Nanotip analysis for dielectrophoretic concentration of nanosized viral particles

Yeo, Woon‐Hong; Lee, Hyun Boo; Kim, Jong Hoon; Lee, Kyong Hoon; Chung, Jae Hyun

doi:10.1088/0957-4484/24/18/185502

Cited by 11 publications

(18 citation statements)

References 15 publications

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“…The trajectory of nanoparticles in a medium is studied by considering relevant forces including drag force, Brownian motion force, and DEP. The total force (F N ) is described by [63]:…”

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

“…Multiple particle paths in a medium can be investigated when they are concentrated to a sharp electrode by DEP (Figure 3d) where a nanostructured tip attracts nanoparticles, dispersed in a solution drop. This DEP method using an alternating current (AC) electric field has successfully concentrated low-abundance nanoparticles such as T7 phage [63,66], DNA [67,68], gold [69], and oligonucleotides [70,71]. A schematic illustration in Figure 3e [63] captures the working principle of DEP for concentration of nanoparticles in a medium.…”

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

“…This DEP method using an alternating current (AC) electric field has successfully concentrated low-abundance nanoparticles such as T7 phage [63,66], DNA [67,68], gold [69], and oligonucleotides [70,71]. A schematic illustration in Figure 3e [63] captures the working principle of DEP for concentration of nanoparticles in a medium. A dendritic tip, comprised of silicon carbide nanowires wrapped with single-walled carbon nanotubes, is immersed in a 2 µL sample droplet opposite a metal coil.…”

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

“…where ε * , ε, σ, and ω are the complex permittivity, the DC permittivity, the conductivity, and the applied frequency, respectively. Collectively, the particle travel path and concentration speed can be estimated by substituting Equations (15)- (17) into Equation (14): [73] n/a 1-10 mL 10 2 vp/mL 10 5 vp/mL SV-40, HSV-1, PPV, poliovirus, HAV, HBV, HCV [74] 15 min 1 mL 10 3 vp/mL (2) T7 bacteriophage [63] 5 min. (4) 2 µL 10 4 vp/mL Influenza A [75] 10-20 min n/a 1 vp Influenza virus [76] 5 min (5) n/a 5 µL 10 2 RNA copies/µL (2) 8-12 genomic copies (6) SARS, DENV, JEV, Influenza A, human adenovirus [78] 20 min 1 µL 6 × 10 2 DNA copies/µL (7) [moles] Japanese encephalitis virus (JEV) [79] n/a 2 mL 0.32 nM n/a n/a Influenza A (H7N7) [80] 320 s 1 µL n/a n/a (1) Results based on pure or spiked serum samples; (2) Lowest demonstrated.…”

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

See 3 more Smart Citations

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Dincau

Yong-Kuk

Kim

et al. 2017

Sensors

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Early disease diagnostics require rapid, sensitive, and selective detection methods for target analytes. Specifically, early viral detection in a point-of-care setting is critical in preventing epidemics and the spread of disease. However, conventional methods such as enzyme-linked immunosorbent assays or cell cultures are cumbersome and difficult for field use due to the requirements of extensive lab equipment and highly trained personnel, as well as limited sensitivity. Recent advances in nanoparticle concentration have given rise to many novel detection methodologies, which address the shortcomings in modern clinical assays. Here, we review the primary, well-characterized methods for nanoparticle concentration in the context of viral detection via diffusion, centrifugation and microfiltration, electric and magnetic fields, and nano-microfluidics. Details of the concentration mechanisms and examples of related applications provide valuable information to design portable, integrated sensors. This study reviews a wide range of concentration techniques and compares their advantages and disadvantages with respect to viral particle detection. We conclude by highlighting selected concentration methods and devices for next-generation biosensing systems.

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“…The trajectory of nanoparticles in a medium is studied by considering relevant forces including drag force, Brownian motion force, and DEP. The total force (F N ) is described by [63]:…”

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

Section: Electric and Magnetic Fieldsmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Dincau

Yong-Kuk

Kim

et al. 2017

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…Once pre-concentrated, the detection can be carried out by fluorescence and electrical measurement [147]. Such an amplification-free platform can show a high sensitivity equivalent to polymerase chain reaction but with a short assay time [148]. The nanostructured tip offers a simple configuration for POC diagnosis as well as a convenient home-diagnostic platform, and mechanical analysis plays an important role in rapid development of novel tip designs for specific problems.…”

Section: Point-of-care Diagnostics Using Nanosensorsmentioning

confidence: 99%

USNCTAM perspectives on mechanics in medicine

Bao

Bazilevs

Chung

et al. 2014

J. R. Soc. Interface.

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Over decades, the theoretical and applied mechanics community has developed sophisticated approaches for analysing the behaviour of complex engineering systems. Most of these approaches have targeted systems in the transportation, materials, defence and energy industries. Applying and further developing engineering approaches for understanding, predicting and modulating the response of complicated biomedical processes not only holds great promise in meeting societal needs, but also poses serious challenges. This report, prepared for the US National Committee on Theoretical and Applied Mechanics, aims to identify the most pressing challenges in biological sciences and medicine that can be tackled within the broad field of mechanics. This echoes and complements a number of national and international initiatives aiming at fostering interdisciplinary biomedical research. This report also comments on cultural/educational challenges. Specifically, this report focuses on three major thrusts in which we believe mechanics has and will continue to have a substantial impact. (i) Rationally engineering injectable nano/microdevices for imaging and therapy of disease. Within this context, we discuss nanoparticle carrier design, vascular transport and adhesion, endocytosis and tumour growth in response to therapy, as well as uncertainty quantification techniques to better connect models and experiments. (ii) Design of biomedical devices, including point-of-care diagnostic systems, model organ and multi-organ microdevices, and pulsatile ventricular assistant devices. (iii) Mechanics of cellular processes, including mechanosensing and mechanotransduction, improved characterization of cellular constitutive behaviour, and microfluidic systems for single-cell studies.

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Exterior‐electrode electrically driven microconcentrator

2018

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This study uses negative dielectrophoresis and AC electroosmosis as a driving mechanism and presents an electrically driven microconcentrator that concentrates the sample in the region exterior to the electrodes (termed as exterior-electrode electrically driven microconcentrator in this paper). The proposed microconcentrator uses a 3-D face-to-face electrode pair; the top electrode is a relatively large planar electrode, and the bottom electrode is formed with three to six long and thin electrodes connected into an open ring. The sample is brought to the vicinity of the open electrode at the bottom by electroosmotic flow; then, negative dielectrophoresis is used to push the sample away from the electrode and concentrate it in the region surrounded by the open ring electrode. Concentration using an exterior-electrode electrically driven microconcentrator offers promise for convenient use in conjunction with relevant detection systems. The results indicate that for the proposed exterior-electrode electrically driven microconcentrator, the optimal frequency is 100 kHz and the optimal voltage is 13 V . The corner concentration process at the corners of the bottom open electrodes enables the multi-corner electrodes to exhibit better concentration results than that exhibited by semicircular-shaped electrodes. The concentration performance is most favorable when the shape of the open electrode at the bottom is a five-vertex electrode, enabling a concentration enhancement factor of 55 times for a latex particle solution and 11 times for E. coli. The experimental results also demonstrate that the concentration phenomenon in this study is not induced by non-specific adsorption and can be repeated multiple times.

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Nanotip analysis for dielectrophoretic concentration of nanosized viral particles

Cited by 11 publications

References 15 publications

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

Recent Advances in Nanoparticle Concentration and Their Application in Viral Detection Using Integrated Sensors

USNCTAM perspectives on mechanics in medicine

Exterior‐electrode electrically driven microconcentrator

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