Nasal immunization using a mimovirus vaccine based on the Eppin B-cell epitope induced suppressed fertility in mice

Chen, Zhengqiong; Shen, Zhaohua; Li, Jintao; Wang, He; Yang, Ying; Liang, Zhiqing

doi:10.4161/hv.29200

Cited by 4 publications

(5 citation statements)

References 28 publications

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“…We speculate this may be due to an insufficient immunoreactive intensity. Interestingly, in contrast to results from Chen et al, no obvious differences were observed in the number of the pups born between the peptide group and PBS group . This indicates that peptide immunization only inhibited the mating fertility rates but did not affect the number of pups.…”

Section: Discussioncontrasting

confidence: 85%

“…Interestingly, in contrast to results from Chen et al, no obvious differences were observed in the number of the pups born between the peptide group and PBS group. 26 This indicates that peptide immunization only inhibited the mating fertility rates but did not affect the number of pups. Our study indicates that ZNF185 may be a target candidate for contraceptive vaccines, and this work contributes to the preparation of male contraceptives.…”

Section: Analysis Of Male Reproductive Organsmentioning

confidence: 95%

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ZNF185‐derived peptide induces fertility suppression in mice

Fan

Zhao

Pan

et al. 2018

Journal of Peptide Science

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Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in male reproduction. However, it is unclear whether ZNF185 may be a target candidate for contraceptive vaccines. In this study, antigenic peptides derived from ZNF185 were prepared, and their immune contraceptive effects were investigated using mice. Results from enzyme‐linked immunosorbent assay (ELISAs) showed that peptide immunization induced an antibody titre increase that reached a peak in week 12. Peptide‐3 and peptide‐4 were then chosen for subsequent experiments. The results of the fertility assays showed that peptide immunization inhibited the mating and fertility rates of the mice, whereas there were no obvious changes in the number of pups per litter. Subsequently, epididymal sperm was analysed. The results demonstrated that the sperm count and sperm motility were significantly decreased in the peptide group, while the amount of abnormal sperm was significantly increased in the peptide‐3 group. The male reproductive organs were also evaluated. There were no obvious differences in testis or epididymal weights, in the diameters of the seminiferous tubules, or in the thicknesses of the seminiferous epithelium between the peptide group and the phosphate buffer saline (PBS) group. In addition, histological analysis indicated that there were no obvious pathologic changes in testis and epididymal histology in the peptide group; however, the number of spermatozoa present in the epididymal lumen of the peptide group was significantly decreased when compared with the PBS group. Our study demonstrates for the first time that peptides derived from ZNF185 may induce fertility suppression in mice without damaging reproductive organs. These peptides have the potential to be used as a male contraceptive vaccine.

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Section: Discussioncontrasting

confidence: 85%

Section: Analysis Of Male Reproductive Organsmentioning

confidence: 95%

ZNF185‐derived peptide induces fertility suppression in mice

Fan

Zhao

Pan

et al. 2018

Journal of Peptide Science

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“…Mating of immunized males with unvaccinated females showed reduction in the fertility. Interestingly, immunization with the above antigen did not lead to any histopathological changes in the testes or their function (112).…”

Section: Live-vector Based Contraceptive Vaccinesmentioning

confidence: 89%

“…Immunized animals showed reduction in fertility (111). Mimovirus expressing B cell epitope of spermatozoa specific protein, eppin, has also been developed (112). Intranasal immunization of male mice with the above mimovirus led to generation of anti-eppin IgA antibodies in the genital tract.…”

Section: Live-vector Based Contraceptive Vaccinesmentioning

confidence: 99%

Wildlife population management are contraceptive vaccines a feasible proposition

Gupta

Minhas

2017

Front Biosci

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“…ASAs as a vaccine needs to be at leastas effective than existing contraceptives in its properties and should be reversible [163,164,165,166,167,168,169]. The first attempt to evaluate the feasibility of using ASAs as a contraceptive in humans was made by Baskin et al who intramuscularly immunized a group of 20 fertile women with approximately 3 mL of freshly ejaculated semen [170].…”

Section: Role Of Asas In Designing Effective Contraceptionmentioning

confidence: 99%

Role of Antisperm Antibodies in Infertility, Pregnancy, and Potential for Contraceptive and Antifertility Vaccine Designs: Research Progress and Pioneering Vision

Vickram

Dhama

Chakraborty³

et al. 2019

Vaccines

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Sperm of humans, non-human primates, and other mammalian subjects is considered to be antigenic. The effect of changes in autoimmunity on reproductive cells such as spermatozoa and oocytes play a critical but indistinct role in fertility. Antisperm antibodies (ASAs) are invariably present in both females and males. However, the degree of ASA occurrence may vary according to individual and gender. Although the extent of infertility due to ASAs alone is yet to be determined, it has been found in almost 9–12% of patients who are infertile due to different causes. Postcoital presence of spermatozoa in the reproductive tract of women is not a contributory factor in ASA generation. However, ASA generation may be induced by trauma to the vaginal mucosa, or by anal or oral sex resulting in the deposition of sperm inside the digestive tract. It is strongly believed that, in humans and other species, at least some antibodies may bind to sperm antigens, causing infertility. This form of infertility is termed as immunological infertility, which may be accompanied by impairment of fertility, even in individuals with normozoospermia. Researchers target ASAs for two major reasons: (i) to elucidate the association between ASAs and infertility, the reason ASAs causes infertility, and the mechanism underlying ASA-mediated infertility; and (ii) to assess the potential of ASAs as a contraceptive in humans in case ASAs influences infertility. Therefore, this review explores the potential application of ASAs in the development of anti-spermatozoa vaccines for contraceptive purposes. The usefulness of ASAs for diagnosing obstructive azoospermia, salpingitis, and oligoasthenoteratozoospermia has been reviewed extensively. Important patents pertaining to potential candidates for spermatozoa-derived vaccines that may be utilized as contraceptives are discussed in depth. Antifertility vaccines, as well as treatments for ASA-related infertility, are also highlighted. This review will address many unresolved issues regarding mechanisms involving ASAs in the diagnosis, as well as prognoses, of male infertility. More documented scientific reports are cited to support the mechanisms underlying the potential role of ASA in infertility. The usefulness of sperm antigens or ASAs (recombinant) in human and wild or captive animal contraceptive vaccines has been revealed through research but is yet to be validated via clinical testing.

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Nasal immunization using a mimovirus vaccine based on the Eppin B-cell epitope induced suppressed fertility in mice

Cited by 4 publications

References 28 publications

ZNF185‐derived peptide induces fertility suppression in mice

ZNF185‐derived peptide induces fertility suppression in mice

Wildlife population management are contraceptive vaccines a feasible proposition

Role of Antisperm Antibodies in Infertility, Pregnancy, and Potential for Contraceptive and Antifertility Vaccine Designs: Research Progress and Pioneering Vision

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