Nemabiome metabarcoding reveals differences between gastrointestinal nematode species infecting co-grazed sheep and goats

Costa, Lívio Martins; Chaudhry, Umer; Silva, Carolina R.; Sousa, Dauana Mesquita; Silva, Naylene C.; Júnior, José Antônio Alves Cutrim; Brito, Danilo Rodrigues Barros; Sargison, Neil

doi:10.1016/j.vetpar.2020.109339

Cited by 11 publications

(6 citation statements)

References 51 publications

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“…Hence, we sampled whole typical area in different elevation distributed with domestic animals in the QTP and detected 20 species of GINs from the faeces of seven species of livestock with 80.3% infection rate. Further, we also found that the parasites of T. circumcincta were the dominant species (28.5%, 121/424) in this region, which is common in sheep and goats in other areas (Costa‐Junior et al., 2021; Hrabok et al., 2006) and also detected in large animals such as cattle, yaks and horses in this study. Up to now, there was a report on T. circumcincta ‐infected cattle, which was a detection of antibodies against the parasite in cattle faeces and serum (no microscopic examination done) (Cooke et al., 2019).…”

Section: Discussionsupporting

confidence: 80%

Prevalence and molecular identification of gastrointestinal nematodes in Qinghai‐Tibetan Plateau of China

Ai,

Zhang,

Wang

et al. 2023

Veterinary Medicine & Sci

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BackgroundGastrointestinal nematodes (GINs) have seriously affected the production and earnings of animal husbandry in various countries, while some species of GINs infect humans. At present, little is known about the species and prevalence of GINs in Qinghai‐Tibetan Plateau (QTP).MethodsIn this study, 528 fresh faecal samples were collected from typical areas in different altitudes with seven species of livestock in Qinghai, Tibet, Gansu and Yunnan Provinces. ITS‐2 rRNA gene of nematodes was employed to detect by PCR and sequencing analysis. Phylogenetic analysis of related sequences was performed using MEGA 6.0 software.ResultsThe overall prevalence of GINs was 80.3% with 20 species of GINs detected, while Teladorsagia circumcincta was the dominant one, and four of which were zoonotic species such as Trichostrongylus colubriformis, Trichostrongylus axei, Trichostrongylus vitrinus and Oesophagostomum stephanostomum.ConclusionThe study provided panoptic information on the prevalence and species diversity of GINs in QTP area, which is useful and valuable for reference of measure formulation in livestock husbandry and public health concerns. The parasites of T. circumcincta, Cylicocyclus nassatus, Strongylus edentatus, Cylicostephanus longibursatus, Telephlebia brevicauda, Cyathostomum catinatum, Mecistocirrus digitatus, Cooperia punctata, Cylicodontophorus bicoronatus, Nematodirus oiratianus and Oesophagostomum asperum were firstly reported the presence in QTP area. The study also showed that horse could be infected by T. circumcincta, goat could be infected by C. nassatus, cattle could be infected by S. edentatus and C. bicoronatus,and O. stephanostomum could infect yak, cattle and Mongolian sheep in worldwide. Nevertheless, more investigations are needed, such as microscopic examination, to accurately determine the species in the region.

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Section: Discussionsupporting

confidence: 80%

Prevalence and molecular identification of gastrointestinal nematodes in Qinghai‐Tibetan Plateau of China

Ai,

Zhang,

Wang

et al. 2023

Veterinary Medicine & Sci

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“…The nemabiome refers to the community of nematodes that inhabit a single host animal or environmental niche (Francis & Slapeta 2022). Nemabiome sequencing provides a detailed picture of the species composition of the gastrointestinal parasite community in large sample sets and has vast potential for applications in diagnostics, surveillance, and research (Avramenko et al 2017;Costa-Junior et al 2021;Sargison et al 2022;De Seram et al 2022). However, such an approach is yet to be applied to determine species composition of trematode parasites, including liver flukes, amphistomes, and schistosomes, infecting our livestock.…”

Section: Nucleic Acid Detection-based Diagnostic Assaysmentioning

confidence: 99%

Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

Aftab,

Raina,

Maxton

et al. 2024

J. Helminthol.

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Fasciolosis, caused by Fasciola hepatica and F. gigantica, is an impediment to the livestock industry’s expansion and has a massively negative socio-economic impact due to its widespread prevalence in livestock. It is a waterborne zoonosis affecting human populations in the countries where rural economies are associated with livestock rearing. Conventional diagnosis of Fasciola infection is done by detecting parasite eggs in the faeces of infected animals or by immunological methods. Accurate and quick immunodiagnosis of Fasciola infection in animals and humans is based on the detection of antibodies and specific antigens expressed in the prepatent stage of the parasite. Both molecular and serodiagnostic tests developed thus far have enhanced the reliability of Fasciola diagnosis in both man and animals but are not widely available in resource-poor nations. A pen-side diagnostic test based on a lateral flow assay or a DNA test like loop-mediated isothermal amplification (LAMP) would be simple, fast, and cost-effective, enabling clinicians to treat animals in a targeted manner and avoid the development of drug resistance to the limited flukicides. This review focuses on the recent advances made in the diagnosis of this parasite infection in animals and humans.

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“…Immunity to intestinal worms also develops more rapidly when compared to abomasal worms [36,37]. It has also been shown that immune responses against GINs in small ruminants is different in sheep when compared to goats [38]. For example, a study that was conducted in sheep in Germany showed GIN infections in farms vary by farm, age, and gender [39].…”

Section: Immune Responses To Gin Infectionsmentioning

confidence: 99%

Mucosal Immunity of Major Gastrointestinal Nematode Infections in Small Ruminants Can Be Harnessed to Develop New Prevention Strategies

Palkumbura,

Mahakapuge,

Wijesundera

et al. 2024

IJMS

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Gastrointestinal parasitic nematode (GIN) infections are the cause of severe losses to farmers in countries where small ruminants such as sheep and goat are the mainstay of livestock holdings. There is a need to develop effective and easy-to-administer anti-parasite vaccines in areas where anthelmintic resistance is rapidly rising due to the inefficient use of drugs currently available. In this review, we describe the most prevalent and economically significant group of GIN infections that infect small ruminants and the immune responses that occur in the host during infection with an emphasis on mucosal immunity. Furthermore, we outline the different prevention strategies that exist with a focus on whole and purified native parasite antigens as vaccine candidates and their possible oral–nasal administration as a part of an integrated parasite control toolbox in areas where drug resistance is on the rise.

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Nemabiome metabarcoding reveals differences between gastrointestinal nematode species infecting co-grazed sheep and goats

Cited by 11 publications

References 51 publications

Prevalence and molecular identification of gastrointestinal nematodes in Qinghai‐Tibetan Plateau of China

Prevalence and molecular identification of gastrointestinal nematodes in Qinghai‐Tibetan Plateau of China

Advances in diagnostic approaches to Fasciola infection in animals and humans: An overviews

Mucosal Immunity of Major Gastrointestinal Nematode Infections in Small Ruminants Can Be Harnessed to Develop New Prevention Strategies

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