Natural apoptosis in developing mice dopamine midbrain neurons and vermal Purkinje cells

Abstract

A b s t r a c tNatural cell death by apoptosis was studied in two neuronal populations of BALB/c, [11][12][12][13][13][14]

Caspase-3 Mediated Cell Death in the Normal Development of the Mammalian Cerebellum

“…However, after combined 3 H-thymidine autoradiography, TUNEL and active caspase-3 immunostaining it was clear that the PCD of the PNs was independent from their birth-date, i.e. it was not related to interactions with targets [109].…”

“…During subsequent maturation, the plate, which was originally composed of several layers of cells, becomes a monolayer of well aligned cells, a process that is completed within the first week after birth in rat and mouse and governed by Reelin, a glycoprotein of the extracellular [3], depended upon caspase-3 activation as e.g. 5.9% neurons in the Purkinje cell layer were immunostained using an antibody that recognized the cleaved form of the enzyme at P3 [81], and affected a higher number of PNs at P4 than P7 [109]. However, after combined 3 H-thymidine autoradiography, TUNEL and active caspase-3 immunostaining it was clear that the PCD of the PNs was independent from their birth-date, i.e.…”

Caspase-3 Mediated Cell Death in the Normal Development of the Mammalian Cerebellum

“…NOND affects the PNs in the course of their generation and migration with a plateau during the first postnatal week and is important for late compartmentation and wiring of the cerebellar cortex [105,106,107,108]. On ultrastructural bases, NOND was apoptotic [3], depended upon caspase-3 activation as, e.g., 5.9% neurons in the Purkinje cell layer were immunostained using an antibody that recognized the cleaved form of the enzyme at P3 [81], and affected a higher number of PNs at P4 than P7 [109]. However, after combined 3 H-thymidine autoradiography, TUNEL and active caspase-3 immunostaining it was clear that the PCD of the PNs was independent from their birth-date, i.e., it was not related to interactions with targets [109].…”

“…On ultrastructural bases, NOND was apoptotic [3], depended upon caspase-3 activation as, e.g., 5.9% neurons in the Purkinje cell layer were immunostained using an antibody that recognized the cleaved form of the enzyme at P3 [81], and affected a higher number of PNs at P4 than P7 [109]. However, after combined 3 H-thymidine autoradiography, TUNEL and active caspase-3 immunostaining it was clear that the PCD of the PNs was independent from their birth-date, i.e., it was not related to interactions with targets [109]. Immunocytochemical studies on the expression of the apoptosis-regulatory proteins Bid, Bcl-2, Bcl-X L , Bax and Bak during development of murine nervous system were supportive about the apoptotic nature of postnatal NOND of the PNs, as these neurons expressed high levels of Bid (pro-apoptotic) and Bcl-X L (anti-apoptotic) at P7 [110].…”

The Role Apoptosis in Violation of Functions Neurons

“…К сожалению, количество работ, проведённых на материале мозга и сетчатки человека, остаётся ограниченным [47,65,66], корреляционный анализ апоптоза в сетчатке и мозге практически отсутствует. Основная часть исследований выполнена на материале нервной ткани животных и на культурах различных клеток нейральной сетчатки [33,51,55,56], молекулярно-генетические исследования в большей степени проводятся на насекомых с последующей экстраполяцией на геном клеток человека [77]. [60].…”