Natural products and polysorbates: Potential Inhibitors of biofilm formation in Pseudomonas aeruginosa

Abstract

Introduction: With all the challenges super bugs are imposing, biofilm formation opens the door against various more complicated challenges. Such issue may be highlighted with the ability of the latter to render the antibiotics hardly accessible to bacterial cells and sheds the light on the importance of finding antibiofilm formers. Therefore, we assessed the inhibitory effect of natural product extracts (ginger, wild blueberry) and polysorbates (PS20, PS80) on biofilm formation at the molecular level.
 Methodology: Growth inhibition assay was performed to test the effect of ginger (Zingiber Officinale), wild blueberry (Vaccinium Angustifolium), and polysorbates on Pseudomonas aeruginosa (PAN14) growth. Transcription levels of biofilm exopolysaccharides encoding genes (ndvB, pelC, algC) and quorum sensing genes (lasI, lasR, rhlI, rhlR) for LasI/LasR and RhlI/ RhlR systems were evaluated by RT qPCR.
 Results: The polysorbates and the extracts of both ginger and wild blueberry had no effect on the growth of P. aeruginosa. Biofilms’ examination has unraveled the effectiveness of treatments used in reducing its formation. Moreover, a significant reduction in the expression of all genes tested for biofilm exopolysaccharides and its quorum sensing system was observed.
 Conclusion: The decrease in the relative gene expression of the exopolysaccharides and quorum sensing encoding genes sheds the light on the mechanism of action of ginger and wild blueberry’s constituents as well as polysorbates 20 and 80 on P. aeruginosa biofilm formation. Future studies need to assess the antibiofilm effect of each fraction of herbal extracts separately.

In Vitro Antibacterial, Anti-Adhesive and Anti-Biofilm Activities of Krameria lappacea (Dombey) Burdet & B.B. Simpson Root Extract against Methicillin-Resistant Staphylococcus aureus Strains

“…Interestingly, the treatment with 256.00 µg/mL of KLRE showed an inhibitory effect on both the biofilm formation as well as the metabolic activity of standard and MRSA strains. These findings are consistent with other studies that have proved the efficacy of natural products in inhibiting the development of biofilm [ 42 , 43 , 44 ]. Concerning that, in our previous paper, we demonstrated the suppressive action of walnut ( Juglans regia ) pellicle extract on growth, biofilm formation, and cell viability of coagulase-negative staphylococci (CoNS) [ 21 ].…”

Effects of daphnetin on biofilm formation and motility of pseudomonas aeruginosa

“…Natural products have recently attracted increased attention due to their safety and antibiofilm effects. Recent studies have shown that gingerols, proanthocyanins, and condensed tannins, which are extracted from Ginger (Zingiber Officinale) and wild blueberry (Vaccinium Angustifolium), have antibiofilm effects (Miari et al, 2020). Topa et al showed that Cinnamaldehyde could disrupt biofilm formation and the swarming motility of P. aeruginosa (Topa et al, 2018).…”

Antibacterial and antifungal activities herbácia Zingiber Officinale in dentistry: a literature review

“…Both compounds (crude extraction or methanolic fraction) interfere with hydrophobicity, between dental surface-bacterial interactions, through flavonoids, which reduce the enzyme GTFase and AG I / II, during synthesis / adherence, mainly of insoluble and dependent Glucans sucrose. Furthermore, the sites of bacterial binding, pH and integrity of exopolysaccharides are disturbed, in the formation of the climax community, disrupting pathogenicity and the sensing quorum (functioning as an anticariogenic), in addition to the expression of bacterial genes (Hasan et al, 2015;Miari et al, 2020).…”