Naphthalene-2-sulfonate induced toxicity in blood cells of freshwater fish Channa punctatus using comet assay, micronucleus assay and ATIR-FTIR approach

Mehra, Sukanya; Chadha, Pooja

doi:10.1016/j.chemosphere.2020.129147

Cited by 29 publications

(8 citation statements)

References 44 publications

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“…SEM studies of erythrocytes revealed various abnormalities such as irregular shape, notched, elongated cells, cytoplasmic bleb in C. punctatus . Various researchers observed different abnormalities in erythrocytes of fish exposed to different xenobiotics ( Dey et al, 2016 , Kaur and Kaur, 2015 , Mehra and Chadha, 2021 ). Changes in the morphology of erythrocytes of fish exposed to xenobiotics indicate the poor health status of the fish ( Sawhney and Johal, 2000 ).…”

Section: Discussionmentioning

confidence: 99%

Bisphenol A induced toxicity in blood cells of freshwater fish Channa punctatus after acute exposure

Sharma

Chadha

2021

Saudi Journal of Biological Sciences

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Section: Discussionmentioning

confidence: 99%

Bisphenol A induced toxicity in blood cells of freshwater fish Channa punctatus after acute exposure

Sharma

Chadha

2021

Saudi Journal of Biological Sciences

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“…Both genomic damage (assessed by the comet assay) and chromosomal damage (assessed by the micronucleus assay, for example) can be classified as genotoxic damage [ 8 , 9 ]. Genomic damage can be repaired, and chromosomal is already fixed in the cellular genome [ 10 ]. Notably, genomic damage can evolve into chromosomal damage [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

3-Heptylidene-4,6-Dimethoxy-3H-Isobenzofuran-1-One Is Genotoxic, Increases the Frequency of Cell Death, and Potentiates the Effects of Cyclophosphamide and Cisplatin

et al. 2023

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3-heptylidene-4,6-dimethoxy-3H-isobenzofuran-1-one (Phthalide 1) is the precursor of three resorcinol lipids that have been described as potential chemotherapeutic agents and capable of potentiating the effects of cyclophosphamide. In this study, we evaluated the genotoxic potential, cell-killing potential, and interactions with cyclophosphamide and cisplatin of phthalide 1. Twelve groups were created from 120 mice: Negative Control, cyclophosphamide (100 mg/kg), cisplatin (6 mg/kg), Phthalide 1 (5, 10 and 20 mg/kg), and associations of 1 with cyclophosphamide and 1 with cisplatin. The results demonstrate that 1 increases (p < 0.05) the frequency of chromosomal damage, liver and kidney cell death, and splenic phagocytosis. The association of 1 with cyclophosphamide and cisplatin demonstrated a chemopreventive effect and, therefore, a reduction (p < 0.05) in the frequency of chromosomal damage. However, cell death and splenic phagocytosis did not suffer significant variations. As a result of the above, 1 has potential chemotherapeutic application and may be a candidate for developing a new generation of chemotherapeutics. In addition, it has characteristics to be used as a chemotherapy adjuvant in association with cyclophosphamide and cisplatin since it increases the frequency of cell death induced by chemotherapy. We also reported that the chemopreventive effect of 1, in association with cyclophosphamide and cisplatin, can prevent adverse effects (induction of DNA damage in non-tumor cells) without interfering with the mode of action of chemotherapy drugs and, therefore, without reducing the induction of cell death.

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“…Damage dropped to control value suggested the possibility of complete turnover of fish erythrocytes and other cells, as the life span of erythrocytes in fish is from 1-3 months [53] . Various authors have supported the use of percent tail DNA as most appropriate parameters [37,[54][55][56][57][58] . Tail length is used to test genotoxicity in a number of studies [57][58][59] .…”

Section: Discussionmentioning

confidence: 99%

“…Various authors have supported the use of percent tail DNA as most appropriate parameters [37,[54][55][56][57][58] . Tail length is used to test genotoxicity in a number of studies [57][58][59] . Several workers scored nuclear abnormalities along with micronuclei after exposure to nanoparticles [25,48,50,60,61,62] .…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress and genotoxicity assessment of silicon di oxide nanoparticle in snake headed murrel, Channa punctautus

Verma¹,

Verma²,

Park³

2021

Int. J. Appl. Res.

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Consequences of waterborne silicon di oxide nanoparticles (SiO2 NPs) on snake headed fish, Channa punctatus were investigated and analyzed for oxidative stress and genotoxicity in a long-term assay. Fish were divided into three groups: Group I (control), Group II and III considered as treated groups. Fish were exposed to 25% and 50% of LC50 value (60 and 120 mg/l of SiO2 NPs) at day 7,15,21,28 and 35. Fish exposed to higher concentrations of SiO2 NPs showed major alterations in oxidative stress markers. Significant alterations were noted in SOD, CAT, GST, GSH, GPx and GR in the gills, liver and kidney of fish of both treated groups. A significant increase of SOD, CAT, GST, GPx and GR in the gill, liver and kidney of fish was observed, whereas GSH showed significant decrease in all treated groups in all tissues. LPO was also estimated in gills, liver and kidney and elevation was noted. By standard genotoxicity test such as micronucleus test and comet assay, genetic damage (cytoplasmic, nuclear and DNA damage) was recorded which indicates significant toxicity of SiO2 NPs at higher concentrations. Significant changes in all parameters studied thus reflected an adverse influence of SiO2 Nps exposure on oxidative stress as well as genotoxicity of the fish. SiO2 Nps toxicity appeared to be dose and duration dependent. The presence of SiO2 Nps leads to an activation of the antioxidant defense system and the occurrence of lipid and genetic damage if antioxidant enzymes were unable to overcome oxidative stress.

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Naphthalene-2-sulfonate induced toxicity in blood cells of freshwater fish Channa punctatus using comet assay, micronucleus assay and ATIR-FTIR approach

Cited by 29 publications

References 44 publications

Bisphenol A induced toxicity in blood cells of freshwater fish Channa punctatus after acute exposure

Bisphenol A induced toxicity in blood cells of freshwater fish Channa punctatus after acute exposure

3-Heptylidene-4,6-Dimethoxy-3H-Isobenzofuran-1-One Is Genotoxic, Increases the Frequency of Cell Death, and Potentiates the Effects of Cyclophosphamide and Cisplatin

Oxidative stress and genotoxicity assessment of silicon di oxide nanoparticle in snake headed murrel, Channa punctautus

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