Necrotizing Keratitis Following Trabeculectomy With Mitomycin

Oram, Osman

doi:10.1001/archopht.1995.01100010021008

Cited by 17 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…wound healing [8,9,12,13], scleral blanching [6,19] and melting [20][21][22][23], and corneal per foration in cases of pterygium and late infec tion due to filtering bleb with avascular and thin walls [13,14], necrotizing keratitis [24] and persistent hypotonic maculopathy [25][26][27][28] because of overfiltration in the case of glaucoma.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Characteristics of Mitomycin C

Murayama¹,

Takahashi

Ikoma³

1996

Ophthalmic Res

View full text Add to dashboard Cite

In order to evaluate the cytotoxicity of mitomycin C (MMC), the effects of MMC on cell viability, cell cycle and cell surface structure were studied using Chang’s cultured human conjunctival cells. A 4- or 2-min exposure to MMC at concentrations ranging from 0.002 to 0.04% decreased the cell viability. The cell number was remarkably reduced by exposure to MMC over 0.004%. MMC at a concentration higher than 0.001% arrested the DNA synthetic phase of the cell cycle. MMC even at a high concentration (0.04%) did not cause any direct alteration to the cell surface.

show abstract

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and Characteristics of Mitomycin C

Murayama¹,

Takahashi

Ikoma³

1996

Ophthalmic Res

View full text Add to dashboard Cite

show abstract

“…The recurrence of GP observed with MMC alone was 57.1% with a postoperative follow-up period of 12-18 months [24]. MMC-related complications, e.g., ocular discomfort, eyelid erythema, corneal epithelial defects, corneal and scleral melting were reported [33][34][35]. No recurrence of GP was seen when AMT was used alone after resection but an exacerbation of VKC symptoms was seen in 69.2% of cases over a follow-up period of 14.2 +/− 4.2 months [26].…”

Section: Discussionmentioning

confidence: 99%

Long Term Outcomes of Surgical Excision of Giant Papillae with Mitomycin C and Amniotic Membrane Transplantation in the Treatment of Refractory Palpebral Vernal Keratoconjunctivitis

Patil

2021

Medicina

View full text Add to dashboard Cite

Background and Objectives: To report the long-term outcomes of patients with refractory Vernal Keratoconjunctivitis (VKC) who underwent surgical excision of giant papillae (GP) with mitomycin C (MMC) 0.02% and amniotic membrane transplantation (AMT). Materials and Methods: This is a retrospective interventional single-center case series including five eyes of four patients who had refractory, symptomatic VKC with GP, along with corneal shield ulcers and/or punctate epithelial erosions. They underwent surgical excision of GP with MMC 0.02% alone (1 eye) or with MMC 0.02% and AMT (4 eyes). Their long-term visual and surgical outcomes were studied. Results: All subjects were male with bilateral involvement and mean age of presentation 9.8 years. The surgery was uneventful in all cases. Amongst the four eyes which underwent MMC with AMT, only one eye demonstrated papillary regrowth requiring repeat surgery. Postoperative follow-up ranged from 59 to 77 months (median 66 months). Four patients had the best corrected visual acuity (BCVA) >/= 6/9.5. One patient had BCVA 6/15 at the final follow-up due to the presence of anterior corneal stromal scar and poor ocular surface. Conclusions: Surgical excision of GP in combination with MMC and AMT, in refractory VKC, is a good treatment option with better clinical outcomes over a longer follow-up.

show abstract

“…When MMC is fully applied, the wound cannot be repaired because of cell death and the recurrence of the disease may be impeded. However, application of excess MMC will cause cell death in a broad and deep range of the ocular tissue and side effects may occur such as hypotonic maculopathy [6,9,13,14] due to overfiltration, necrotizing keratitis [15] and late infection [16] due to thin filtering bleb and so on after glaucoma surgery. Scleromalacia after pterygium resection as previously reported [17] is also considered to result from the same mechanism as mentioned above.…”

Section: Discussionmentioning

confidence: 99%

Cell Growth Inhibition and DNA Incorporation of Mitomycin C in Cell Culture

Takahashi

Murayama²,

Oda³

et al. 1998

Ophthalmic Res

View full text Add to dashboard Cite

The present study was performed to clarify the effects of a 4-min exposure of mitomycin C (MMC) on cell growth, the cell cycle and MMC dose incorporated into DNA, using Chang’s cultured human conjunctival cells. A low dose of MMC ranging from 0.00025 to 0.004% showed dose-dependent cytotoxicity when cell growth was active. Fifty percent cell viability was found when cells were treated with 0.001% MMC. A flow cytometer showed that 0.001% MMC inhibited the DNA synthetic phase. After 0.04% MMC was exposed to 3 ×10⁶ cells and immediately rinsed, DNA was isolated to measure the dose of MMC detected from DNA. The total amount of DNA was 7 µg from which 3 µg of MMC was detected by high performance liquid chromatography. The above results revealed that the lowest concentration of MMC which caused 50% cell viability and cell cycle inhibition was 0.001% and that MMC was rapidly incorporated into DNA.

show abstract

Necrotizing Keratitis Following Trabeculectomy With Mitomycin

Cited by 17 publications

References 4 publications

Cytotoxicity and Characteristics of Mitomycin C

Cytotoxicity and Characteristics of Mitomycin C

Long Term Outcomes of Surgical Excision of Giant Papillae with Mitomycin C and Amniotic Membrane Transplantation in the Treatment of Refractory Palpebral Vernal Keratoconjunctivitis

Cell Growth Inhibition and DNA Incorporation of Mitomycin C in Cell Culture

Contact Info

Product

Resources

About