Natural deep eutectic solvents as a biocompatible tool for the extraction of blueberry anthocyanins

Silva, Dariane Trivisiol da; Pauletto, Roberson; Cavalheiro, Sabrina da Silva; Bochi, Vivian Caetano; Rodrigues, Eliseu; Wéber, Júlia; Silva, Cristiane de Bona da; Morisso, Fernando Dal Pont; Barcia, Milene Teixeira; Emanuelli, Tatiana

doi:10.1016/j.jfca.2020.103470

Cited by 90 publications

(36 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This effect might be because the addition of water effectively weakened the hydrogen bonding of the DESs [ 31 ], resulting in a rapid decrease in viscosity, which was conducive to improving the solubility and the mass transfer rate of the anthocyanins. Nevertheless, the anthocyanin yields observably decreased when the water content continuously increased from 30% to 50% ( p < 0.05), which was because the excessively high water content increased the DESs polarity and decreased the solubility of anthocyanins in the DESs [ 32 ]. Thus, 20%, 30% and 40% water contents were employed for the subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

Xue

Tan

et al. 2020

Foods

View full text Add to dashboard Cite

Raspberries have been reported to contain abundant anthocyanins and other active compounds. To extract anthocyanins from raspberries more efficiently, a novel procedure of ultrasound-assisted deep eutectic solvent extraction (UADESE) was proposed in this paper. The extraction process was optimized by response surface methodology coupled with a genetic algorithm. The optimum extraction parameters to achieve the highest yield of anthocyanins 1.378 ± 0.009 mg/g from raspberry powder via UADESE were obtained at a water content of 29%, ultrasonic power of 210 W, extraction temperature of 51 °C and extraction time of 32 min. The AB-8 macroporous resin combined with the high-speed counter current chromatography (HSCCC) method were further used to isolate and purify the anthocyanins extracts obtained under optimum extraction conditions, and the structure of purified anthocyanins components were identified by UV-Visible spectrophotometer (UV-Vis), high-performance liquid chromatography (HPLC), high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS/MS), 1H nuclear magnetic resonance (NMR) and 13C-NMR spectra. The two anthocyanins (cyanidin-3-glucoside with a purity of 92.25% and cyanidin-3-rutinoside with a purity of 93.07%) identified were consistent with those present in raspberries. These findings provided an effective and feasible method for extraction, isolation and purification of anthocyanins from natural plant resources.

show abstract

Section: Resultsmentioning

confidence: 99%

Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

Xue

Tan

et al. 2020

Foods

View full text Add to dashboard Cite

show abstract

“…Several methods were optimized for NADES-based anthocyanin extraction with the same efficacy of conventional organic solvent but with better yields compared to an exhaustive extraction with the organic solvent. NADES represent an excellent, useful updated strategy for green extraction and biocompatible preparation of anthocyanin-based products for pharmaceutical and nutraceutical applications [ 41 , 42 , 43 , 44 ].…”

Section: Chemistry and Biochemistry Of Anthocyaninsmentioning

confidence: 99%

“…Glycosides also are absorbed by the small intestine, possibly by the sodium-dependent glucose transporter SGLT1. Acylated anthocyanins also are absorbed, but the amount is 4-fold lower than that of non-acylated anthocyanins [ 44 , 93 ].…”

Section: Anthocyanins In Foodmentioning

confidence: 99%

Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases

et al. 2020

View full text Add to dashboard Cite

Anthocyanins are a class of water-soluble flavonoids widely present in fruits and vegetables. Dietary sources of anthocyanins include red and purple berries, grapes, apples, plums, cabbage, or foods containing high levels of natural colorants. Cyanidin, delphinidin, malvidin, peonidin, petunidin, and pelargonidin are the six common anthocyanidins. Following consumption, anthocyanin, absorption occurs along the gastrointestinal tract, the distal lower bowel being the place where most of the absorption and metabolism occurs. In the intestine, anthocyanins first undergo extensive microbial catabolism followed by absorption and human phase II metabolism. This produces hybrid microbial–human metabolites which are absorbed and subsequently increase the bioavailability of anthocyanins. Health benefits of anthocyanins have been widely described, especially in the prevention of diseases associated with oxidative stress, such as cardiovascular and neurodegenerative diseases. Furthermore, recent evidence suggests that health-promoting effects attributed to anthocyanins may also be related to modulation of gut microbiota. In this paper we attempt to provide a comprehensive view of the state-of-the-art literature on anthocyanins, summarizing recent findings on their chemistry, biosynthesis, nutritional value and on their effects on human health.

show abstract

“…This method is often preferred for the extraction of heat-sensitive compounds such as anthocyanins [ 12 , 13 ]. The principal advantages of NADES are that their interaction confer excellent physical and chemical properties, such as low volatility, nonflammability, and adjustable viscosity [ 10 , 14 , 15 ], giving NADES solubilizing behavior that is different from other conventional solvents and has been used more recently in combination with ultrasound energy in order to increase extraction yield of natural products [ 16 , 17 , 18 , 19 , 20 ]. In particular, it has been seen that the use of NADES composed of combinations of quaternary ammonium salt-alcohol, quaternary ammonium salt-carboxylic acid and carboxylic acid-sugar have been used to extract anthocyanins from plant matrices.…”

Section: Introductionmentioning

confidence: 99%

“…In particular, it has been seen that the use of NADES composed of combinations of quaternary ammonium salt-alcohol, quaternary ammonium salt-carboxylic acid and carboxylic acid-sugar have been used to extract anthocyanins from plant matrices. The most widely used, both in binary and ternary combinations, are citric acid, mal acid, lactic acid, glucose, fructose, xylose, glycerol and choline chloride [ 13 , 19 , 21 , 22 , 23 ]. An important point is that in most of the jobs UAE is used as an extraction method because it has been proven that it has the advantage of being green, fast and clean, which improves the yield of anthocyanin extraction [ 13 , 19 , 22 , 23 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-Assisted Extraction of Anthocyanins Using Natural Deep Eutectic Solvents and Their Incorporation in Edible Films

et al. 2021

View full text Add to dashboard Cite

Extracts rich in bioactive compounds added to edible films have allowed the development of active packaging that increases the shelf life of food. However, it is necessary to search for solvents that are nontoxic and not harmful to the environment, with natural deep eutectic solvents (NADES) being an attractive and easily synthesized alternative. This research aimed to design NADES by lyophilization to be used in the extraction of anthocyanins from the Chilean Luma chequen (Molina) A. Gray berry, and subsequently adding them to the matrix of edible ƙ-carrageenan films. For this purpose, ultrasound-assisted extraction (UAE) was used and the anthocyanin content was evaluated with the pH differential method. The antioxidant capacity of extracts was determined by DPPH assay and the antibacterial capacity by diffusion agar tests. The results obtained indicate that the designed NADES are efficient at extracting anthocyanins, reaching concentrations between 81.1 and 327.6 mg eq cyanidin 3-glucoside/100 g dw of L. chequen (Molina) A. Gray. The extracts reached inhibition diameters between 5 and 34 mm against Escherichia coli, Staphylococcus aureus, and Salmonella typhi strains. Once the extracts were incorporated into ƙ-carrageenan films, active edible films with antioxidant and antibacterial capacities were obtained.

show abstract

Natural deep eutectic solvents as a biocompatible tool for the extraction of blueberry anthocyanins

Cited by 90 publications

References 38 publications

Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

Optimization Ultrasound-Assisted Deep Eutectic Solvent Extraction of Anthocyanins from Raspberry Using Response Surface Methodology Coupled with Genetic Algorithm

Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases

Ultrasound-Assisted Extraction of Anthocyanins Using Natural Deep Eutectic Solvents and Their Incorporation in Edible Films

Contact Info

Product

Resources

About