Neosporal Abortion in Oklahoma Cattle with Emphasis on the Distribution of Brain Lesions in Aborted Fetuses

Helman, R. G.; Stair, E L; Lehenbauer, Terry W.; Rodgers, Shuchi K.; Saliki, Jeremiah T.

doi:10.1177/104063879801000314

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…These results, compared with those obtained in the present study, reinforce the idea that the nervous tissue is the material of choice for diagnosing neosporosis (HELMAN et al, 1998;CORBELLINI et al, 2002;DUBEY, 2003;CABRAL et al, 2009).…”

Section: Discussionsupporting

confidence: 90%

“…The histopathological findings of non-suppurative encephalitis associated with tissue necrosis and gliosis, and the myositis and myocarditis found in the bovine fetuses of this study, are in accordance with the lesions described for neosporosis (LINDSAY et al, 1999;HELMAN et al, 1998;CORBELLINI et al, 2002;DUBEY, 2003;ORTEGA-MORA et al, 2006), as were the hepatic lesions of periportal mononuclear infiltrate and foci of hepatocellular necrosis (BARR et al, 1990).…”

Section: Discussionsupporting

confidence: 89%

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Neospora caninum in bovine fetuses of Minas Gerais, Brazil: genetic characteristics of rDNA

Andrade

Varaschin

Guimarães

et al. 2011

Rev. Bras. Parasitol. Vet.

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Neosporosis is an important cause of abortion in cattle and information on their genetics and host parasite relationships are desirable. Neospora caninum samples obtained from 24 bovine fetuses from Minas Gerais, were genetically analyzed in part of the rDNA region, coding for rRNAs. Previously, brain, heart, liver, skeletal muscle, lung, kidney, spleen, thymus, lymph nodes, ovary or testis, uterus and skin of the ear were analyzed by conventional histopathology and immunohistochemistry. Of these, eight had lesions compatible in the brain and heart and two also in skeletal muscle and liver. Three brains showed antigen identification in immunohistochemistry. Brain and heart tissues were subjected to DNA extraction for PCR, whose product of 588 bp of ITS-1 region was sequenced in three samples. We obtained 96% similarity with dozens of sequences N. caninum deposited in GenBank. The phylogenetic tree showed great conservation among isolates of N. caninum in this study and those deposited in GenBank, while welldefined and specific branches were generated against other Apicomplexa. PCR for this region is useful as a diagnosis, with good analytical specificity, but the ITS-1 region is not suitable for genetic differentiation intra species because the sequences obtained were identical to the others analyzed.Keywords: Bovine, diagnosis, phylogeny, Neospora caninum, PCR. ResumoA neosporose é uma importante causa de abortos em bovinos, e informações sobre sua genética e relação parasito-hospedeiro são desejáveis. Amostras de Neospora caninum, obtidas de 24 conceptos bovinos oriundos de Minas Gerais, foram analisadas geneticamente em parte da região rDNA, codificadora de rRNAs. Previamente, cérebro, coração, fígado, músculo esquelético, pulmão, rim, baço, timo, linfonodos, ovário ou testículo, útero e pele da orelha foram analisados por histopatologia convencional e imuno-histoquímica. Dessas, oito apresentaram lesões compatíveis no encéfalo e coração e dois também no músculo esquelético e fígado. Três cérebros apresentaram identificação antigênica na imuno-histoquímica. Tecidos cerebral e cardíaco foram submetidos a extração de DNA para PCR, cujo produto de 588 pb da região ITS-1 foi sequenciado em três amostras. Obteve-se similaridade de 96% com dezenas de sequências de N. caninum depositadas no GenBank. A árvore filogenética mostrou grande conservação entre isolados de N. caninum deste estudo e aqueles depositadas no GenBank, enquanto ramos bem definidos e específicos foram gerados em relação a outros apicomplexa. A PCR para esta região é útil como diagnóstico, com boa especificidade analítica, mas a região ITS-1 não é apropriada para a diferenciação genética intraespécie, pois as sequências obtidas foram idênticas às demais analisadas.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Neospora caninum in bovine fetuses of Minas Gerais, Brazil: genetic characteristics of rDNA

Andrade

Varaschin

Guimarães

et al. 2011

Rev. Bras. Parasitol. Vet.

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“…Brain lesions were also frequently found in the cerebrum, particularly the gray matter in aborted Neospora-infected fetuses (18). The cerebrum showed a significantly higher frequency of lesions than the medulla oblongata and cerebellum (18). Our findings are in accordance with previously published results.…”

supporting

confidence: 83%

“…Therefore, N. caninum may exhibit similar brain topology. Brain lesions were also frequently found in the cerebrum, particularly the gray matter in aborted Neospora-infected fetuses (18). The cerebrum showed a significantly higher frequency of lesions than the medulla oblongata and cerebellum (18).…”

mentioning

confidence: 95%

Tissue Distribution of Neospora caninum in Experimentally Infected Cattle

Nishimura

Kohara

Hiasa

et al. 2013

Clin Vaccine Immunol

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“…Abortamentos em bovinos causados por protozoários apicomplexas são provisoriamente diagnosticados através das lesões microscópicos características no feto como encefalite necrosante multifocal não-supurativa; miocardite não-supurativa e, menos freqüentemente, inflamação não-supurativa focal em outros órgãos, tais como músculo esquelético, fígado, pulmão e placenta (THILSTED; BARR et al, 1990BARR et al, , 1991aDUBEY et al, 1990c;THORNTON et al, 1991;ANDERSON et al, 1991ANDERSON et al, , 1995NIETFELD et al, 1992;OGINO et al, 1992;LINDSAY, 1993;LINDSAY et al, 1993, YAEGER et al, 1994BOULTON et al, 1995;MCALLISTER et al, 1996a;WOUDA et al, 1997b;HATTEL et al, 1998;HELMAN et al, 1998;MOEN et al, 1998;MORALES et al, 2001;CORBELLINI et al, 2002;KIM et al, 2002;MOORE et al, 2002;BOGER;HATTEL, 2003;MALEY et al, 2003;PEREIRA-BUENO et al, 2003;COLLANTES-FERNÁNDEZ et al, 2006b;GIBNEY et al, 2007;PESCADOR et al, 2007;REITT et al, 2007;REGIDOR-CERRILLO et al, 2008 , 1989a;ANDERSON et al, 1991;BARR et al, 1991a;NIETFELD et al, 1992;DUBEY et al, 1992b;LINDSAY, 1993;YAEGER et al, 1994;WOUDA et al, 1997b;HATTEL et al, 1998;…”

Section: Diagnóstico Histopatológicounclassified

Aspectos epidemiológicos da infecção por Neospora caninum em bovinos leiteiros da região do Vale do Paraíba Paulista

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Neosporal Abortion in Oklahoma Cattle with Emphasis on the Distribution of Brain Lesions in Aborted Fetuses

Cited by 12 publications

References 12 publications

Neospora caninum in bovine fetuses of Minas Gerais, Brazil: genetic characteristics of rDNA

Neospora caninum in bovine fetuses of Minas Gerais, Brazil: genetic characteristics of rDNA

Tissue Distribution of Neospora caninum in Experimentally Infected Cattle

Aspectos epidemiológicos da infecção por Neospora caninum em bovinos leiteiros da região do Vale do Paraíba Paulista

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