Negative visualization of DNA in agarose gel by eosin Y and its related mechanism

Cong, Weitao; You, Weijing; Chen, Mao; Jin, Ling; Zhu, Zhongxin; Choi, Jung-Kap; Jin, Litai; Li, Xiaokun

doi:10.1039/c2an00008c

Cited by 8 publications

(3 citation statements)

References 29 publications

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“…These defects can not only improve the solubility but also significantly enhance the reactivity and electronic property. In the same time, Eosin Y (EY), as a xanthene dye containing bromine atoms, is widely applied as the biological stain, laser dye, fluorescent probe, and sensitizer due to its high stability, versatility, and light absorptivity333435363738. Recently, EY was successfully applied for the fabrication of electrodes to monitor catechol and hydroquinone39, sodium dodecyl sulfate40, and hydroquinone and catechol41.…”

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confidence: 99%

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

Zhu

Zhang

Wang

et al. 2016

Sci Rep

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This study aimed at developing simple, sensitive and rapid electrochemical approach to quantitatively determine and assess the toxicity of 2,4-dichlorophenol (2,4-DCP), a priority pollutant and has potential risk to public health through a novel poly(eosin Y, EY)/hydroxylated multi-walled carbon nanotubes composite modified electrode (PEY/MWNTs-OH/GCE). The distinct feature of this easy-fabricated electrode was the synergistic coupling effect between EY and MWNTs-OH that enabled a high electrocatalytic activity to 2,4-DCP. Under optimum conditions, the oxidation peak current enhanced linearly with concentration increasing from 0.005 to 0.1 μM and 0.2 to 40.0 μM, and revealed the detection limit of 1.5 nM. Moreover, the PEY/MWNTs-OH/GCE exhibited excellent electrocatalytic activity toward intracellular electroactive species. Two sensitive electrochemical signals ascribed to guanine/xanthine and adenine/hypoxanthine in human hepatoma (HepG2) cells were detected simultaneously. The sensor was successfully applied to evaluate the toxicity of 2,4-DCP to HepG2 cells. The IC50 values based on the two electrochemical signals are 201.07 and 252.83 μM, respectively. This study established a sensitive platform for the comprehensive evaluation of 2,4-DCP and posed a great potential to simplify environmental toxicity monitoring.

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confidence: 99%

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

Zhu

Zhang

Wang

et al. 2016

Sci Rep

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“…The transformation experiments were essentially performed according to our previous study. 26 10 ml (5 ng ml À1 ) plasmid pGEM-T Easy (ampicillin resistance) was separated in 0.8% agarose gels and stained by VPBBO, EB, or NB as described above. Immediately aer staining, the DNA bands were excised and extracted according to the manufacturer's instructions for the EZNA Gel Extraction Kit.…”

Section: Transformation Experimentsmentioning

confidence: 99%

A shortcut organic dye-based staining method for the detection of DNA both in agarose and polyacrylamide gel electrophoresis

Cong

Chen

Zhu

et al. 2013

Analyst

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In this study, we describe a brief, sensitive and safe organic dye-based staining method for the visualization of DNA both in agarose and polyacrylamide gels by using Victoria Pure Blue BO (VPBBO). Down to 0.8-1.6 ng of λ DNA/HindIII markers in agarose gels and 0.4-0.8 ng of pUC18 DNA/Mspl markers in polyacrylamide gels can be successfully detected within 15 and 10 min by the new developed technique, respectively. Moreover, the mechanism of the VPBBO staining was investigated and further confirmed by electrospray ionization mass spectrometry (ESI-MS) and molecular docking. The results indicated that the interaction between VPBBO and DNA is mainly due to groove binding.

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“…Its structure is shown in Figure S1 of the Supporting Information. Because of its stability and complex aromatic structure, eosin Y is used in the fields of leather, printing, dyeing, printing ink, fluorescent pigment, photocatalyst, and so on. − Since it was used to act as a reversible stainer for detecting electrophoretically resolved proteins in 1986, eosin Y has rapidly been improved into a large-scale detection method for protein and DNA in polyacrylamide gel due to its high sensitivity and ease of use. − As a model for organic compounds, eosin Y has been rarely extended to interact with the cationic polymer. Up to now, there is no report on the interaction of Eosin Y, as a fluorescent probe, with PEI to form the eosin Y/PEI complex.…”

mentioning

confidence: 99%

A Turn-On Fluorescent Sensor for Sensitive and Selective Detection of Sodium Dodecyl Sulfate Based on the Eosin Y/Polyethyleneimine System

Wen

Luo

2013

Anal. Chem.

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A novel sensing system has been designed for the detection of sodium dodecyl sulfate (SDS) based on the recovered fluorescence signal of eosin Y and polyethyleneimine (PEI) complex. The eosin Y reacted with PEI to form a complex by virtue of hydrophobic interaction as well as the electrostatic interaction, which resulted in a strong fluorescence quenching of the eosin Y. Subsequently, with the addition of SDS to the eosin Y/PEI system, a strong surface interaction and electrostatic interactions between PEI and SDS resulted in the formation of the PEI/SDS complex and the dissociation of the eosin Y/PEI complex, which led to the significant fluorescence recovery. Herein, we have demonstrated that this facile methodology can offer a rapid, reliable, and selective detection of SDS with a detection limit as low as 0.02 μg mL(-1) and a linear range from 0.4 to 6 μg mL(-1). Furthermore, the method has been successfully applied to the detection of SDS in real samples with satisfied recovery and accuracy. Overall, these results demonstrate that this method has great promise for environmental applications.

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Negative visualization of DNA in agarose gel by eosin Y and its related mechanism

Cited by 8 publications

References 29 publications

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

Quantitative determination and toxicity evaluation of 2,4-dichlorophenol using poly(eosin Y)/hydroxylated multi-walled carbon nanotubes modified electrode

A shortcut organic dye-based staining method for the detection of DNA both in agarose and polyacrylamide gel electrophoresis

A Turn-On Fluorescent Sensor for Sensitive and Selective Detection of Sodium Dodecyl Sulfate Based on the Eosin Y/Polyethyleneimine System

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