MSC is considered the optimal route for clinical trials 6 , the major challenge is to efficiently deliver them to a target location and enhance engraftment 7 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation

Corradetti

Taraballi

Martinez

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

A major challenge in regenerative medicine is to improve therapeutic cells’ delivery and targeting using an efficient and simple protocol. Mesenchymal stem cells (MSC) are currently employed for the treatment of inflammatory-based diseases, due to their powerful immunosoppressive potential. Here we report a simple and versatile method to transiently overexpress the hyaluronic acid (HA) receptor, CD44, on MSC membranes, to improve their homing potential towards an inflammatory site without affecting their behavior. The effect of HA-coatings on murine MSC was functionally determined both, in vitro and in vivo as a consequence of the transient CD44 overexpression induced by HA. Data obtained from the in vitro migration assay demonstrated a two-fold increase in the migratory potential of HA-treated MSC compared to untreated cells. In an LPS-induced inflamed ear murine model, HA-treated MSC demonstrated a significantly higher inflammatory targeting as observed at 72 hrs as compared to untreated cells. This increased accumulation for HA-treated MSC yielded a substantial reduction in inflammation as demonstrated by the decrease in the expression of pro-inflammatory markers and by the induction of a pro-regenerative environment.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation

Corradetti

Taraballi

Martinez

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Third, the field of mesenchymal stem cell (MSC) transplantation can provide guidance towards determining or increasing the trafficking, survival and tracking of transplanted MΦs [138, 139]. While in depth tracking studies of intravenously injected MΦs have yet to be performed, the results in MSC transplantation mimic some aspects of the results observed in the human clinical trials of MΦ cell therapy.…”

Section: Overall Outlook - Potential Pitfalls and Opportunitiesmentioning

confidence: 99%

“…Pretreatment of transplanted cells has been employed in attempts to reduce lung entrapment and/or increase cell survival [59, 138, 139]. Fischer and colleagues blocked adhesion of MSCs to endothelial cells by inactivation of the VCAM-1 counter-ligand (CD49d) on MSCs and administered the MSCs in two boluses.…”

Section: Overall Outlook - Potential Pitfalls and Opportunitiesmentioning

confidence: 99%

Macrophage-based cell therapies: The long and winding road

Lee

Kivimäe

Dolor

et al. 2016

Journal of Controlled Release

160

143

View full text Add to dashboard Cite

In the quest for better medicines, attention is increasingly turning to cell-based therapies. The rationale is that infused cells can provide a targeted therapy to precisely correct a complex disease phenotype. Between 1987 and 2010, autologous macrophages (MΦs) were used in clinical trials to treat a variety of human tumors; this approach provided a modest therapeutic benefit in some patients but no lasting remissions. These trials were initiated prior to an understanding of: the complexity of MΦ phenotypes, their ability to alter their phenotype in response to various cytokines and/or the environment, and the extent of survival of the re-infused MΦs. It is now known that while inflammatory MΦs can kill tumor cells, the tumor environment is able to reprogram MΦs into a tumorigenic phenotype; inducing blood vessel formation and contributing to a cancer cell growth-promoting milieu. We review how new information enables the development of large numbers of ex vivo generated MΦs, and how conditioning and gene engineering strategies are used to restrict the MΦ to an appropriate phenotype or to enable production of therapeutic proteins. We survey applications in which the MΦ is loaded with nanomedicines, such as liposomes ex vivo, so when the drug-loaded MΦs are infused into an animal, the drug is released at the disease site. Finally, we also review the current status of MΦ biodistribution and survival after transplantation into an animal. The combination of these recent advances opens the way for improved MΦ cell therapies.

show abstract

“…[121] Other polymers such as chitosan and palmitic acid were used for gene transfection in hMSCs. [122] Superparamagnetic iron oxide NPs were used to deliver the brain-derived neurotrophic factor (BDNF) gene in hMSCs. Transfection efficiency was higher compared to polymer (PEI) based delivery and transfected cells were functional in a rat sciatic nerve defect model.…”

Section: Nanotechnology and Stem Cell Manipulationmentioning

confidence: 99%

Engineering Concepts in Stem Cell Research

Narayanan

Mishra

Singh

et al. 2017

Biotechnology Journal

View full text Add to dashboard Cite

The field of regenerative medicine integrates advancements made in stem cells, molecular biology, engineering, and clinical methodologies. Stem cells serve as a fundamental ingredient for therapeutic application in regenerative medicine. Apart from stem cells, engineering concepts have equally contributed to the success of stem cell based applications in improving human health. The purpose of various engineering methodologies is to develop regenerative and preventive medicine to combat various diseases and deformities. Explosion of stem cell discoveries and their implementation in clinical setting warrants new engineering concepts and new biomaterials. Biomaterials, microfluidics, and nanotechnology are the major engineering concepts used for the implementation of stem cells in regenerative medicine. Many of these engineering technologies target the specific niche of the cell for better functional capability. Controlling the niche is the key for various developmental activities leading to organogenesis and tissue homeostasis. Biomimetic understanding not only helped to improve the design of the matrices or scaffolds by incorporating suitable biological and physical components, but also ultimately aided adoption of designs that helped these materials/devices have better function. Adoption of engineering concepts in stem cell research improved overall achievement, however, several important issues such as long-term effects with respect to systems biology needs to be addressed. Here, in this review the authors will highlight some interesting breakthroughs in stem cell biology that use engineering methodologies.

show abstract

Nanotechnology for mesenchymal stem cell therapies

Cited by 30 publications

References 144 publications

Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation

Hyaluronic acid coatings as a simple and efficient approach to improve MSC homing toward the site of inflammation

Macrophage-based cell therapies: The long and winding road

Engineering Concepts in Stem Cell Research

Contact Info

Product

Resources

About