Necrotizing Fasciitis Associated with Klebsiella pneumoniae Liver Abscess

Hu, Bor-Shen; Lau, Yeu-Jun; Shi, Zhi-Yuan; Lin, Yu‐Hui

doi:10.1086/313471

Cited by 53 publications

(35 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extra-hepatic complications spread by blood stream such as endophthalmitis, fasciitis and meningitis have been reported [1,[17][18][19]. The syndrome was subsequently reported in Asia and America [20,21].…”

Section: Discussionmentioning

confidence: 99%

Contribution of Outer Membrane Porin (Omp) K36 to Klebsiella pneumoniae induced Liver Abscess

Chang¹,

Tsai²,

Chen³

2016

Int J Clin Med Microbiol

View full text Add to dashboard Cite

Introduction: Pyogenic liver abscess is usually caused by multiple bacterial infections. This disease can be caused by single microorganism, Klebsiella pneumoniae that has been reported in Asia, North America and Europe. Many virulence factors of K. pneumoniae have identified including capsular polysaccharide, lipopolysaccharide, serum resistance, adhesins and siderophores. Previously, we demonstrated the virulent effect of outer membrane porin (OMP) K 36 by a lethality study. However, the role of OmpK36 in liver abscess pathogenesis is still unclear.Objective: In this study, a virulent K. pneumoniae strain NVT-1002 and OmpK36 deficient mutant (ΔOMPK) were used to examine the contribution of OmpK36 to liver abscess. Liver injuries and inflammatory cytokines expression were detected by tissue section and enzyme linked-immunosorbent assay respectively. The toxic effect of OmpK36 to human hepatoma cell (HepG2) was tested by bacterial infections and the treatment with OmpK36 recombinant protein. Results:The Results demonstrated that the NVT-1002 induced sever liver injuries while little or no damage was found in mice injected with OmpK36. Expression of serum and liver cytokines including TNF-α, IL-1β, IL-6 and IL-10 were elevated after injection of NVT-1002, meanwhile, only IL-1β was transiently elevated in OmpK36 group. Interestingly, OmpK36 recombinant protein showed no toxic effect to HepG2 cells, and the virulence of OmpK36 mutant to HepG2 was not altered compared to NVT-1002 group. Conclusion:In conclusion, OmpK36 contributes to the K. pneumoniae induced liver abscess and inflammatory responses. The virulent effect of OmpK36 is not mediated by the protein itself, but other uncertain mechanisms. OmpK36 may be a therapeutic target in K. pneumoniae infection. Contribution of Outer Keywords:OmpK36, Virulence, Liver abscess, Inflammatory Original ArticleOpen Access IntroductionKlebsiella pneumoniae have been documented as the common factor for cryptogenic liver abscess in Asia Pacific [1,2]. Similar findings in North America and Europe have also been reported [3][4][5]. Several virulent factors of K. pneumoniae were discovered and these factors are almost related to specific capsular polysaccharides (CPS) [2,[6][7][8]. Accumulating reports have indicated that the capsule is essential to the virulence of Klebsiella species [9][10][11]. Up to now, the capsular antigen have been classified into 77 serotypes , and serotype K1 and K2 capsular antigen were found to comprise virulence in mouse peritonitis model, whereas isolates of serotypes other than K1 or K2 were with little or no virulence [5,12].Outer membrane porins (OMP) are proteins that cross a cellular membrane and contribute to the diffusion of molecules. Many studies have showed OMPs play important roles in antimicrobial resistance [13,14]. Previously, we have demonstrated the virulent effects of OMPK 36 by an animal study [15]. In this report, the results of animal lethality study showed that the virulence of OmpK36 mutant was reduced about 100 fold c...

show abstract

Section: Discussionmentioning

confidence: 99%

Contribution of Outer Membrane Porin (Omp) K36 to Klebsiella pneumoniae induced Liver Abscess

Chang¹,

Tsai²,

Chen³

2016

Int J Clin Med Microbiol

View full text Add to dashboard Cite

show abstract

“…Therefore, it is always essential to look for other infectious foci in cases of soft tissue infections due to Klebsiella. 5,7,8 In a single hospital study conducted in Taiwan, Klebsiella was found to be responsible for 17% of monomicrobial NF, and held a higher mortality rate. Moreover, 80% of these patients were diabetic.…”

Section: Discussionmentioning

confidence: 99%

“…It is usually associated with predisposing conditions like diabetes mellitus or may occur as disseminated lesions from other infectious sites. 4 The case being reported is of a diabetic patient with chronic eczema who presented with acute NF of left leg following local application of steroids. Klebsiella being the causative organism is rather uncommom, especially in the absence of other infectious foci.…”

Section: Introductionmentioning

confidence: 99%

A case of necrotizing fasciitis due to Klebsiella following local application of steroids

Abdul

George

2016

Asian J Med Sci

View full text Add to dashboard Cite

Klebsiella organisms are known to cause a spectrum of clinical syndromes in human beings which include pneumonia, urinary tract infection, abdominal infection, surgical site infection, soft tissue infection and infection of intravascular devices. The incidence is higher among immunocompromised individuals and those with chronic debilitating diseases like diabetes, alcoholism etc. We present an elderly diabetic male who developed necrotizing fasciitis of the leg following local application of steroids. The causative organism turned out to be Klebsiella, which is an unusual pathogen.Moreover, necrotizing fasciitis due to Kleibsella in the absence of other infectious foci is a rare scenario.

show abstract

“…They were associated with other septic foci, commonly liver abscesses, urinary tract infections and endogenous endophthalmitis. Therefore, it is always essential to look for other foci of infection in case of Klebsiella associated soft tissue infections [14][15][16]. Due to increasing emergence of resistance, treatment of these infections have become challenging, often requiring a combination therapy [1].…”

Section: Discussionmentioning

confidence: 99%

Multiple Abscesses in Thigh due to Klebsiella in a Diabetic Patient with Rheumatoid Arthritis

Manappallil¹,

Rehaman²,

Subhash³

2016

JCR

View full text Add to dashboard Cite

Abstract:Klebsiella, a gram negative bacteria, can cause a variety of clinical syndromes in human beings. These include pneumonia, urinary tract infection, abdominal infection, surgical site infection, soft tissue infection and infections of intravascular devices. Middle-aged and elderly individuals with chronic debilitating diseases like diabetes, alcoholism, and immunocompromised persons are more susceptible to Klebsiella infections. This case report is regarding a diabetic patient, on treatment for rheumatoid arthritis, who developed cellulitis of leg with multiple thigh abscesses due to Klebsiella, which happens to be a rare presentation.

show abstract

Necrotizing Fasciitis Associated with Klebsiella pneumoniae Liver Abscess

Cited by 53 publications

References 3 publications

Contribution of Outer Membrane Porin (Omp) K36 to Klebsiella pneumoniae induced Liver Abscess

Contribution of Outer Membrane Porin (Omp) K36 to Klebsiella pneumoniae induced Liver Abscess

A case of necrotizing fasciitis due to Klebsiella following local application of steroids

Multiple Abscesses in Thigh due to Klebsiella in a Diabetic Patient with Rheumatoid Arthritis

Contact Info

Product

Resources

About