Natural diarylheptanoid compounds from Curcuma comosa Roxb. promote differentiation of mouse myoblasts C2C12 cells selectively via ER alpha receptors

Tipbunjong, Chittipong; Kitiyanant, Yindee; Chaturapanich, Ganyapong; Sornkaew, Nilubon; Suksamrarn, Apichart; Kitiyanant, Narisorn; Esser, Karyn A.; Pholpramool, Chumpol

doi:10.1007/s00044-016-1748-y

Cited by 3 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this respect, experiments on C2C12 cells treated with diarylheptanoid compounds (HPPH) provide very important information (Tipbunjong et al 2017(Tipbunjong et al , 2019. These molecules display growth-and differentiationpromoting effects on C2C12 cells that involve a membrane form of ERα receptor bound by a palmitoyl anchor, and they were abolished by 2-bromohexadecanoic acid, an inhibitor of palmitoylation (Tipbunjong et al 2019).…”

Section: Discussionmentioning

confidence: 99%

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

Lafont

Serova

Didry-Barca

et al. 2022

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

20-Hydroxyecdysone (20E) is a steroid hormone that plays a key role in insect development through nuclear ecdysteroid receptors (EcR/RXR complex) and at least one membrane GPCR receptor (DopEcR). It also displays numerous pharmacological effects in mammals, where its mechanism of action is still debated, involving either an unidentified GPCR or the estrogen ERβ receptor. The goal of this study was to better understand 20E mechanism of action in mammals. A mouse myoblast cell line (C2C12) and the gene expression of myostatin (a negative regulator of muscle growth) was used as a reporter system of anabolic activity. Experiments using protein-bound 20E established the involvement of a membrane receptor. 20E-like effects were also observed with angiotensin-(1-7), the endogenous ligand of Mas. Additionally, the effect on myostatin gene expression was abolished by Mas receptor knock-down using small interfering RNA (siRNA) or pharmacological inhibitors. 17β-Estradiol (E2) also inhibited myostatin gene expression, but protein-bound E2 was inactive, and E2 activity was not abolished by angiotensin-(1-7) antagonists. A mechanism involving cooperation between the Mas receptor and a membrane-bound palmitoylated estrogen receptor is proposed. The possibility to activate the Mas receptor with a safe steroid molecule is consistent with the pleiotropic pharmacological effects of ecdysteroids in mammals and, indeed, the proposed mechanism may explain the close similarity between angiotensin-(1-7)’s and 20E’s effects. Our findings open up many possible therapeutic developments involving stimulation of the protective arm of the renin-angiotensin-aldosterone system (RAAS) with 20E.

show abstract

Section: Discussionmentioning

confidence: 99%

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

Lafont

Serova

Didry-Barca

et al. 2022

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

show abstract

“…Multiple hydroxyls are favorable for the activity, while mono-and di-hydroxyls exhibit no detectable activity [19]. [12]. These compounds also have been shown to promote proliferation in MC3T3-E1 mouse pre-osteoblast [15] and human osteoblast cells [16].…”

Section: Discussionmentioning

confidence: 99%

“…Other diarylheptanoids from different plant species also exhibit a protective effects on cell death induced by different chemicals. The action of curcuminoids on myoblast differentiation is abolished by ICI 182,780, suggesting that curcuminoids enhance myoblast differentiation by activating ER [12]. In addition, curcuminoids also mediate their activity through the ER to enhance differentiation in osteoblasts [15,16].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Synthetic curcuminoid analogues abrogate oxidationinduced cell death and promote myogenic differentiation of C2C12 mouse myoblasts

Tipbunjong¹,

Sookbangnop²,

Ajavakom³

et al. 2018

Trop. J. Pharm Res

Self Cite

View full text Add to dashboard Cite

Purpose: To investigate the ability of two synthetic curcuminoid analogues, 6-(4-hydroxy-3methoxyphenethyl)-5-(3-(4-hydroxy-3-methoxyphenyl)propanoyl)-4-phenyl-3,4-dihydropyrimidin-2(1H)one (compound A) and 6-(4-hydroxy-3-methoxyphenethyl)-4-(4-hydroxy-3-methoxyphenyl)-5-(3-(4hydroxy-3-methoxyphenyl)propanoyl)-3,4-dihydropyrimidin-2(1H)-one (compound B), to protect against oxidation-induced cell death and the potential to enhance proliferation and differentiation of C2C12 myoblast cells. Methods: Antioxidant activity of curcuminoid analogues was evaluated by DPPH assay. The cytotoxic activity of the compounds (0-25 mM) on C2C12 myoblasts was determined by MTT assay while the effect on cell proliferation was assessed by BrdU uptake. Myoblast cell differentiation was measured by the formation of myotubes and myosin heavy chain (MHC) protein expression using immunofluorescence staining and Western blotting, respectively. Results: Both curcuminoid analogues exhibited strong anti-oxidant activity of up to 3-fold greater than that of ascorbic acid, and were non-toxic to C2C12 myoblasts at concentrations up to 25 mM. Furthermore, these curcuminoid analogues mitigated myoblast cell death induced by oxidative stress. Notably, both analogues (10 nM) had no effect on cell proliferation. However, only compound A significantly enhanced myoblast differentiation comparable to the effects of dihydrotestosterone (1 µM) and estradiol (10 nM). Conclusion: The results suggest that compound A may serve as a lead compound for the development of suitable therapeutic agents for muscle injuries and diseases.

show abstract

Diarylheptanoid 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one enhances C2C12 myoblast differentiation by targeting membrane estrogen receptors and activates Akt-mTOR and p38 MAPK-NF-κB signaling axes

et al. 2019

View full text Add to dashboard Cite

Natural diarylheptanoid compounds from Curcuma comosa Roxb. promote differentiation of mouse myoblasts C2C12 cells selectively via ER alpha receptors

Cited by 3 publications

References 40 publications

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

20-Hydroxyecdysone activates the protective arm of the RAAS via the MAS receptor

Synthetic curcuminoid analogues abrogate oxidationinduced cell death and promote myogenic differentiation of C2C12 mouse myoblasts

Diarylheptanoid 1-(4-hydroxyphenyl)-7-phenyl-(6E)-6-hepten-3-one enhances C2C12 myoblast differentiation by targeting membrane estrogen receptors and activates Akt-mTOR and p38 MAPK-NF-κB signaling axes

Contact Info

Product

Resources

About