Negative regulation of primitive hematopoiesis by the FGF signaling pathway

Nakazawa, Fumie; Nagai, Hiroki; Shin, Masahiro; Sheng, Guojun

doi:10.1182/blood-2006-05-021386

Cited by 69 publications

(88 citation statements)

References 81 publications

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“…Cells in these clusters have the morphology of less mature blood cells ( Fig. 2A-C), similar to differentiating primitive blood cells before the initiation of circulation (Nakazawa et al, 2006). Larger clusters surrounding major arteries were observed to have both intravascular ( Fig.…”

Section: Definitive Erythrocytes Are Generated In Venous Vessels Adjamentioning

confidence: 79%

“…Primitive erythropoiesis in chickens is preceded by the expression of several hematopoietic transcriptional regulators, including gata1, scl, and lmo2 (Nakazawa et al, 2006). Genetic and molecular analyses in mice indicated that these regulators are critical for both primitive (Warren et al, 1994;Shivdasani et al, 1995;Porcher et al, 1996;Robb et al, 1996;Takahashi et al, 1997) and definitive erythropoiesis (Shivdasani et al, 1995;Porcher et al, 1996;Robb et al, 1996;Yamada et al, 1998;Shimizu et al, 2001).…”

Section: Definitive Erythropoiesis In the Yolk Sac Is Accompanied By mentioning

confidence: 99%

“…During early primitive erythropoiesis, lmo2 and scl mark hemangioblast cells prior to terminal differentiation (Nakazawa et al, 2006). After terminal differentiation of primitive erythrocytes, scl expression becomes restricted to blood cells, whereas lmo2 is expressed in both blood and endothelial cells (Nakazawa et al, 2006;Weng et al, 2007).…”

Section: Pre-existing Vascular Endothelium Is a Likely Source For Defmentioning

confidence: 99%

“…Second, the concentra- tion and duration of Proteinase K treatment were adjusted to increase probe penetration (twice the normal concentration and/or twice the normal treatment time, depending on the age of the yolk sac). The corresponding regions for gata1, scl, and lmo2 probes have been described previously (Nakazawa et al, 2006). After in situ hybridization, yolk sac tissues were photographed whole-mount with Olympus SZX12 microscope (Olympus, Tokyo, Japan) and Olympus DP70 camera, followed by paraffin embedded sectioning.…”

Section: Embryology In Situ Hybridization and Histologymentioning

confidence: 99%

“…It is unclear when and where hematopoietic cells generated from the dorsal aorta initiate terminal differentiation to supply definitive erythrocytes for the developing embryo. Primitive erythrocytes in chickens express mainly rho and epsilon beta globins and definitive erythrocytes mainly betaA beta globin (Nakazawa et al, 2006;Alev et al, 2008). Globin transcript and protein analyses (Simons, 1966;Wilt, 1967;Shimizu, 1972;Bruns and Ingram, 1973;Minie et al, 1992;Mason et al, 1995;Singal and vanWert, 2001) and erythrocyte morphological analysis (Fraser, 1961;Lucas and Jamroz, 1961;Minie et al, 1992) indicate that definitive erythrocytes in chickens appear in circulation at about E5.…”

Section: Introductionmentioning

confidence: 99%

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Definitive erythropoiesis in chicken yolk sac

Nagai¹,

Sheng²

2008

Developmental Dynamics

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The first wave of erythropoiesis in amniotic animals generates all primitive erythrocytes and takes place exclusively in yolk sac mesoderm. It is less clear, however, to what extend and for how long the yolk sac contributes to the second wave of erythropoiesis which gives rise to definitive erythrocytes for later embryonic and adult use. Here, we examine the initiation, duration, and site of definitive erythrocyte formation in chicken yolk sac. We show that the earliest definitive erythrocytes are generated in yolk sac venous vessels surrounding major arteries at embryonic day (E) 4 -4.5, and that mature definitive erythrocytes enter circulating at E4.5-E5. This takes place at a time when yolk sac vasculature remodels extensively to generate paired arterial/venous vessels. The yolk sac remains the predominant site for definitive erythropoiesis from E5 to E10, and continues to generate definitive erythrocytes at least until E15. Similar to primitive erythropoiesis, definitive erythropoiesis in the yolk sac is accompanied by the expression of transcriptional regulators gata1, scl, and lmo2. Furthermore, our data suggest that one main source of definitive erythropoietic cells is the pre-existing vascular endothelial cells. It remains unclear whether yolk sac derived hematopoietic progenitors that do not undergo erythropoiesis in the yolk sac may take up intraembryonic niches and contribute to erythropoietic stem cell population after hatching.

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Section: Definitive Erythrocytes Are Generated In Venous Vessels Adjamentioning

confidence: 79%

Section: Definitive Erythropoiesis In the Yolk Sac Is Accompanied By mentioning

confidence: 99%

Section: Pre-existing Vascular Endothelium Is a Likely Source For Defmentioning

confidence: 99%

Section: Embryology In Situ Hybridization and Histologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Definitive erythropoiesis in chicken yolk sac

Nagai¹,

Sheng²

2008

Developmental Dynamics

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fgf1 is required for normal differentiation of erythrocytes in zebrafish primitive hematopoiesis

Songhet

Adzic

Reibe

et al. 2007

Developmental Dynamics

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BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken

Alev¹,

McIntyre²,

Nagai³

et al. 2008

Developmental Dynamics

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Reflecting physiological changes in oxygen acquisition and regulatory changes in globin transcription, the makeup of globin chains in erythrocytes varies in development and disease. The relationship between the globin chain composition and erythropoietic lineages/niches is not well-understood. Using a combination of proteomic-, genomic-, and intron-based in situ hybridization analyses, we show that the transcripts and protein product of the major adult beta globin, betaA, are present as early as the major embryonic beta globins during chicken primitive erythropoiesis. A rapid rise in betaA percentage is seen from embryonic day (E) 5, reaching adult profile by E7. Our data suggest that betaA locus is active from the onset of primitive erythropoiesis and that beta globin switching during development may reflect a change in relative transcript abundance rather than a strict on/off switch in gene activation.

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Negative regulation of primitive hematopoiesis by the FGF signaling pathway

Cited by 69 publications

References 81 publications

Definitive erythropoiesis in chicken yolk sac

Definitive erythropoiesis in chicken yolk sac

fgf1 is required for normal differentiation of erythrocytes in zebrafish primitive hematopoiesis

BetaA, the major beta globin in definitive red blood cells, is present from the onset of primitive erythropoiesis in chicken

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