Near-Infrared Sentinel Lymph Node Identification in Non–Small Cell Lung Cancer

Digesu, Christopher S.; Weiss, Kathleen D.; Colson, Yolonda L.

doi:10.1001/jamasurg.2017.5928

Cited by 7 publications

(2 citation statements)

References 8 publications

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“…Non-small cell lung cancer is a malignant tumor that accounts for 80% of lung cancers, the diagnosis of which is mostly made in late stages, resulting in a low number of colony formation and SFs ( Digesu et al, 2018 ). Moreover, the emergence of chemo- or radioresistance in numerous NSCLC cases has made the current therapeutic options yield unsatisfactory outcome ( Yin et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Overcoming of Radioresistance in Non-small Cell Lung Cancer by microRNA-320a Through HIF1α-Suppression Mediated Methylation of PTEN

Yuan

et al. 2020

Front. Cell Dev. Biol.

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Background Radioresistance is a major challenge in the use of radiotherapy for the treatment of lung cancer while microRNAs (miRs) have been reported to participate in multiple essential cellular processes including radiosensitization. This study was conducted with the main objective of investigating the potential role of miR-320a in radioresistance of non-small cell lung cancer (NSCLC) via the possible mechanism related to HIF1α, KDM5B, and PTEN. Methods Firstly, NSCLC radiosensitivity-related microarray dataset GSE112374 was obtained. Then, the expression of miR-320a, HIF1α, KDM5B, and PTEN was detected in the collected clinical NSCLC samples, followed by Pearson’s correlation analysis. Subsequently, ChIP assay was conducted to determine the content of the PTEN promoter fragment enriched by the IgG antibody and H3K4me3 antibody. Finally, a series of in vitro and in vivo assays were performed in order to evaluate the effects of miR-320a on radioresistance of NSCLC with the involvement of HIF1α, KDM5B, and PTEN. Results The microarray dataset GSE112374 presented with a high expression of miR-320a in NSCLC radiosensitivity samples, which was further confirmed in our clinical samples with the use of reverse transcription-quantitative polymerase chain reaction. Moreover, miR-320a negatively targeted HIF1α, inhibiting radioresistance of NSCLC. Interestingly, miR-320a suppressed the expression of KDM5B, and KDM5B was found to enhance the radioresistance of NSCLC through the downregulation of PTEN expression. The inhibition of miR-320a in radioresistance of NSCLC was also reproduced by in vivo assay. Conclusion Taken together, our findings were suggestive of the inhibitory effect of miR-320a on radioresistance of NSCLC through HIF1α-suppression mediated methylation of PTEN.

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Section: Discussionmentioning

confidence: 99%

Overcoming of Radioresistance in Non-small Cell Lung Cancer by microRNA-320a Through HIF1α-Suppression Mediated Methylation of PTEN

Yuan

et al. 2020

Front. Cell Dev. Biol.

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“…It is also more difficult to map with vital blue dyes due to the anthracotic nature of pulmonary lymph nodes. Recent series using fluorescent dyes and near infrared imaging have demonstrated outstanding survival among patients who are determined to be free of nodal metastases through SLN biopsy [ 55 ].…”

Section: Application Of Sentinel Lymph Node Surgery To Different Type...mentioning

confidence: 99%

The lymphatic system and sentinel lymph nodes: conduit for cancer metastasis

Leong

Pissas

Scarato

et al. 2021

Clin Exp Metastasis

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The lymphatic system is a complicated system consisting of the lymphatic vessels and lymph nodes draining the extracellular fluid containing cellular debris, excess water and toxins to the circulatory system. The lymph nodes serve as a filter, thus, when the lymph fluid returns to the heart, it is completely sterile. In addition, the lymphatic system includes the mucosa-associated lymphoid tissue, such as tonsils, adenoids, Peyers patches in the small bowel and even the appendix. Taking advantage of the drainage system of the lymphatics, cancer cells enter the lymphatic vessels and then the lymph nodes. In general, the lymph nodes may serve as a gateway in the majority of cases in early cancer. Occasionally, the cancer cells may enter the blood vessels. This review article emphasizes the structural integrity of the lymphatic system through which cancer cells may spread. Using melanoma and breast cancer sentinel lymph node model systems, the spread of early cancer through the lymphatic system is progressive in a majority of cases. The lymphatic systems of the internal organs are much more complicated and difficult to study. Knowledge from melanoma and breast cancer spread to the sentinel lymph node may establish the basic principles of cancer metastasis. The goal of this review article is to emphasize the complexity of the lymphatic system. To date, the molecular mechanisms of cancer spread from the cancer microenvironment to the sentinel lymph node and distant sites are still poorly understood and their elucidation should take major priority in cancer metastasis research.

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