Natural killer cell mediated cytotoxicity of tumor cells initiated by neem leaf preparation is associated with CD40–CD40L–mediated endogenous production of interleukin-12

Bose, Anamika; Baral, Rathindranath

doi:10.1016/j.humimm.2007.08.002

Cited by 39 publications

(35 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to further study the role of NLGP in restoring several immune dysfunctions, 7,9,26 we evaluated the regulatory role of this immunomodulator on dysregulated CCR5 signaling in HNSCC patient cells. This study is particularly relevant because of a report on CXC chemokines in HNSCC, 7 in which a positive role for NLGP was demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…22 In addition, we have also observed p38 MAPK activation in monocytes after NLGP treatment. 9 Thus, we examined p38 MAPK activation in monocyte signaling to determine whether it was involved in NLGP-induced CCR5-dependent monocyte chemotaxis. To this end, purified MO/Mw from HNSCC patients (n53) and healthy individuals (n53) were treated with NLGP for 4 h and western blot analysis was performed with cellular lysates.…”

Section: Nlgp-induced Monocyte Chemotaxis Is Partially Dependent On Pmentioning

confidence: 99%

“…One such function is antigen presentation and positive costimulation, which is mainly performed by antigen presenting cells. These crucial cellular functions are compromised by different negative stimuli in head and neck squamous cell carcinoma (HNSCC) patients, 8,9 and we hypothesize that their suboptimal function can be modulated by NLGP. 9,10 In the present communication, we examined the migration of MO/Mw towards tumors by optimal utilization of chemokine signaling to maximize T cell-mediated tumor cell killing.…”

Section: Introductionmentioning

confidence: 99%

“…These crucial cellular functions are compromised by different negative stimuli in head and neck squamous cell carcinoma (HNSCC) patients, 8,9 and we hypothesize that their suboptimal function can be modulated by NLGP. 9,10 In the present communication, we examined the migration of MO/Mw towards tumors by optimal utilization of chemokine signaling to maximize T cell-mediated tumor cell killing. In the migratory process of MO/ Mw, CC chemokines, such as MIP-1a, MIP-1b and RANTES, may play crucial roles through binding to their receptor, CCR5.…”

Section: Introductionmentioning

confidence: 99%

“…11 Although CCR5 is expressed normally in many immune cells, such as lymphocytes, in MO/Mw chemotaxis and in assisting effector T cells to induce tumor cell cytotoxicity. In addition, previous data showing a positive impact of NLGP on macrophage function 9,17 prompted us to examine the role of NLGP in modulation of CCR5 ligand (RANTES, MIP-1a and MIP-1b) imbalance that may induce successful migration of monocytes towards a tumor. Specifically, we examined the role of chemokine modulation of monocytes by NLGP in the induction of T cell-mediated tumor cell cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Restoration of dysregulated CC chemokine signaling for monocyte/macrophage chemotaxis in head and neck squamous cell carcinoma patients by neem leaf glycoprotein maximizes tumor cell cytotoxicity

et al. 2010

Self Cite

View full text Add to dashboard Cite

Previous studies have shown that the CC chemokine receptor CCR5 is downregulated on monocyte/macrophage (MO/Mw) surfaces in head and neck squamous cell carcinoma (HNSCC) patients (stage IIIB). Ligands (RANTES, MIP-1a and MIP-1b) of this chemokine receptor were also secreted in lesser quantity from MO/Mw of HNSCC patients in comparison with healthy individuals. In an aim to restore this dysregulated receptor-ligand signaling, we have used neem leaf glycoprotein (NLGP), a novel immunomodulator reported from our laboratory. NLGP upregulated CCR5 expression, as evidenced from studies on MO/Mw of peripheral blood from HNSCC patients as well as healthy individuals. Expression of RANTES, MIP-1a and MIP-1b was also upregulated following NLGP treatment of these cells in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Nlgp-induced Monocyte Chemotaxis Is Partially Dependent On Pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Restoration of dysregulated CC chemokine signaling for monocyte/macrophage chemotaxis in head and neck squamous cell carcinoma patients by neem leaf glycoprotein maximizes tumor cell cytotoxicity

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

Chemopreventive activity of Azadirachta indica on two‐stage skin carcinogenesis in murine model

Arora

Koul

Bansal

2010

Phytotherapy Research

View full text Add to dashboard Cite

The present study reports the chemopreventive activity of aqueous Azadirachta indica leaf extract (AAILE) in a murine two-stage skin carcinogenesis model. Skin tumors were induced by topical application of 7,12-dimethylbenz(a)anthracene (DMBA) (500 nmol/100 µL for 2 weeks) followed by 12-O-tetradecanoylphorbol-13-acetate (TPA) (1.7 nmol/100 µL of acetone, twice weekly) as a promoter. Male LACA mice were divided into four groups: control, DMBA/TPA, AAILE and AAILE + DMBA/TPA. AAILE was administered orally at a dose level of 300 mg/kg body weight thrice a week for 20 weeks. 100% tumor incidence was observed in the DMBA/TPA treated animals, whereas the AAILE + DMBA treated animals exhibited a tumor incidence of 58.3% only. A significant reduction in the mean tumor burden (54.5%) and mean tumor volume (45.6%) was observed in the mice that received AAILE along with DMBA/TPA. Topical application of DMBA/TPA to the skin resulted in well-developed carcinomas associated with decreased expression of pro-apoptotic protein such as caspase 3 and enhanced expression of antiapoptotic protein such as bcl-2 when compared with the control counterparts. However, adminstration of AAILE inhibited skin carcinogenesis with induction of pro-apoptotic proteins such as bax, caspase 3, caspase 9 and inhibition of antiapoptotic proteins such as bcl-2. These results suggest that the induction of apoptosis may be one of the mechanisms underlying the chemopreventive effects of A. indica.

show abstract

Food-Based Interventions for Cancer Management: An Ayurvedic Perspective

Pal

2013

Ayurvedic Science of Food and Nutrition

View full text Add to dashboard Cite

Natural killer cell mediated cytotoxicity of tumor cells initiated by neem leaf preparation is associated with CD40–CD40L–mediated endogenous production of interleukin-12

Cited by 39 publications

References 26 publications

Restoration of dysregulated CC chemokine signaling for monocyte/macrophage chemotaxis in head and neck squamous cell carcinoma patients by neem leaf glycoprotein maximizes tumor cell cytotoxicity

Restoration of dysregulated CC chemokine signaling for monocyte/macrophage chemotaxis in head and neck squamous cell carcinoma patients by neem leaf glycoprotein maximizes tumor cell cytotoxicity

Chemopreventive activity of Azadirachta indica on two‐stage skin carcinogenesis in murine model

Food-Based Interventions for Cancer Management: An Ayurvedic Perspective

Contact Info

Product

Resources

About