Nanotraps for the containment and clearance of SARS-CoV-2

Chen, Min; Rosenberg, Jillian; Cai, Xiaolei; Lee, Andy Chao Hsuan; Shi, Jiuyun; Nguyen, Mindy; Wignakumar, Thirushan; Mirle, Vikranth; Edobor, Arianna Joy; Fung, John J.; Donington, Jessica S.; Shanmugarajah, Kumaran; Lin, Yiliang; Chang, Eugene B.; Randall, Glenn; Penaloza-MacMaster, Pablo; Tian, Bozhi; Madariaga, Maria Lucia; Huang, Jun

doi:10.1016/j.matt.2021.04.005

Cited by 45 publications

(32 citation statements)

References 78 publications

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“…Most notably, they encapsulate messenger ribonucleic acid (mRNA) in the Moderna and Pfizer‐BioNTech [ 6 ] COVID‐19 vaccines. As well, they have also been functionalized with angiotensin‐converting enzyme 2 (ACE2) or neutralizing antibodies as a strategy to clear severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), [ 7 ] the enveloped coronavirus responsible for COVID‐19. Because enveloped viruses have been shown to fuse with liposomes, [ 8 ] lipid‐based nanoparticles have been suggested as models of enveloped viruses, themselves.…”

Section: Introductionmentioning

confidence: 99%

DNA‐Directed Patterning for Versatile Validation and Characterization of a Lipid‐Based Nanoparticle Model of SARS‐CoV‐2

2021

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Lipid‐based nanoparticles have been applied extensively in drug delivery and vaccine strategies and are finding diverse applications in the coronavirus disease 2019 (COVID‐19) pandemic—from vaccine‐component encapsulation to modeling the virus, itself. High‐throughput, highly flexible methods for characterization are of great benefit to the development of liposomes featuring surface proteins. DNA‐directed patterning is one such method that offers versatility in immobilizing and segregating lipid‐based nanoparticles for subsequent analysis. Here, oligonucleotides are selectively conjugated onto a glass substrate and then hybridized to complementary oligonucleotides tagged to liposomes, patterning them with great control and precision. The power of DNA‐directed patterning is demonstrated by characterizing a novel recapitulative lipid‐based nanoparticle model of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2)—S–liposomes—that presents the SARS‐CoV‐2 spike (S) protein on its surface. Patterning a mixture of S–liposomes and liposomes that display the tetraspanin CD63 to discrete regions of a substrate shows that angiotensin‐converting enzyme 2 (ACE2) specifically binds to S–liposomes. Subsequent introduction of S–liposomes to ACE2‐expressing cells tests the biological function of S–liposomes and shows agreement with DNA‐directed patterning‐based assays. Finally, multiplexed patterning of S–liposomes verifies the performance of commercially available neutralizing antibodies against the two S variants. Overall, DNA‐directed patterning enables a wide variety of custom assays for the characterization of any lipid‐based nanoparticle.

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Section: Introductionmentioning

confidence: 99%

DNA‐Directed Patterning for Versatile Validation and Characterization of a Lipid‐Based Nanoparticle Model of SARS‐CoV‐2

2021

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“…In a recent study, liposomal-based Nanotrap surfaces were functionalized with SARS-CoV-2 neutralizing antibody and phagocytosis-specific phosphatidylserines ( Chen et al, 2021 ). This Nanotrap has been found to completely inhibit SARS-CoV-2 infection by blocking the binding of S protein to ACE2 of host cells.…”

Section: Prevention and Antiviral Treatment By Functionalized Npsmentioning

confidence: 99%

“…This Nanotrap has been found to completely inhibit SARS-CoV-2 infection by blocking the binding of S protein to ACE2 of host cells. It significantly inhibited the infection by a pseudotyped and authentic SARS-CoV-2 in an ex vivo lung perfusion system ( Chen et al, 2021 ). Moreover, this Nanotrap promotes the phagocytosis of macrophages without being infected themselves after surface modification of the phagocyte-specific phosphatidylserine ligands.…”

Section: Prevention and Antiviral Treatment By Functionalized Npsmentioning

confidence: 99%

“…For a desirable targeted therapy, the antiviral compound should not only be delivered to a specific tissue but should be accurately delivered to the virus-infected cells. In this case, antiviral agents can inhibit the virus in different ways, such as blocking the binding of the virus to the receptor on the cell membrane, inhibiting the replication of the virus in the cytoplasm, and inhibiting viral budding ( Li et al, 2016 ; Park et al, 2016 ; Chen et al, 2021 ). Thus, targeted delivery of infected cells plays an essential role in promoting the therapeutic effect of antiviral drugs.…”

Section: Introductionmentioning

confidence: 99%

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Functionalized Nanoparticles in Prevention and Targeted Therapy of Viral Diseases With Neurotropism Properties, Special Insight on COVID-19

et al. 2021

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Neurotropic viruses have neural-invasive and neurovirulent properties to damage the central nervous system (CNS), leading to humans’ fatal symptoms. Neurotropic viruses comprise a lot of viruses, such as Zika virus (ZIKV), herpes simplex virus (HSV), rabies virus (RABV), and severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). Effective therapy is needed to prevent infection by these viruses in vivo and in vitro. However, the blood-brain barrier (BBB) usually prevents macromolecules from entering the CNS, which challenges the usage of the traditional probes, antiviral drugs, or neutralizing antibodies in the CNS. Functionalized nanoparticles (NPs) have been increasingly reported in the targeted therapy of neurotropic viruses due to their sensitivity and targeting characteristics. Therefore, the present review outlines efficient functionalized NPs to further understand the recent trends, challenges, and prospects of these materials.

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“…The nanotraps blocked SARS-CoV-2 infection in host cells by preventing the binding of the SARS-spike CoV-2 protein with the ACE2 protein. At least two anti-SARS-CoV-2 neutralizing antibodies and phagocytosis-specific phosphatidylserines were added to the liposomal-based nanotrap surfaces [29]. A/H1N1, SARS-CoV-2, and MERS-CoV are some of the new emergent infectious illnesses for which nanomedicine is being employed [30].…”

Section: Regeneration Of Lung Tissues Using Biopolymeric Nanoparticles For Covid-19 Patientsmentioning

confidence: 99%

The Potential Contribution of Biopolymeric Particles in Lung Tissue Regeneration of COVID-19 Patients

et al. 2021

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The lung is a vital organ that houses the alveoli, which is where gas exchange takes place. The COVID-19 illness attacks lung cells directly, creating significant inflammation and resulting in their inability to function. To return to the nature of their job, it may be essential to rejuvenate the afflicted lung cells. This is difficult because lung cells need a long time to rebuild and resume their function. Biopolymeric particles are the most effective means to transfer developing treatments to airway epithelial cells and then regenerate infected lung cells, which is one of the most significant symptoms connected with COVID-19. Delivering biocompatible and degradable natural biological materials, chemotherapeutic drugs, vaccines, proteins, antibodies, nucleic acids, and diagnostic agents are all examples of these molecules‘ usage. Furthermore, they are created by using several structural components, which allows them to effectively connect with these cells. We highlight their most recent uses in lung tissue regeneration in this review. These particles are classified into three groups: biopolymeric nanoparticles, biopolymeric stem cell materials, and biopolymeric scaffolds. The techniques and processes for regenerating lung tissue will be thoroughly explored.

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Nanotraps for the containment and clearance of SARS-CoV-2

Cited by 45 publications

References 78 publications

DNA‐Directed Patterning for Versatile Validation and Characterization of a Lipid‐Based Nanoparticle Model of SARS‐CoV‐2

DNA‐Directed Patterning for Versatile Validation and Characterization of a Lipid‐Based Nanoparticle Model of SARS‐CoV‐2

Functionalized Nanoparticles in Prevention and Targeted Therapy of Viral Diseases With Neurotropism Properties, Special Insight on COVID-19

The Potential Contribution of Biopolymeric Particles in Lung Tissue Regeneration of COVID-19 Patients

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