Natribaculum breve gen. nov., sp. nov. and Natribaculum longum sp. nov., halophilic archaea isolated from saline soil

Liu, Qin; Min, Ren; Zhang, Li-Li

doi:10.1099/ijs.0.060541-0

Cited by 34 publications

(15 citation statements)

References 19 publications

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“…The highest levels of diversity were observed in sediment and water samples of relatively lower salinity, providing additional evidence for the emerging view that habitats of lower and fluctuating salinity are reservoirs for novel Halobacteria diversity (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). The use of 16S rRNA gene primers targeting members of the Halobacteria class for diversity and quantification studies is crucial for the targeted exploration of Halobacteria diversity in such habitats, since the prevailing levels of salinity and putative frequent salinity fluctuation are conducive to the coexistence of additional halotolerant and nonhalophilic microorganisms.…”

Section: Discussionmentioning

confidence: 92%

“…However, in environments with relatively lower salinity and/or fluctuating salinities, e.g., saline soils (salt plains and alpine salt sediments, soils adjacent to salt-processing plants), traditional Asian salted and fermented seafood products (e.g., jeotgal), and marine sponges, they usually coexist as a smaller fraction of the more diverse prokaryotic community inhabiting these settings (7)(8)(9)(10)(11)(12)(13). These habitats with moderate or low salinity and/or fluctuating salinity have been the source of species of many recently described novel Halobacteria taxa (14)(15)(16)(17)(18) and are partially responsible for the rapid expansion of recognized Halobacteria spp. during the last decade (19,20).…”

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confidence: 99%

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Patterns and Determinants of Halophilic Archaea (Class Halobacteria) Diversity in Tunisian Endorheic Salt Lakes and Sebkhet Systems

Najjari

Elshahed

Chérif

et al. 2015

Appl Environ Microbiol

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We examined the diversity and community structure of members of the halophilic Archaea (class Halobacteria) in samples from central and southern Tunisian endorheic salt lakes and sebkhet (also known as sebkha) systems using targeted 16S rRNA gene diversity survey and quantitative PCR (qPCR) approaches. Twenty-three different samples from four distinct locations exhibiting a wide range of salinities (2% to 37%) and physical characteristics (water, salt crust, sediment, and biofilm) were examined. A total of 4,759 operational taxonomic units at the 0.03 (species-level) cutoff (OTU 0.03 s) belonging to 45 currently recognized genera were identified, with 8 to 43 genera (average, 30) identified per sample. In spite of the large number of genera detected per sample, only a limited number (i.e., 2 to 16) usually constituted the majority (>80%) of encountered sequences. Halobacteria diversity showed a strong negative correlation to salinity (Pearson correlation coefficient ‫؍‬ ؊0.92), and community structure analysis identified salinity, rather than the location or physical characteristics of the sample, as the most important factor shaping the Halobacteria community structure. The relative abundance of genera capable of biosynthesis of the compatible solute(s) trehalose or 2-sulfotrehalose decreased with increasing salinities (Pearson correlation coefficient ‫؍‬ ؊0.80). Indeed, qPCR analysis demonstrated that the Halobacteria otsB (trehalose-6-phosphatase)/16S rRNA gene ratio decreases with increasing salinities (Pearson correlation coefficient ‫؍‬ ؊0.87). The results highlight patterns and determinants of Halobacteria diversity at a previously unexplored ecosystem and indicate that genera lacking trehalose biosynthetic capabilities are more adapted to growth in and colonization of hypersaline (>25% salt) ecosystems than trehalose producers.T he class Halobacteria represents a physiologically and phylogenetically distinct lineage within the archaeal phylum Euryarchaeota. Members of the Halobacteria are encountered in a wide range of environments where their absolute requirement for salt is satisfied. Within various hypersaline (Ͼ25% salt), thalassohaline (e.g., crystallizer ponds in solar salterns), and athalassohaline (e.g., the Dead Sea, hypersaline lakes, and soda lakes) water bodies, members of the Halobacteria represent the majority of the cellular biomass (1-6). However, in environments with relatively lower salinity and/or fluctuating salinities, e.g., saline soils (salt plains and alpine salt sediments, soils adjacent to salt-processing plants), traditional Asian salted and fermented seafood products (e.g., jeotgal), and marine sponges, they usually coexist as a smaller fraction of the more diverse prokaryotic community inhabiting these settings (7-13). These habitats with moderate or low salinity and/or fluctuating salinity have been the source of species of many recently described novel Halobacteria taxa (14-18) and are partially responsible for the rapid expansion of recognized Halobacteria spp. during the ...

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Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 99%

Patterns and Determinants of Halophilic Archaea (Class Halobacteria) Diversity in Tunisian Endorheic Salt Lakes and Sebkhet Systems

Najjari

Elshahed

Chérif

et al. 2015

Appl Environ Microbiol

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“…The final structure and function of a particular protein is determined by interactions between the core and surface Natribaculum longum 2.6 7-7.5 37 Saline soil, China [341] residues of a protein and the solvent in which the protein is dissolved [174,183]. Hydrophobic residues are pushed into the interior of the protein by entropy and hydrophilic residues remain on the surface and bind to water molecules to make a hydration layer around the protein, which causes surface tension.…”

Section: High-salt Adaptation Of Haloenzymesmentioning

confidence: 99%

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

et al. 2017

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Halophilic archaea, also referred to as haloarchaea, dominate hypersaline environments. To survive under such extreme conditions, haloarchaea and their enzymes have evolved to function optimally in environments with high salt concentrations and, sometimes, with extreme pH and temperatures. These features make haloarchaea attractive sources of a wide variety of biotechnological products, such as hydrolytic enzymes, with numerous potential applications in biotechnology. The unique trait of haloarchaeal enzymes, haloenzymes, to sustain activity under hypersaline conditions has extended the range of already-available biocatalysts and industrial processes in which high salt concentrations inhibit the activity of regular enzymes. In addition to their halostable properties, haloenzymes can also withstand other conditions such as extreme pH and temperature. In spite of these benefits, the industrial potential of these natural catalysts remains largely unexplored, with only a few characterized extracellular hydrolases. Because of the applied impact of haloarchaea and their specific ability to live in the presence of high salt concentrations, studies on their systematics have intensified in recent years, identifying many new genera and species. This review summarizes the current status of the haloarchaeal genera and species, and discusses the properties of haloenzymes and their potential industrial applications. SYSTEMATICS OF HALOPHILIC ARCHAEAHalophilic micro-organisms are found in the three domains of life, and are extremophiles living in hypersaline environments in the presence of salt (generally NaCl) as a specific requisite for their growth [1]. Hypersaline environments contain higher salt concentrations than seawater (3.5 % total dissolved salts) [2]. According to the optimum NaCl concentration for growth, micro-organisms fall into one of the following categories: extreme halophiles with optimum growth at 15-30 % (2.5-5.2 M) NaCl; moderate halophiles with optimum growth at 3-15 % (0.5-2.5 M) NaCl; slight halophiles with optimum growth at 1-3 % (0.2-0.5 M) NaCl; non-halophiles with optimum growth at less than 1 % (0.2 M) NaCl; and halotolerant micro-organisms which are non-halophiles showing the ability to tolerate high NaCl concentrations [3]. Halophiles can live in hypersaline environments because they are able to sustain osmotic balance. They use two main strategies to withstand the adverse consequences of water loss when exposed to high salt concentrations. The first strategy used by extremely halophilic archaea (haloarchaea) and halophilic anaerobic bacteria such as members of Halanaerobiales is known as the 'salt-in-cytoplasm' strategy, during which salts such as NaCl or KCl accumulate in the cytoplasm at concentrations similar to the extracellular ones [4,5]. Another mechanism to cope with high salt concentrations is the accumulation of organic molecules such as amino acids, sugars and polyols, known as compatible solutes or osmolytes. Compatible solutes are small and highly soluble molecules that can...

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“…Salt lakes are natural athalassohaline environments hosting diverse halophilic archaea, members of the family Halobacteriaceae within the order Halobacteriales [1,7,12,15,16]. The family Halobacteriaceae encompasses 47 genera containing over 165 species as of February 2014 [13,14].…”

Section: Introductionmentioning

confidence: 99%

Halorientalis brevis sp. nov., Isolated from an Inland Salt Lake of China

et al. 2015

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Halophilic archaeal strain YC89(T) was isolated from Yuncheng salt lake in Shanxi, China. Cells from strain YC89(T) were short rods, lysed in distilled water, stained Gram-negative and formed red-pigmented colonies on agar plate. Strain YC89(T) was able to grow at 25-50°C (optimum 37°C), at 1.4-4.8 M NaCl (optimum 2.6-3.1 M), at 0-1.0 M MgCl2 (optimum 0.3 M) and at pH 6.0-9.5 (optimum pH 7.5). The major polar lipids are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, sulfated mannosyl glucosyl diether and two unknown glycolipids. 16S rRNA gene analysis revealed that strain YC89(T) was phylogenetically related to Halorientalis persicus D108(T) (95.6% nucleotide identity) and H. regularis TNN28(T) (95.3% nucleotide identity). The rpoB' gene similarities between strain YC89(T) and H. persicus IBRC-M 10043(T) and H. regularis TNN28(T) were 88.1 and 88.0%, respectively. The DNA G+C content of strain YC89(T) was determined to be 61.3 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggested that strain YC89(T) (=CGMCC 1.12125(T) = JCM 18366(T)) represents a new species of Halorientalis, for which the name H. brevis sp. nov. is proposed.

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Natribaculum breve gen. nov., sp. nov. and Natribaculum longum sp. nov., halophilic archaea isolated from saline soil

Cited by 34 publications

References 19 publications

Patterns and Determinants of Halophilic Archaea (Class Halobacteria) Diversity in Tunisian Endorheic Salt Lakes and Sebkhet Systems

Patterns and Determinants of Halophilic Archaea (Class Halobacteria) Diversity in Tunisian Endorheic Salt Lakes and Sebkhet Systems

Systematics of haloarchaea and biotechnological potential of their hydrolytic enzymes

Halorientalis brevis sp. nov., Isolated from an Inland Salt Lake of China

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