Neglected Niches

Lee, Adrian V.

doi:10.1007/978-1-4615-9412-3_3

Cited by 77 publications

(6 citation statements)

References 106 publications

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“…If cecal microbial communities are stable, all physical and physiological niches should be filled. However, if cecal microbial communities are immature or in flux, the possibility remains for opportunistic colonization by pathogens (Lee, 1985; Bailey & Coe, 1999). Real‐time quantification of Ca.…”

Section: Discussionmentioning

confidence: 99%

Succession in the intestinal microbiota of preadolescent turkeys

Scupham

2007

FEMS Microbiology Ecology

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In the present study, automated ribosomal intergenic spacer analysis (ARISA), library sequence analysis, real-time PCR detection of Bacteroides uniformis and Campylobacter coli and dot-blot hybridizations of Clostridiaceae were used to identify trends in microbial colonization of the ceca of male turkeys. Two separate trials were performed with six and five birds, respectively. ARISA community profiles identified a period of community transition at week 12 of age in both trials. A significant increase of Ca. coli was also detected at week 12 in one trial, suggesting a possible correlation between microbiota destabilization and pathogen prevalence. Libraries of ribosomal small subunit 16S genes representing weeks 9, 11, 12 and 14 of both trials were sequenced. Whereas fingerprint and sequence analyses indicated significant differences in the species composition between the two trials, in general sequence library and dot-blot analyses indicated that Clostridia-like species decreased in prevalence over time. While B. uniformis prevalence in the two trials rose from 7% and 0% of the library clones at week 9 to 84% and 79% at week 11, real-time PCR did not support these results, with only approximately twofold and sixfold increases in internal transcribed spacer copy numbers observed.

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Section: Discussionmentioning

confidence: 99%

Succession in the intestinal microbiota of preadolescent turkeys

Scupham

2007

FEMS Microbiology Ecology

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“…may provide protection against proliferation of deleterious bacteria. This is the case of Deferribacteres found in mammals, particularly in rodents (Lee, 1985). In rats and mice, Deferribacteres relatives (Mucispirillum schaedleri (Robertson et al, 2005)) are located in the mucus layer covering and protecting the gastrointestinal epithelial cells (Lee, 1980).…”

Section: Rimicaris Spp Midgut Tube Houses a Tight And Specific Symbiosismentioning

confidence: 99%

Anatomy and Symbiosis of the Digestive System of the Vent Shrimp Rimicaris Exoculata and Rimicaris Chacei Revealed Through Imaging Approaches

et al. 2022

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The shrimp Rimicaris exoculata and Rimicaris chacei are visually dominant fauna co-occurring at deep-sea hydrothermal sites of the Mid-Atlantic Ridge (MAR). Their co-existence was related to contrasted life-history traits, including differences in their diet and reliance on chemoautotrophic symbionts at the adult stage. Both species of shrimp are colonized by diversified chemosynthetic symbiotic microbial communities in their cephalothoracic cavity. Symbiotic association with bacteria was also evidenced in their digestive system, and the major lineages were identified through sequencing (with Mycoplasmatales in the foregut and Deferribacteres in the midgut) but their precise distribution within each host species was not assessed. For the first time, we used Fluorescence in situ Hybridization (FISH) to visualize these lineages and describe their association with digestive structures of their host. The aim of the study was to identify possible differences between host species that could be related to their different life-history traits. For this purpose, we developed new specific FISH probes targeting Deferribacteres and Mycoplasmatales lineages identified in the digestive system of these shrimp. Our FISH results showed a partitioning of the bacterial lineages according to the digestive organ corroborating sequencing data, and highlighted their association with specific anatomical structures. Despite morphological differences between the foreguts of R. exoculata and R. chacei that could be related to the adult diet, our FISH results showed overall similar distribution of digestive symbionts for the two host species. However, a more comprehensive study is needed with specimens at different life or molt stages to reveal potential host specific patterns. Such comparisons are now possible thanks to our newly designed FISH probes. The tools used in our study are valuable for tracking symbiont lineages in the environment, allowing a better understanding of their relationship with their host along its life cycle, including their acquisition mechanisms.

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“…may provide a protection against proliferation of deleterious bacteria. This is the case of Deferribacteres found in mammals, particularly in rodents (Lee, 1985). In rats and mice, Deferribacteres relatives (new genus and species: Mucispirillum schaedleri (Robertson et al, 2005)) are located in the mucus layer covering and protecting the gastrointestinal epithelial cells (Lee, 1980).…”

Section: Rimicaris Spp Midgut Tube Houses a Tight And Specific Symbiosismentioning

confidence: 99%

Anatomy and Symbiosis of the digestive system of the vent shrimps Rimicaris exoculata and Rimicaris chacei revealed through imaging approaches

Guéganton

Rouxel

Durand

et al. 2022

Preprint

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The shrimps Rimicaris exoculata and Rimicaris chacei are visually dominant fauna co-occurring at deep-sea hydrothermal sites of the Mid-Atlantic Ridge (MAR). Their co-existence was related to contrasted life-history traits, among which differences in their diet and reliance on chemoautotrophic symbionts at adult stage. Both shrimps are colonized by diversified chemosynthetic symbiotic microbial communities in their cephalothoracic cavity. Symbiotic association with bacteria was also evidenced in their digestive system, and the major lineages were identified through sequencing (Mycoplasmatales lineages mainly in the foregut and Deferribacteres lineages mainly in the midgut) but their clear distribution within each host species was not assessed. For the first time, we used Fluorescence in situ Hybridization (FISH) to visualize these lineages. Then, we described their association with digestive structures of both Rimicaris species. The aim was to identify possible differences between host species that could be related to their different life-history traits. For this purpose, we first developed specific FISH probes targeting Deferribacteres and Mycoplasmatales lineages identified in the digestive system of these shrimps. After signal specificity validation for each the new probe, we showed a partitioning of the bacterial lineages according to the digestive organ. Despite morphological differences between the foregut of R. exoculata and R. chacei that could be related to the adult diet, our FISH results showed overall similar distribution of digestive symbionts for the two host species. However, a more comprehensive study is needed with specimens at different life or molt stages to bring potentially host specific patterns out. Such comparative approach using FISH is now warranted thanks to our newly designed probes. These will be valuable tools to track symbiont lineages in the environment, allowing a better understanding of their relationship with their host along its life cycle, including acquisition mechanisms.

show abstract

Neglected Niches

Cited by 77 publications

References 106 publications

Succession in the intestinal microbiota of preadolescent turkeys

Succession in the intestinal microbiota of preadolescent turkeys

Anatomy and Symbiosis of the Digestive System of the Vent Shrimp Rimicaris Exoculata and Rimicaris Chacei Revealed Through Imaging Approaches

Anatomy and Symbiosis of the digestive system of the vent shrimps Rimicaris exoculata and Rimicaris chacei revealed through imaging approaches

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