Natural Composition and Biosynthetic Pathways of Alkaloids in Medicinal Dendrobium Species

Song, Cheng; Ma, Jingbo; Li, Guohui; Pan, Haoyu; Zhu, Yanfang; Jin, Qing; Cai, Yongping; Han, Bangxing

doi:10.3389/fpls.2022.850949

Cited by 12 publications

(19 citation statements)

References 138 publications

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“…D. huoshanense has attracted many researchers because of its broad pharmacological activity [ 9 ]. Our research group on D. huoshanense has carried out a series of studies on the field resource protection, tissue culture and cultivation, chemical component separation and identification, bioactivity analysis and action mechanism, and new drug and product development [ 3 , 5 , 10 , 39 , 50 , 51 , 52 , 53 ], and also completed its whole genome sequencing [ 54 ]. In addition, the transcriptome sequencing of D. huoshanense had been reported several times in other research groups [ 13 , 14 , 15 , 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…The research on the biosynthetic pathway mechanisms of the polysaccharides, alkaloids, and flavonoids of D. huoshanense is the main concern of researchers in this field. Although previous studies have described some candidate genes related to D. huoshanense [ 13 , 14 , 15 , 16 , 17 , 39 ], it is still necessary to find appropriate reference genes for further enhancing the detection of different gene expression levels of D. huoshanense under different experimental conditions by using qPCR.…”

Section: Introductionmentioning

confidence: 99%

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Selection of Suitable Reference Genes for Gene Expression Normalization Studies in Dendrobium huoshanense

Tian

et al. 2022

Genes

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Dendrobium huoshanense is a kind of precious herb with important medicinal and edible value in China, which is widely used in traditional Chinese medicine for various diseases. Recent studies have paid close attention to the genetic expression of the biosynthetic pathway of the main active components (polysaccharides, alkaloids, and flavonoids), and real-time polymerase chain reaction (qPCR) is one of the most widely used methods for doing so. However, so far, no reference gene selections have been reported in D. huoshanense. In this study, 15 reference gene candidates (GAPDH, eIF, EF-1α, PP2A, UBCE, RPL5, TBP, APT1, MDH, PTBP3, PEPC, CYP71, NCBP2, TIP41, and F-box) were selected and evaluated for their expression stability in D. huoshanense under various experimental conditions, including in different tissues (root, stem, and leaf), abiotic stresses (oxidative, drought, cold, and UV), and hormone treatment (methyl jasmonate) using three statistical programs (geNorm, NormFinder, and BestKeeper). Then, the RefFinder program was employed to comprehensively validate the stability of the selected reference genes. Finally, the expression profiles of the CESA and GMPP genes were further analyzed, and these results indicated that TBP, NCBP2, and CYP71 were the top three most stable reference genes after comprehensive comparison, which could be used as stable reference genes for normalizing the genes expression in D. huoshanense. This study described here provides the first data regarding on reference gene selection in D. huoshanense, which will be extremely beneficial for future research on the gene expression normalization in D. huoshanense.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selection of Suitable Reference Genes for Gene Expression Normalization Studies in Dendrobium huoshanense

Tian

et al. 2022

Genes

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“…D. officinale is a perennial herb in the orchid family that belongs to the Dendrobium genus ( Wang Y et al, 2021 ). It is mainly distributed in the Ta-pieh Mountain of Anhui, Zhengjiang, Guangzhou, Guangxi, Yunnan, and other provinces ( Song et al, 2022b ). D. officinale has considerable effects on immunity and hematopoiesis, as well as anti-oxidation, anti-tumor, anti-fatigue, and hypoglycemic properties ( Song et al, 2022d ).…”

Section: Introductionmentioning

confidence: 99%

In-depth analysis of large-scale screening of WRKY members based on genome-wide identification

Pan

Chen²,

Zhao³

et al. 2023

Front. Genet.

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With the rapid advancement of high-throughput sequencing technology, it is now possible to identify individual gene families from genomes on a large scale in order to study their functions. WRKY transcription factors are a key class of regulators that regulate plant growth and abiotic stresses. Here, a total of 74 WRKY genes were identified from Dendrobium officinale Kimura et Migo genome. Based on the genome-wide analysis, an in-depth analysis of gene structure and conserved motif was performed. The phylogenetic analysis indicated that DoWRKYs could be classified into three main groups: I, II, and III, with group II divided into five subgroups: II-a, II-b, II-c, II-d, and II-e. The sequence alignment indicated that these WRKY transcriptional factors contained a highly conserved WRKYGQK heptapeptide. The localization analysis of chromosomes showed that WRKY genes were irregularly distributed across several chromosomes of D. officinale. These genes comprised diverse patterns in both number and species, and there were certain distinguishing motifs among subfamilies. Moreover, the phylogenetic tree and chromosomal location results indicated that DoWRKYs may have undergone a widespread genome duplication event. Based on an evaluation of expression profiles, we proposed that DoWRKY5, 54, 57, 21, etc. may be involved in the transcriptional regulation of the JA signaling pathway. These results provide a scientific reference for the study of DoWRKY family genes.

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“…For the decades, dozens of alkaloids have been subsequently identified from D. anosmum, D. chrysanthum, D. crepidatum, D. findlayanum, D. friedricksianum, D. hilderbrandii, D. loddigessi, D. lohohense, D. moniliforme, D. parishii, D. pierardii, D. primulimun, D. snowflake and D. wardianum (Morita et al, 2000;Liu et al, 2007;Xu et al, 2013). The alkaloids identified from Dendrobiun plants could be divided into five categories based on their chemical structures, including sesquiterpenoid alkaloids, indolizidine alkaloids, pyrrolidine alkaloids, phthalide alkaloids, and imidazole alkaloids (Song et al, 2022b). Although different types of alkaloids have been isolated from many Dendrobium spp., the composition and biosynthesis of the alkaloids in D. officinale remain unsolved issues.…”

Section: Introductionmentioning

confidence: 99%

Identification of alkaloids and related intermediates of Dendrobium officinale by solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry

Song

Zhang²,

Manzoor³

et al. 2022

Front. Plant Sci.

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Jasmonate (JA) signaling plays a pivotal role in plant stress responses and secondary metabolism. Many studies have demonstrated that JA effectively induce the expressions of alkaloid biosynthetic genes in various plants, which rendered to the accumulation of alkaloid to counteract stresses. Despite the multiple roles of JA in the regulation of plant growth and different stresses, less studied involved in the regulatory role of JA in Dendrobium officinale alkaloids. A strategy for the rapid identification of alkaloid and the intermediates of D. officinale was established based on a solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry method. By using SPE-LC-MS/MS method, the potential compounds were tentatively identified by aligning the accurate molecular weight with the METLIN and Dictionary of Natural Products databases. The chemical structures and main characteristic fragments of the potential compounds were further confirmed by retrieving the multistage mass spectra from the MassBank and METLIN databases. The Mass Frontier software was used to speculate the fragmentation pathway of the identified compounds. Seven alkaloids were separated and identified from D. officinale, which were mainly classified into five types (tropane alkaloids, tetrahydroisoquinoline alkaloids, quinolizidine alkaloids, piperidine alkaloids, and spermidine alkaloids). Besides the alkaloids, forty-nine chemical substances, including guanidines, nucleotides, dipeptides, sphingolipids and nitrogen-containing glucosides, were concurrently identified. These findings gives the composition of chemicals currently found in D. officinale, which could provide the scientific method for the identification of alkaloids in other Dendrobium plants.

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Natural Composition and Biosynthetic Pathways of Alkaloids in Medicinal Dendrobium Species

Cited by 12 publications

References 138 publications

Selection of Suitable Reference Genes for Gene Expression Normalization Studies in Dendrobium huoshanense

Selection of Suitable Reference Genes for Gene Expression Normalization Studies in Dendrobium huoshanense

In-depth analysis of large-scale screening of WRKY members based on genome-wide identification

Identification of alkaloids and related intermediates of Dendrobium officinale by solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry

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