Nematicidal endophytic bacteria obtained from plants

Abstract

Two hundred and six bacterial isolates were obtained from leaf, flower and stem of three healthy plants, Euphorbia pulcherrima Willd, Pyrethrum cinerariifolium Trev. and Heracleum candicans Wall. The nematicidal activity experiment showed that a total of 92 isolates displayed activity against Caenorhabditis elegans (Maupas) Dougherty, and 70 isolates resulted active against Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle. Strain LCB-3 exhibited the strongest nematicidal activity against both two nematodes. According to the 16S rDNA, the strain LCB-3 was identified as Brevundimonas diminuta. Based on bioassay-guided fractionation, a nematicidal metabolite (R)-(-)-2-ethylhexan-1-ol was obtained from LCB-3. The median lethal concentrations (LC 50 ) of the compound were 542.0 mg l -1 against C. elegans and 168.1 mg l -1 against B. xylophilus 48 h after treatment.

Molecular and culture-dependent analyses revealed similarities in the endophytic bacterial community composition of leaves from three rice (Oryza sativa) varieties

“…Bacteria from this genus have been isolated from rice as endophytic nitrogen‐fixing bacteria (Prakamhang et al ., ) and detected in low proportion in a 16S rRNA gene clone library from rice roots (Sun et al ., ). Besides diazotrophy, Brevundimonas can be beneficial to the plant because of its suppressive effect against pathogenic fungi (De Boer et al ., ) or its nematicidal ability (Zheng et al ., ). Brevundimonas has also been retrieved as endophyte from diverse plant tissues like cotton roots (Hallmann et al ., ), banana shoots (Thomas et al ., ), carrots (Surette et al ., ), and tomato leaves (Enya et al ., ).…”

Diversity and bioactivity of bacterial endophyte community of Cupressaceae

“…Endophytism of some species of Brevundimonas and Microbacterium in other host plants is already shown (Burch and Sarathchandra ; Zheng et al. ; Alves et al. ).…”

“…Also, Microbacterium resistens is an environmental bacterium that is isolated from human clinical specimens (Giammanco et al 2006;Gneiding et al 2008). Endophytism of some species of Brevundimonas and Microbacterium in other host plants is already shown (Burch and Sarathchandra 2006;Zheng et al 2008;Alves et al 2014). However, our finding suggests a novel niche for such human pathogenic bacteria and implicates disease epidemiological considerations.…”

Isolation and characterisation of rhizosphere bacteria active against Meloidogyne incognita , Phytophthora nicotianae and the root knot-black shank complex in tobacco

“…Additionally, four species ( B. tequilensis , S. marcescens , S. albidoflavus and S. neopeptinius ) produced antifungal compounds, whereas the other four species ( B. drentensis , S. marcescens subsp. sakuensis , B. diminuta and S. alboflavus ) exhibited nematicidal activity. Apart from the aforementioned species, the antagonistic activities of the remaining species against the phytopathogens were first characterised here, especially for their functions as DAB.…”