Naringin ameliorates gentamicin-induced nephrotoxicity and associated mitochondrial dysfunction, apoptosis and inflammation in rats: Possible mechanism of nephroprotection

Sahu, Bidya Dhar; Tatireddy, Srujana; Koneru, Meghana; Borkar, Roshan M.; Kumar, Jerald Mahesh; Kuncha, Madhusudana; Srinivas, R.; Sunder, R. Shyam; Sistla, Ramakrishna

doi:10.1016/j.taap.2014.02.022

Cited by 131 publications

(68 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[41][42][43][44] Several lines of studies reported that GM-induced GSH level depletion might be due to excess generation of free radicals or increased consumption in the protection of -SH group-containing proteins and decreased SOD antioxidant enzymes associated with overproduction of superoxide anions and hydrogen peroxide. 18,[45][46][47] In consistent with the previous finding, our results showed that GM administration increased MDA level as an end product of lipid peroxidation while decreased GSH and SOD levels in renal tissue. 48 The present finding indicated that berberine treatment restore the MDA, GSH and SOD levels in renal tissue of GM-induced toxicity groups via its free radical scavenging and/or increasing antioxidant property.…”

Section: Discussionsupporting

confidence: 93%

“…35 Various studies revealed that oxidative stress induced by GM play a major role in the renal structural and functional deterioration by altering biochemical indicators. 18 In this study, GM-induced nephrotoxicity was manifested by elevated serum creatinine and BUN levels. Although berberine an antioxidant attenuates the elevated serum creatinine and BUN levels in GM-induced renal toxicity.…”

Section: Discussionmentioning

confidence: 62%

“…Indeed, it has been reported that GM administration hampers the mitochondrial function in the kidney through reduction of complex-I and complex-IV enzyme activities. 18 Treatment with berberine favorably …”

Section: Discussionmentioning

confidence: 99%

“…Nephrotoxicity was induced in rats (except normal) by GM at a dose of 120 mg/kg, intraperitoneally for 7 days. 18 GM was dissolved in normal saline. Doses of berberine (20 mg/kg and 40 mg/kg) were selected based on the previous study carried out in our laboratory.…”

Section: Experimental Designmentioning

confidence: 99%

See 3 more Smart Citations

Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction

et al. 2016

View full text Add to dashboard Cite

Background: Gentamicin (GM) is the commonly used antibiotics against Gram-negative infection, but the nephrotoxic potential of drug limit its clinical interest. The aim of this study was to investigate the protective effect of berberine (BER) against GM-induced nephrotoxicity and possible underlying mechanisms. Material and methods: The rats were divided into various group, namely normal, GM-control, GM þ BER (10, 20, and 40 mg/kg). Nephrotoxicity was induced by intraperitoneal administration of GM (120 mg/kg) for 7 consecutive days. BER (10, 20, and 40 mg/kg; p.o.) was also administered for the 7 days. Various biochemical, molecular, and histological parameters were assessed in serum and kidney. Results: GM-administration significantly increased (p < 0.001) the serum creatinine and blood urea nitrogen (BUN) as well as renal malonaldehyde (MDA), nitric oxide (NO) along with Kidney Injury Molecule-1 (KIM-1), Neutrophil gelatinase-associated lipocalin (NGAL), and nuclear factor-kappa B (NF-KB) renal mRNA expressions. In addition, GM also significantly decreased (p < 0.001) the renal superoxide dismutase (SOD), reduced glutathione (GSH), B-cell lymphoma 2 (Bcl-2) mRNA expression, and mitochondrial enzymes (NADH dehydrogenase and cytochrome c oxidase) activities. Rats treated with BER (20 and 40 mg/kg; p.o.) significantly and dose-dependently (p < 0.05 and p < 0.01) restore the altered levels of antioxidant, inflammatory, apoptosis, AKI markers as well as depleted mitochondrial enzymes. Histopathological abbreviations were also ameliorated by BER administration. Conclusion: Berberine exerts renoprotective effects through its anti-oxidant, anti-inflammatory, and anti-apoptotic properties.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

See 2 more Smart Citations

Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In this study, the level of eosinophilic casts, leukocyte infiltration and tubular necrosis significantly increased in nephrotoxic group, while carvacrol therapy only significantly decreased the level of tubular necrosis. In similar studies that considered the effects of different antioxidants, the decrease in the rate of tissue injuries caused by gentamicin-induced-nephrotoxicity by using antioxidants have also been reported [6,16,17,28,36].…”

Section: Discussionmentioning

confidence: 82%

Protective Effect of Carvacrol on Renal Functional and Histopathological Changes in Gentamicin-Induced-Nephrotoxicity in Rats

Ahmadvand

Tavafi

Asadollahi

et al. 2016

Zahedan J Res Med Sci

View full text Add to dashboard Cite

Background: Nephrotoxicity is one of the most important side effects of the use of gentamicin sulphate (GS) resulted in reactive oxygen species generation. Antioxidant compounds played effective roles in reduction of renal injuries caused by using of gentamicin. Carvacrol is a strong antioxidant compound. Objectives: The aim of this study is to explore the effect of carvacrol inhibition in lesions of gentamicin-induced nephrotoxicity. Materials and Methods:In this experimental study, 32 male mature Sprague-Dawley rats were divided into 4 groups of 8; group1: control, group 2 sham received daily carvacrol injection (74 mg/kg) for 12 days, group 3 received daily GS injection (100 mg/kg) for 12 days, group 4 received daily GS (100 mg/kg) and carvacrol (74 mg/kg) for 12 days. After 12 days, rats were anaesthetized, blood sample were obtained and kidneys were removed then stained with hematoxylin and eosin method and then were studied histophatologically. Serum creatinine and urea were measured. Results: Flow treatment of nephrotoxic animals with carvacrol could significantly inhibit leukocyte infiltration (9.42%) and tubular necrosis (38.18%) in comparison with the nephrotoxic untreated group. Carvacrol significantly decreased the levels of urea and creatinine in treated group compared with the nephrotoxic untreated group. Conclusions: The findings showed that carvacrol alleviates loss of leukocyte infiltration (9.42%) and tubular necrosis and exerts beneficial effects on kidney function test in nephrotoxic group.

show abstract

The protective effects of rutin on the liver, kidneys, and heart by counteracting organ toxicity caused by synthetic and natural compounds

Rahmani

Naraki

Roohbakhsh

et al. 2022

Food Science & Nutrition

View full text Add to dashboard Cite

Rutin is a flavonoid present in many plant species. Because of its antioxidant, anti‐inflammatory, and antiapoptotic properties, rutin is of interest for its potential protective effects against toxic agents. The hepatoprotective, renoprotective, and cardioprotective effects of rutin are reviewed. The antioxidant effects of rutin are elicited by enhancing antioxidant enzymes such as GST, GGT, CAT, GPx, SOD, and GR, activating the Nrf2/HO‐1 pathway, elevating GSH content, and the reduction in MDA. The anti‐inflammatory effects of rutin are mediated by the inhibition of IL‐1β, IL‐6, TGF‐β1, COX‐2, iNOS, TLR4, and XO. Rutin exerted its antiapoptotic effects by inhibition of free radicals, caspase‐3/‐7/‐9, hsp70, HMGB1, and p53, and the elevation of the antiapoptotic protein Bcl‐2. Rutin has potential therapeutic effectiveness against several toxicants, and its beneficial effects are more than likely mediated by its antioxidant, anti‐inflammatory, and/or antiapoptotic property.

show abstract

Naringin ameliorates gentamicin-induced nephrotoxicity and associated mitochondrial dysfunction, apoptosis and inflammation in rats: Possible mechanism of nephroprotection

Cited by 131 publications

References 50 publications

Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction

Ameliorative effect of berberine against gentamicin-induced nephrotoxicity in rats via attenuation of oxidative stress, inflammation, apoptosis and mitochondrial dysfunction

Protective Effect of Carvacrol on Renal Functional and Histopathological Changes in Gentamicin-Induced-Nephrotoxicity in Rats

The protective effects of rutin on the liver, kidneys, and heart by counteracting organ toxicity caused by synthetic and natural compounds

Contact Info

Product

Resources

About