Necrosis-like death with plasma membrane damage against cervical cancer cells by photodynamic therapy

Abstract

In order to elucidate the antitumor effect of photodynamic therapy (PDT) using the photosensitizing agent hematoporphyrin derivative (Photogem) and a diode laser, we evaluated the cell death of uterine cancer cell lines (CaSki, HT3, HeLa, and SKOV-3) and mice transplanted with TC-1 lung cancer cells. Morphological changes, MTT assay, flow cytometry, cytotoxicity, and tumor growth-inhibition study were evaluated at various time intervals after PDT. The results showed that the survival rates of each cell line decreased with time and dose-response after performing PDT. Also, PDT-induced damage of cancer cells was almost entirely confined to necrosis of the tumor cells in the early time courses. The irradiation of CaSki cells in the presence of Photogem induced plasma membrane disruption and cell shrinkage, indicating the plasma membrane as the main target for Photogem. In the experiment in vivo, the time courses of Photogem with irradiation showed significantly longer survival and a significantly smaller tumor size compared to those in the untreated control groups, and resorption of the tumor after PDT treatment was observed. Collectively, our results indicated that Photogem possesses tumor-specific affinity, and necrosis-like death with plasma membrane damage was postulated to be the principal mechanism of the antitumor effect of PDT using Photogem.

A study on the mechanism of 5-aminolevulinic acid photodynamic therapy in vitro and in vivo in cervical cancer

“…In which the most significant effect of tumor size reduced the most, observed at topical treatment 7-14 days post-PDT. Our data are in agreement with the result of Ahn et al (26). It is helpful in deciding the optimal timing of the second PDT treatment.…”

TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells

“…Reports suggest that several factors could affect the difference in death mechanisms that are observed in TALEN-mediated gene editing such as TALEN spacer region, and selection of target region. Necrosis with plasma membrane damage was reported as the mode of cell death in cervical cancer cell lines after photodynamic therapy 32 . Our study also indicated necrotic cell death which could possibly activate pro-inflammatory cytokines leading to tumor-specific immunity.…”

Photodynamic therapy of head and neck basal cell carcinoma according to different clinicopathologic features

“…Necrosis like death with plasma membrane damage was postulated to be the principle mechanism of the anti-tumor effect of PDT using photoheme. We used Photoheme at a dose of 2-3.25 mg/kg 24 hours prior to activation of the drug with laser light (632 nm).The wave length used is quite close to the wavelength (630 nm) at which photoheme is activated the most [40,41].…”