Nematophagous fungi from decomposing cattle faeces in Argentina

Saumell, Carlos; Fernández, A.; Fusé, L. A.; Rodríguez, M. E.; Sagüés, María Federica; Iglesias, Lucía

doi:10.1016/j.riam.2014.09.003

Cited by 19 publications

(17 citation statements)

References 35 publications

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“…comm.). The second study took place at the Faculty of Veterinary Sciences, Tandil, Argentina (37°19′18″S, 59°04′49″W), and used a local isolate of the same fungus (Saumell et al , 2015).…”

Section: Methodsmentioning

confidence: 99%

Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi

et al. 2015

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The possible environmental effects of the massive use of Duddingtonia flagrans for controlling sheep nematodes were evaluated in two regions. Non-supplemented faeces and faeces from sheep supplemented with D. flagrans were deposited three times on pasture plots and samples were collected 7 and 14 days post-deposition. Samples were cultured in agar-water (2%) with Panagrellus spp. to recover D. flagrans and other nematophagous fungi, and soil nematodes were extracted using Baermann funnels and counted. No significant differences in the populations of soil nematodes and fungi colonizing sheep faeces (P > 0.05) were observed between supplemented and non-supplemented groups, except in one sample. The topsoil in contact with the faeces was sampled 1-4 months post-deposition, revealing that, with one exception, D. flagrans did not persist in soil beyond 2 months post-deposition. Duddingtonia flagrans does not affect faecal colonization by other fungi and soil nematodes and, once deployed on pasture, does not survive for long periods in the environment.

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Section: Methodsmentioning

confidence: 99%

Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi

et al. 2015

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“…In this way, the fungus contributes to the prophylactic control of nematodes, reducing the number of L3 in pasture and minimizing the risk of reinfection (Knox et al , 2002; Mota et al , 2003). However, in field conditions, the environment is influenced by several factors (rain, solar radiation, air temperature and relative humidity) that can affect the dynamics of nematophagous fungus (Hasanzadeh et al , 2012; Saumell et al , 2015). Thus, the objective of this work was to evaluate the predatory activity of the nematophagous fungus D. flagrans on infective larvae of gastrointestinal nematodes from dairy heifers in pasture areas and in vitro , after gastrointestinal transit from faeces, soil and pasture.…”

Section: Introductionmentioning

confidence: 99%

Predatory activity of nematophagus fungus Duddingtonia flagrans in infective larvae after gastrointestinal transit: biological control in pasture areas and in vitro

et al. 2021

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Biological control is a strategy to decrease parasitic populations, and the action takes place through natural antagonists in the environment. We studied the predatory activity of the fungus Duddingtonia flagrans in infective larvae (L3) of gastrointestinal nematodes after gastrointestinal transit. Ten heifers were divided into two groups: treated (animals received pellets containing fungus) and control (animals received pellets without fungus). Twelve hours after administration, faeces samples were collected for in vitro efficacy tests. The animals then remained for 7 h in the experimental pasture area. At the end of this period, 20 faecal pads (ten treated and ten control) were selected at random. Pasture, faecal pad and soil collections occurred with an interval of 7 days, totalling four assessments. In vitro activity demonstrated that fungi effectively preyed on L3, achieving a reduction percentage of 88%. In the faecal pad of the pasture area, there was a difference (P < 0.05) between collections 3 and 4 for both groups; in the treated group a reduction of 65% was obtained, while in the control group there was an increase of 217% in the number of L3. The recovery of L3 in the soil and in the pasture was similar in both groups. There was no influence (P = 0.87) of the passage time on the fungus predatory activity. Duddingtonia flagrans demonstrated the ability to survive gastrointestinal transit in the animals, reducing the number of L3 in the faeces, indicating that this biological control has great potential in the control of worm infections.

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“…Animal digestive tract parasitic nematodes are a class of parasites that cause serious damage to livestock husbandry, leading to huge economic losses to the world's livestock industry each year [1] . In recent decades, the use of chemical anthelmintic drugs, to a large extent, has relieved nematode caused economic losses in livestock husbandry.…”

Section: Introductionmentioning

confidence: 99%

Arthrobotrys oligospora Rekombinant Çitinaz AO-379’nın Enzim Özellikleri ve Nematod İndirgeyici Aktivitesi

Zhong¹,

Chen²,

Gong³

et al. 2019

Kafkas Univ Vet Fak Derg

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Chitinase is an important virulence factor produced by nematode trapping fungi in the process of infection, and plays an important role in the cleavage of nematodes and their eggshells. In this study, the cDNA sequence of Arthrobotrys oligospora chitinase AO-379 was amplified by RT-PCR and inserted into the vector pPIC9K to induce the expression of AO-379 in Pichia pastoris GS115. The recombinant AO-379 (reAO-379) was purified by nickel ion affinity chromatography, and enzymological properties and nematode-degrading activity of reAO-379 was analyzed. SDS-PAGE and Western blot analysis showed that the reAO-379 with molecular weight of about 44 kDa was successfully obtained. The reAO-379 showed strong chitinase activity at pH 5.5 and 30°C. Using reAO-379 to treat Strongylus equinus, Caenorhabditis elegans and Haemonchus contortus for 12, 24, and 36 h , the killing rates of reAO-379 in S. equinus were 42%, 89% and 100%; in C. elegans were 50%, 90% and 97%; in and H. contortus were 53%, 62% and 84%, respectively. Using reA-379 to treat Fasciola hepatica and Dicrocoelium chinensis eggs for 24, 48 and 72 h, the degradation rates of reAO-379 were 12%, 43% and 65% in F. hepatica eggs, and were 15%, 33% and 55% in D. chinensis eggs, respectively. Our study suggests that the reAO-379 is potentially valuable for development of biological control agent against digestive tract nematodes in livestocks.

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Nematophagous fungi from decomposing cattle faeces in Argentina

Cited by 19 publications

References 35 publications

Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi

Lack of negative effects of the biological control agent Duddingtonia flagrans on soil nematodes and other nematophagous fungi

Predatory activity of nematophagus fungus Duddingtonia flagrans in infective larvae after gastrointestinal transit: biological control in pasture areas and in vitro

Arthrobotrys oligospora Rekombinant Çitinaz AO-379’nın Enzim Özellikleri ve Nematod İndirgeyici Aktivitesi

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