Nell-1 Gene Modified Mesenchymal Stem Cells on Biomimetic Porous Nano-Hydroxyapatite/Polyamide 66 Scaffolds Effectively Prevent Nonunion in Rats

Wang, Xiaoyu; Ju, Fang; Li, Ang; Geng, Shuo; Sun, Jiamei; Liu, Renhao; Yang, Cheng-lin; Bi, Zhenggang

doi:10.1166/jbt.2016.1456

Cited by 4 publications

(1 citation statement)

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“…Different methods were employed to modify HA/P66 implants to promote osseointegration. Nell-1 gene-modified mesenchymal stem cells 9 and vascular endothelial growth factor 6 were applied in combination with HA/P66. However, these methods were used only in the experimental stage and were difficult to apply clinically.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine-induced hydroxyapatite coating facilitates hydroxyapatite/polyamide 66 implant osteogenesis: an in vitro and in vivo evaluation

Xu¹,

Li²,

Wu³

et al. 2018

IJN

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BackgroundHydroxyapatite/polyamide 66 (HA/P66) has been clinically used for several years owing to its good biocompatibility and bioactivity. However, it has been found that the osseointegration process of the HA/P66 implant takes a large amount of time because of the small amount of HA on its surface.MethodsTo increase the amount of HA and aid faster osseointegration, we prepared a HA coating using a biomimetic process assisted by polydopamine (PDA) on the HA/P66 substrate. The surface properties of the substrate modified by PDA and HA were characterized, and the capacity of biomaterials for osteogenic induction was investigated both in vitro and in vivo.ResultsThe HA coating was successfully prepared on the HA/P66 substrate and verified by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The HA coating remained firmly attached to the underlying PDA-HA/P66 substrate even after strong ultrasound treatment for 1 h, and the calcium and phosphorus of the HA coating was continuously released in vitro in a slow manner. The formation of the HA coating on the PDA film greatly increased the hydrophilicity and surface roughness of HA/P66. In cell-based experiments, as compared with the HA/P66 substrate, the HA coating formation on the PDA film could facilitate the functions of C3H10T1/2 cells, including cell adhesion, proliferation, spreading, alkaline phosphatase activity, calcium nodule formation, and expression of osteogenic differentiation-related proteins. In addition, the HA/P66 scaffolds modified with PDA and HA coatings were implanted in rabbit femoral condyles. At 8 weeks after surgery, micro-computed tomography scanning (micro-CT) and hematoxylin–eosin (HE) staining revealed that more new bones were formed around the HA/P66 scaffold that was modified with a PDA-assisted HA coating.ConclusionThese results indicate that the preparation of a PDA-assisted HA coating by using a biomimetic process significantly improves the capacity of biomaterials for osteogenic induction.

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Section: Introductionmentioning

confidence: 99%

Polydopamine-induced hydroxyapatite coating facilitates hydroxyapatite/polyamide 66 implant osteogenesis: an in vitro and in vivo evaluation

Xu¹,

Li²,

Wu³

et al. 2018

IJN

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The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect

Liu

Huang

Liu

et al. 2022

Tissue Eng Regen Med

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Use of a nano-hydroxyapatite/polyamide composite in anterior cervical subtotal corpectomy and fusion

Zhang

Dong

et al. 2021

mat express

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This study aimed to explore the safety and efficacy of using nano-hydroxyapatite/polyamide (N-HA/PA) composite in anterior cervical vertebral body subtotal corpectomy and interbody fusion. Total 50 patients with cervical spondylotic myelopathy were enrolled to undergo anterior cervical spondylectomy. Bone graft pedicles were compounded with N-HA/PA and intervertebral body fusion was performed. Study outcomes included surgical efficacy and the degree of fusion. Patients in whom vertebral body fusion was performed with N-HA/PA composite pedicles had significantly improved symptoms. The postoperative Japanese Orthopaedic Association scores increased to 18.56±4.37 from 11.37±3.52, reflecting an improvement rate of 87.3%. The composite pedicle fusion rate was 96.4%. Therefore, N-HA/PA composite pedicle as a bone graft material in fusion surgery provides significant therapeutic efficacy. Moreover, the composite pedicle fusion rate is high, making it ideal for anterior cervical vertebral body subtotal corpectomy and fusion.

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Nell-1 Gene Modified Mesenchymal Stem Cells on Biomimetic Porous Nano-Hydroxyapatite/Polyamide 66 Scaffolds Effectively Prevent Nonunion in Rats

Cited by 4 publications

References 0 publications

Polydopamine-induced hydroxyapatite coating facilitates hydroxyapatite/polyamide 66 implant osteogenesis: an in vitro and in vivo evaluation

Polydopamine-induced hydroxyapatite coating facilitates hydroxyapatite/polyamide 66 implant osteogenesis: an in vitro and in vivo evaluation

The Combination of Platelet Rich Plasma Gel, Human Umbilical Mesenchymal Stem Cells and Nanohydroxyapatite/polyamide 66 Promotes Angiogenesis and Bone Regeneration in Large Bone Defect

Use of a nano-hydroxyapatite/polyamide composite in anterior cervical subtotal corpectomy and fusion

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