Natural material adsorbed onto a polymer to enhance immune function

Reinaque, Ana Paula Barcelos; França, Eduardo Luzía; Scherer, Edson Fredulin; Côrtes, Mayra Aparecida; Souto, Francisco José Dutra; Honório-França, Adenilda Cristina

doi:10.2147/dddt.s34622

Cited by 10 publications

(1 citation statement)

References 41 publications

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“…Several candidate functional materials have been evaluated by assessing the biocompatibility and interaction with human cells. 14,26 The data suggest that the acid and cement treatments applied to the metal surfaces resulted in a material with low cellular toxicity, which can be used to improve the application of cemented implant prostheses.…”

Section: Discussionmentioning

confidence: 99%

Dental implant surfaces treated with phosphoric acid can modulate cytokine production by blood MN cells

et al. 2019

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The study characterizes dental implant surfaces treated with phosphoric acid to assess the effects of acid treatment on blood cells and correlate them with cytokine levels. The implant surfaces examined were divided into untreated metal surface (US; n = 50), metal surface treated with phosphoric acid (ATS; n = 50) and cement surface (CS; n = 50) groups. The samples were characterized by scanning electron microscopy (SEM) and rheometry. The implants were incubated with human blood mononuclear cells for 24 h, with surface rinsing in the ATS treatment. Cell viability was determined by colorimetric methods and cytokines in the culture supernatant were quantified using flow cytometry. In the ATS group, the surface porosity and contact surface were increased and plaques were observed on the surface. The blood flow and viscosity curves were similar among the treatments, and the high cell viability rates indicate the biocompatibility of the materials used. An increase in the levels of IL-2, IL-4, IL-6, IL-10 and TNF-α was observed in the ATS and CS groups. There were positive correlations between IL-10 and IL-2 levels and between IL-10 and IL-4 levels in the culture supernatant of the ATS group. The results suggest that implant surface treatment with phosphoric acid activates the production of inflammatory cytokines. The increased cytokine levels can modulate the immune response, thereby improving biofunctional processes and promoting the success of dental implants.

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Section: Discussionmentioning

confidence: 99%

Dental implant surfaces treated with phosphoric acid can modulate cytokine production by blood MN cells

et al. 2019

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Extraction and determination of the Pimelea toxin simplexin in complex plant-polymer biocomposites using ultrahigh-performance liquid chromatography coupled with quadrupole Orbitrap mass spectrometry

et al. 2021

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The identification of signature genes and their relationship with immune cell infiltration in age-related macular degeneration

Chen,

Zhao,

Zhang

et al. 2024

Mol Biol Rep

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BackgroundAge-related macular degeneration (AMD) is a prevalent source of visual impairment among the elderly population, and its incidence has risen in tandem with the increasing longevity of humans. Despite the progress made in anti-VEGF therapy, the clinical outcomes have proven to be unsatisfactory. MethodWe obtained differentially expressed genes (DEGs) of AMD patients and healthy controls from GEO database. GO analysis and KEGG analysis were used to enrich the differential genes. Weighted gene coexpression network analysis (WCGNA) is used to screen modules related to AMD expression. SVM, random forest, and least absolute shrinkage and selection operator (LASSO) were used to screen hub gene. Gene set enrichment analysis (GSEA) is used to explore the pathway through which these hub genes are enriched. CIBERSORT was used to analyze the relationship between hub gene and immune cell in ltration. Finally, West blotting and RT-PCR were used to explore the expression of Hub gene in AMD mice. ResultsWe screened 1084 differential genes in GSE29801, of which 496 genes were up-regulated. 1084 differential genes were introduced into WCGNA analysis, and 94 genes related to AMD were obtained. 79 overlapping genes were obtained by VEEN plot. The 79 genes were introduced into three machine learning methods to screen the Hub gene, and the gene screened by the three methods was TNC,FAP,SREBF1,and TGF-β2. We veri ed their diagnostic function in GSE29801 and GSE103060 gene sets respectively. Then the pathway of hub gene co-enrichment was obtained by GO analysis and KEGG analysis. CIBERSORT analysis showed that these hub genes were associated with immune cell in ltration. Finally, we found increased expression of TNC, FAP, SREBF1, and TGF-β2 mRNA and protein in the retina of AMD mice. ConclusionWe found that four hub genes, FAP, TGF-β2 and SREBF1,and TNC, have diagnostic signi cance in patients with AMD and are related to immune cell in ltration. Finally, we found up-regulated of these hub genes mRNA and protein in the retina of AMD mice.

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Natural material adsorbed onto a polymer to enhance immune function

Cited by 10 publications

References 41 publications

Dental implant surfaces treated with phosphoric acid can modulate cytokine production by blood MN cells

Dental implant surfaces treated with phosphoric acid can modulate cytokine production by blood MN cells

Extraction and determination of the Pimelea toxin simplexin in complex plant-polymer biocomposites using ultrahigh-performance liquid chromatography coupled with quadrupole Orbitrap mass spectrometry

The identification of signature genes and their relationship with immune cell infiltration in age-related macular degeneration

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