Neonatal Septicemia: Isolation, Identification and Antibiotic Sensitivity Pattern of Bacteria in a Tertiary Hospital in Bangladesh

Abstract

Abstract:A cross sectional descriptive study was done to find out the causative organisms and their antibiotic sensitivities in the Department of Microbiology in collaboration with the Department of Paediatrics, Rajshahi Medical College Hospital (RMCH), Rajshahi during the period of July 2014 to June 2015. A total of 116 blood samples were taken aseptically from patients who were suspected of neonatal septicemia. Blood was then inoculated in Brain heart infusion broth. Bacterial isolation, identification and antimicrobial susceptibility testing were done by standard microbiological methods. Among 116 cases, 33(28.4%) were found to be culture positive. The most commonly isolated causative agents of neonatal septicemia were Staphylococcus aureus 17(51.5%) followed by Escherichia coli 10(30.3%), Streptococcus pneumoniae 03(9.09%), Klebsiella pneumoniae 02(6.06%) and Pseudomonas aeruginosa 01(3.03%). In general, all the Gram positive and Gram negative isolates were highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin. Gram positive bacteria were found to be particularly sensitive to vancomycin. They were moderately sensitive to ceftazidime followed in amoxicillin but were totally resistant to ampicillin. This study revealed that Staphylococcus aureus and E.coli are predominant causative organisms in neonatal septicemia and these are highly sensitive to meropenem, amikacin, gentamicin and ciprofloxacin.

Admission Outcome and Antimicrobial Resistance Pattern of Bacterial Isolates among Neonates with Suspected Sepsis in Neonatal Intensive Care Unit at Dessie Comprehensive Specialized Hospital, Dessie, Northeastern Ethiopia

“…In the present study, the overall blood culture positive rate (27.2%) was in agreement with similar studies conducted in Bangladesh (28.4%) [25], Indonesia (24.6%) [26] and Assela, Ethiopia (29.4%) [20]. In contrast, it was lower than studies done in Gondar, Ethiopia (46.6%) [8], Egypt (40.7%) [27] and India (41.7%) [28].…”

Gram-negative neonatal sepsis in low- and lower-middle-income countries and WHO empirical antibiotic recommendations: A systematic review and meta-analysis

“…There was no evidence of asymmetry by Egger’s meta-regression test ( S1 Data ). There were 34 studies from the Africa region—Angola (1) [ 18 ], Congo (1) [ 19 ], Egypt (5) [ 20 – 24 ], Ethiopia (4) [ 25 – 28 ], Ghana (1) [ 29 ], Guinea (1) [ 30 ], Madagascar (1) [ 31 ], Malawi (1) [ 32 ], Nigeria (12) [ 28 , 33 – 43 ], Rwanda (1) [ 28 ], Sudan (1) [ 15 ], Tanzania (3) [ 44 – 46 ], Uganda (1) [ 47 ], and Zambia (1) [ 48 ]—and 59 studies from the Asia region—Bangladesh (4) [ 28 , 49 – 51 ], India (37) [ 5 , 28 , 52 – 86 ], Indonesia (2) [ 87 , 88 ], Nepal (9) [ 89 – 97 ], and Pakistan (7) [ 28 , 98 – 103 ]—as depicted in Fig 2 . One publication included data from 6 countries [ 28 ].…”

Treatment outcomes, antibiotic use and its resistance pattern among neonatal sepsis patients attending Bahawal Victoria Hospital, Pakistan

“…All neonates admitted in the Pediatric Ward 1 and Pediatric Ward 2 from January 1, 2019 to June 30, 2019, and diagnosed with EOS and LOS were included in the study. Neonates with extremely low birth weight (i.e., <1000 g) and those suffering from pneumonia, meningitis and congenital abnormalities were excluded from the study [ 13 , 25 , 26 ]. Similarly, those with incomplete medical records were also excluded from the study.…”