Nanotopographical control for maintaining undifferentiated human embryonic stem cell colonies in feeder free conditions

Bae, Daekyeong; Moon, Sung; Park, Bo Gi; Park, Soon‐Jung; Jung, Taek-Hee; Kim, Jung Suk; Lee, Kyu Back; Chung, Hyung Min

doi:10.1016/j.biomaterials.2013.10.031

Cited by 64 publications

(64 citation statements)

References 64 publications

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“…One would presume that the same fabrication techniques used by other research groups to construct nanoscale topographies for studies with mammalian cells, could just as easily be adopted for the use of bacterial cells. However, many of those nanostructured surfaces were fabricated with the intent of replicating the 3D scaffold-like structure of the extra cellular matrix (ECM) in efforts of promoting mammalian cell differentiation and proliferation [93][94][95][96][97]. This is quite different than the motivation for antifouling work, which focuses on limiting and preventing bacterial attachment to surfaces.…”

Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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Bacterial surface fouling is problematic for a wide range of applications and industries, including, but not limited to medical devices (implants, replacement joints, stents, pacemakers), municipal infrastructure (pipes, wastewater treatment), food production (food processing surfaces, processing equipment), and transportation (ship hulls, aircraft fuel tanks). One method to combat bacterial biofouling is to modify the topographical structure of the surface in question, thereby limiting the ability of individual cells to attach to the surface, colonize, and form biofilms. Multiple research groups have demonstrated that micro and nanoscale topographies significantly reduce bacterial biofouling, for both individual cells and bacterial biofilms. Antifouling strategies that utilize engineered topographical surface features with well-defined dimensions and shapes have demonstrated a greater degree of controllable inhibition over initial cell attachment, in comparison to undefined, texturized, or porous surfaces. This review article will explore the various approaches and techniques used by researches, including work from our own group, and the underlying physical properties of these highly structured, engineered micro/nanoscale topographies that significantly impact bacterial surface attachment.

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Section: Pushing Antifouling Topography To the Nanoscalementioning

confidence: 99%

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

2014

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“…The colony morphology was spherical with fine peripheral outgrowths of filopodia and limited protrusions of lamellopodia, which was contrasted to the flat and broad lamellopodia observed in mid (170-290 nm) and high (290-360 nm) regions ( Fig. 4.5) [144]. The limited cell-substrate interaction on the small pillars hindered cell spreading, but forced the cells to rely more on cell-cell interaction mediated by E-cadherin, a phenomenon closely related to pluripotency of ESCs.…”

Section: Nano-/micro-topographymentioning

confidence: 78%

“…Creating well-defined micro-or nano-patterns provides nano-/micro-topological cues to control the fate of cells. In the previous part of PSC self-renewal, the nano-grooves and nano-pillars tailored at certain sizes have already been demonstrated to be effective in preserving the pluripotency, through the change in PSC integrin interactions with underlying nano-recognition sites, and consequently the altered cell-cell interactions and cellular aggregation [142][143][144]146]. For the PSC differentiation, not only the initial integrin-ligand interactions but the distribution of patterns of the nano-/ micro-topologies that can affect the middle-and long-term behaviors, such as polarization, migration, and cytoskeletal elongation is also important.…”

Section: Nano-/micro-topographymentioning

confidence: 99%

Biomaterials control of pluripotent stem cell fate for regenerative therapy

Pérez

Choi

Han

et al. 2016

Progress in Materials Science

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“…For example in hES cells cultured on nano-roughened and smooth glass substrates (Chen et al, 2012) maintenance of pluripotency was found to depend on an interplay between focal adhesion formation, cell–cell contacts and cytoskeletal rearrangements mediated by non-muscle myosin IIa (NMMIIa) on flat surfaces. On the other hand, nanotopographic pillars of hexagonal versus honeycomb arrangements (Kong et al, 2013) and nanopillar gradients of varied spacing (Bae et al, 2014) supported Oct4 + cells and maintained E-cadherin expression. Moreover, focal adhesion kinase (FAK) inactivation led to a more dynamic reorganization of the cytoskeleton on the topographies of the lowest diameters.…”

Section: The Role Of the Microenvironment And Synthetic Substratesmentioning

confidence: 99%

Human pluripotent stem cells on artificial microenvironments: a high content perspective

et al. 2014

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Self-renewing stem cell populations are increasingly considered as resources for cell therapy and tools for drug discovery. Human pluripotent stem (hPS) cells in particular offer a virtually unlimited reservoir of homogeneous cells and can be differentiated toward diverse lineages. Many diseases show impairment in self-renewal or differentiation, abnormal lineage choice or other aberrant cell behavior in response to chemical or physical cues. To investigate these responses, there is a growing interest in the development of specific assays using hPS cells, artificial microenvironments and high content analysis. Several hurdles need to be overcome that can be grouped into three areas: (i) availability of robust, homogeneous, and consistent cell populations as a starting point; (ii) appropriate understanding and use of chemical and physical microenvironments; (iii) development of assays that dissect the complexity of cell populations in tissues while mirroring specific aspects of their behavior. Here we review recent progress in the culture of hPS cells and we detail the importance of the environment surrounding the cells with a focus on synthetic material and suitable high content analysis approaches. The technologies described, if properly combined, have the potential to create a paradigm shift in the way diseases are modeled and drug discovery is performed.

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Nanotopographical control for maintaining undifferentiated human embryonic stem cell colonies in feeder free conditions

Cited by 64 publications

References 64 publications

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Nano and Microscale Topographies for the Prevention of Bacterial Surface Fouling

Biomaterials control of pluripotent stem cell fate for regenerative therapy

Human pluripotent stem cells on artificial microenvironments: a high content perspective

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