Narrow Genetic Variability in Cicer arietinum L. as Revealed by RFLP Analysis

Udupa, Sripada M.; Sharma, Anuradha; Sharma, R. P.; Pai, R. A.

doi:10.1007/bf03262930

Cited by 74 publications

(51 citation statements)

References 20 publications

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“…Cultivated Cicer arietinum L. is a self-pollinated plant species with low intra-and inter-population variation (Crawford 1990). This was also demonstrated in several studies performed with isoenzymes (Labdi et al 1996;Ahmad et al 2004), seed storage proteins (Ahmad & Slinkard 1992), and RFLPs (Udupa et al 1993. PCR-based techniques and revealing of microsatellite sequences in plant genomes helped to overcome problems with low polymorphism detected also in cultivated chickpea.…”

mentioning

confidence: 76%

Enrichment of chickpea genetic resources collection monitored by microsatellites

Jomová¹,

Benková²,

Kraic³

2009

CZECH J GENET PLANT

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A set of 49 chickpea accessions maintained in the collection of the Genebank of the Slovak Republic was analyzed using microsatellite markers. The level of genetic diversity and the effectivity of new germplasm acquisition for the collection enrichment were evaluated. Five primer pairs used generated 50 different amplified alleles. Four of them, TA2, TA5, TR1, and TR7, containing a long TAA-tandem repeat, were polymorphic with 11-13 alleles per locus. A single fragment was obtained from all the accessions with the primer pair CATPER flanking a small microsatellite repetition in the mRNA sequence of chickpea cationic peroxidase. The genetic diversity was expressed as a diversity index (DI) and polymorphic information content (PIC) with values in the range from 0.885 to 0.904 and from 0.972 to 0.991, respectively. Recently acquired new germplasm contributed 13 new alleles to the entire collection, i.e. almost 70% of microsatellite diversity originated from the recently acquired accessions. Remarkable is also, that one accession carries a unique allel, not present in the remaining collection. Microsatellite analysis revealed the importance of collecting missions for the enrichment of collections of genetic resources.

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mentioning

confidence: 76%

Enrichment of chickpea genetic resources collection monitored by microsatellites

Jomová¹,

Benková²,

Kraic³

2009

CZECH J GENET PLANT

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“…The RFLP and RAPD markers also show very low level of genetic variation between chickpea cultivars (Udupa et al, 1993;Van Rheenen, 1992). The low polymorphism may have been caused by narrow ancestry and self-pollinated nature of the crop (Van Rheenen, 1992).…”

Section: Rflp and Rapd Markersmentioning

confidence: 99%

“…Simon and Muehlbauer (1997) developed an integrated genetic linkage map of chickpea with morphological, isozyme and DNA markers covering the length of 550 cM. Since RFLPs and RAPDs detected low genetic polymorphism in cultivated species (Sant et al, 1999;Udupa et al, 1993), genetic maps based on highly polymorphic marker systems like SSR and AFLP would be of great value in marker assisted selection for trait(s) of interest. A total of 174 microsatellite loci have been reported by Winter et al (1999) and 112 STMS loci distributed over 11 linkage groups covering a length of 613 cM were mapped.…”

Section: Genetic Linkage Mapsmentioning

confidence: 99%

Chickpea Improvement: Role of Wild Species and Genetic Markers

Singh

Sharma

Varshney

et al. 2008

Biotechnology and Genetic Engineering Reviews

111

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Chickpea is an important grain legume of the semi arid tropics and warm temperate zones, and forms one of the major components of human diet. However, a narrow genetic base of cultivated chickpea (Cicer arietinum L.) has hindered the progress in realizing high yield gains in breeding programs. Furthermore, various abiotic and biotic stresses are the major bottlenecks for increasing chickpea productivity. Systematic collection and evaluation of wild species for useful traits has revealed presence of a diverse gene pool for tolerance to the biotic and abiotic stresses. Relationships among the species of genus Cicer are presented based on crossability, karyotype and molecular markers. The reproductive barriers encountered during interspecific hybridization are also examined. Recent information on genetic linkage maps, comparison of isozymes

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“…Unfortunately, the chickpea crop is susceptible to a range of biotic and abiotic stresses, which can be devastating to crop yield. A low level of genetic variability within C. arietinum has hampered chickpea breeders in their efforts to develop widely adapted cultivars with resistance to biotic and abiotic stresses (Ladizinsky and Adler, 1975;Tuwafe et al, 1988;Kazan and Muehlbauer, 1991;Udupa et al, 1993;van Rheenen et al, 1993;Labdi et al,1996;Tayyar and Waines, 1996;Simon and Muehlbauer, 1997;Siddique et al, 2000). Recently, this low level of genetic variability has been attributed to a series of genetic bottlenecks in the domestication of chickpea, including the restricted distribution of the wild progenitor, the founder effect associated with domestication, and the shift from winter to summer cropping .…”

Section: Germplasm Collection Maintenance and Evaluationmentioning

confidence: 99%

“…Later developments on genetic linkage maps in chickpea that started with the work of Simon and Muehlbauer (1997) relied heavily on DNA-based molecular markers. In the lack of more recently available molecular markers, Simon and Muehlbauer (1997) employed RFLP and RAPD markers that show limited polymorphism in the cultivated species (Udupa et al, 1993;Banerjee et al, 1999).…”

Section: Genetic Linkage Mappingmentioning

confidence: 99%