Necessity of a Functional Octadecanoic Pathway for Indole Alkaloid Synthesis by Catharanthus roseus Cell Suspensions Cultured in an Auxin-Starved Medium

Gene-to-metabolite networks for terpenoid indole alkaloid biosynthesis in Catharanthus roseus cells

“…Therefore, it seemed most promising to investigate the combined effects of these two hormones on TIA accumulation in C. roseus cells, applying growth and elicitation conditions similar to those described in ref. 11. TIA-targeted metabolite profiling showed pronounced accumulation of tabersonine and ajmalicine within 4 h and catharanthine within 12 h after MeJA elicitation (Fig.…”

“…The negative effect of auxins on ajmalicine levels has also been observed in ref. 11, but the levels of other monomeric TIAs were not assessed in that study.…”

“…Before initiating functional genomics-driven gene discovery for TIA metabolism in periwinkle cells, we needed to identify the conditions in which differential accumulation of the desired metabolites can be observed (10). In the case of C. roseus, the literature suggests that TIA accumulation is strongly influenced by the complex interaction of phytohormones such as auxins and jasmonates (11). Therefore, it seemed most promising to investigate the combined effects of these two hormones on TIA accumulation in C. roseus cells, applying growth and elicitation conditions similar to those described in ref.…”

Geraniol 10‐hydroxylase1, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis

“…The plant stress hormone (methyl)jasmonic acid ((Me)JA) induces the formation of TIAs in cell suspension cultures [24] and in seedlings [25]. It does so by coordinately switching on the expression of multiple genes in the TIA biosynthetic pathway [5,20,21] and precursor pathways [5].…”

Woody Stem Galls Interact With Foliage to Affect Community Associations

“…DIECA rapidly and efficiently causes the chemical reduction of hydroperoxyoctadecatrienoic acid, the product of the oxidation of linolenic acid by lipoxygenase, which prevents its cyclization and subsequent conversion to downstream products, including JA (Farmer et al 1994). DIECA has been successfully used as a JAinhibitor on a wide range of plant species (Piel et al 1997;Gantet et al 1998;Lee et al 1998;Menke et al 1999;Watanabe et al 2000; Garcia-Ponce and Rocha-Sosa 2000; Bulgakov et al 2004;Saltveit et al 2005;Hu and Zhong 2008;Lee et al 2008;Peebles et al 2009). Elucidation of how JA manipulation influences gall characteristics could improve the interpretation of studies that investigate gall ecology.…”