Nanozyme‐based luminescence detection

Zhang, Jinyi; Liu, Juewen

doi:10.1002/bio.3893

Cited by 33 publications

(23 citation statements)

References 123 publications

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“…Nanozymes, nanomaterials with inherent enzyme-mimetic properties, , exhibit many unique merits over biological enzymes, including ease of large-scale preparation, low cost, and high stability under severe environments. − So far, with the discovery of new features, various nanozymes have been applied successfully in different fields, such as biosensing, , cancer therapy, , food safety, sterilization, and environmental treatment . Given the importance of organophosphorus pesticide detection, nanozymes have been widely used in various pesticide assay methods based on the enzyme-inhibition strategy, where cholinesterase , or cholinesterase-choline oxidase (ChOx) , catalysis systems were generally used.…”

Section: Introductionmentioning

confidence: 99%

Selective Inhibition toward Dual Enzyme-like Activities of Iridium Nanozymes for a Specific Colorimetric Assay of Malathion without Enzymes

Liang

Wang

Zhang

et al. 2022

J. Agric. Food Chem.

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A colorimetric assay based on an enzyme-inhibition strategy is promising for the on-site detection of pesticide residues. Due to the high cost and low stability of enzymes, nanozymes (nanomaterials with enzyme-like activities) are widely developed as substitutes of enzymes. However, the inhibition of pesticides toward enzymes and nanozymes generally lacks selectivity. It is of great significance and challenge to design a specific pesticide assay based on an activity-inhibition strategy. Here, we discovered that iridium nanoparticles possess both peroxidase-like and oxidase-like activities under the same conditions, and their catalytic mechanisms are different. The synergistic effect of dual enzyme-like activities enhanced the colorimetric signal. Interestingly, the dual enzyme-mimicking activities could be simultaneously inhibited, and the inhibition effect exhibited high selectivity toward malathion. Considering the popularity and the hazards of malathion, a malathion assay method based on activity inhibition was established without enzymes and a redundant process. The synergistic effect of the selective inhibition of dual enzyme-like activities enhanced the selectivity and sensitivity. The proposed assay strategy opens up an avenue for specific assay of various pesticides.

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Section: Introductionmentioning

confidence: 99%

Selective Inhibition toward Dual Enzyme-like Activities of Iridium Nanozymes for a Specific Colorimetric Assay of Malathion without Enzymes

Liang

Wang

Zhang

et al. 2022

J. Agric. Food Chem.

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“…It is well-known that AuNPs act as a catalyst to decompose hydrogen peroxide to form • OH, which can react with luminol to enhance CL. 29,30 Based on this catalytic reaction, Cui and co-workers proposed a series of luminol-type CL reagent-functionalized AuNPs. These functionalized AuNPs with excellent CL performance had been successfully applied in immunoassays and DNA assays.…”

Section: ■ Introductionmentioning

confidence: 99%

Flower-like Gold Nanoparticles for In Situ Tailoring Luminescent Molecules for Synergistic Enhanced Chemiluminescence

Zhang

Song

et al. 2022

Anal. Chem.

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In recent years, gold nanoparticles (AuNPs) have attracted much attention due to their ease of surface modification, excellent biocompatibility, and extraordinary optoelectronic and catalytic activities. Herein, based on a AuNP-catalyzed reaction, a strategy for tailoring luminescent molecules in situ is proposed to trigger an ultrastrong chemiluminescence (CL). In the strategy, flower-like AuNPs are prepared using CL molecular probes (Probe-OH for NaClO/ONOO–) via one-pot synthesis and subsequently act as a tailor for Probe-OH to generate novel CL molecules, allowing a synergistic CL enhancement about 4 times that of initial Probe-OH. Furthermore, by modification with poly(vinylpyrrolidone) (PVP) on the surface, the CL signals (only for NaClO) are amplified by 100 times based on an intermolecular chemically initiated electron exchange luminescence (CIEEL) mechanism. Given the improved sensitivity and selectivity over Probe-OH, the thus-formed CIEEL nanoplatform (PVP-Au) is successfully developed for detecting NaClO in a wide range of 2.5–100 μM, and the detection limit is 10.68 nM. This work provides unprecedented perspectives for expanding this facile and effective strategy for CL amplification based on AuNP catalysis.

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“…The direct chemiluminescence production, obtained after the cleavage of the trigger, enables the set-up of simple methods for enzymatic activity evaluation. This issue allows us, indeed, to overcome the use of multi enzymatic step reactions, as in the case of luciferin derivative probes [ 7 , 29 ], or secondary chemical reactions, such as oxidation in the case of luminol derivatives, as the base of numerous currently used methods [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity

Rocca

Mingione

Casati

et al. 2022

IJMS

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The set-up of highly sensitive detection tools to evaluate lipase activity remains a central goal in different fields. In this context, we proposed new chemiluminescent 1,2-dioxetane luminophores, sharing an octanoyl triggerable group, to monitor lipase activity. We herein report the synthesis and both the evaluation of their luminescence emission profile and their enzyme–substrate specificity, generated by three different commercial lipases (Candida cylindracea, Pseudomonas fluorescens, and Mucor miehei) and one esterase (porcine liver esterase, PLE, as a literature control). Remarkably, the present study confirmed the applicability of these 1,2-dioxetane luminophores as (i) highly efficient, broad-range, chemiluminescent probes for the detection and the enzymatic activity evaluation of lipases and as (ii) promising candidates for the future development of both flash- and glow-type luminescence assays.

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Nanozyme‐based luminescence detection

Cited by 33 publications

References 123 publications

Selective Inhibition toward Dual Enzyme-like Activities of Iridium Nanozymes for a Specific Colorimetric Assay of Malathion without Enzymes

Selective Inhibition toward Dual Enzyme-like Activities of Iridium Nanozymes for a Specific Colorimetric Assay of Malathion without Enzymes

Flower-like Gold Nanoparticles for In Situ Tailoring Luminescent Molecules for Synergistic Enhanced Chemiluminescence

Small-Molecules as Chemiluminescent Probes to Detect Lipase Activity

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