Nerol triggers mitochondrial dysfunction and disruption via elevation of Ca2+ and ROS in Candida albicans

Tian, Jun; Lu, Zhaoqun; Wang, Yanzhen; Zhang, Man; Wang, Xueyan; Tang, Xudong; Peng, Xue; Zeng, Hong

doi:10.1016/j.biocel.2017.02.006

Cited by 49 publications

(34 citation statements)

References 49 publications

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“…However, in TEA‐pretreated cells, the percentages of cells in certain cell cycle phases were similar to those in the untreated cells. In yeast, metacaspases, which are caspase‐like cysteine proteases, are related to mitochondrial dysfunction . In this study, cells with activated intracellular metacaspases exhibited more fluorescence than the untreated cells.…”

Section: Resultsmentioning

confidence: 57%

“…This suggested that TEA pretreatment reduced PS exposure, whereas DIDS pretreatment impaired silymarin‐induced PS externalization. DNA fragmentation is observed in the late stage of apoptosis . Silymarin‐treated cells exhibited increased TUNEL fluorescence intensity, compared to the untreated cells (Fig.…”

Section: Resultsmentioning

confidence: 89%

“…Silymarin‐induced loss of MMP resulted in PS externalization. Most membrane PS exists in the inner leaflet of the cytoplasmic membrane . When apoptosis occurs, PS translocated to the outer layer of the membrane while the membrane remains intact.…”

Section: Discussionmentioning

confidence: 99%

“…DNA fragmentation can be analyzed using the terminal deoxynucleotidyl transferase dUTP nick‐end labeling (TUNEL) assay, which is a common method to detect apoptotic DNA cleavage in individual nuclei by labeling fluorescent dUTP at the 3'‐OH ends of DNA . Cells were pretreated with 5 mM TEA and 0.5 mM DIDS for 10 min before the test compounds were added to the culture.…”

Section: Methodsmentioning

confidence: 99%

“…Caspases can be detected by FITC‐VAD‐FMK staining . VAD‐FMK, which is a fluorescein isothiocyanate (FITC)‐conjugated pancaspase inhibitor, was used to determine caspase activity.…”

Section: Methodsmentioning

confidence: 99%

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Potential role of potassium and chloride channels in regulation of silymarin‐induced apoptosis in Candida albicans

Lee

2018

IUBMB Life

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Silymarin, which is derived from the seeds of Silybum marianum, has been widely used to prevent and treat liver diseases. In our previous study, we reported that at concentrations above the minimal inhibitory concentration (MIC), silymarin exhibited antifungal activity against Candida albicans by targeting its plasma membrane. However, the antifungal mechanism at concentration below the MIC remains unknown. Therefore, we aimed to determine the underlying mechanism of antifungal effects of silymarin at concentration below the MIC. To evaluate the inhibitory effects on the ion channels, C. albicans cells were separately pretreated with potassium and chloride channel blockers. The antifungal activity of silymarin at sub-MIC was affected by the ion channel blockers. Potassium channel blockade inhibited the antifungal effects, whereas chloride channel blockade slightly enhanced these effects. Subsequently, we found that silymarin induced disturbances in calcium homeostasis via the cytosolic and mitochondrial accumulation of calcium. Furthermore, apoptotic responses, such as phosphatidylserine exposure, loss of mitochondrial membrane potential (MMP), DNA damage, and caspase activation were induced in response to silymarin treatment. The increases in intracellular calcium level and pro-apoptotic changes were prevented when potassium ion channels were blocked. In contrast, these changes were enhanced upon chloride channels blockade; however, this did not affect the intracellular calcium levels and MMP loss. Thus, we showed that silymarin treatment at concentration below the MIC induced apoptosis in C. albicans; additionally, ion channels contributed these effects. © 2018 IUBMB Life, 70(3):197-206, 2018.

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Section: Resultsmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Caspases can be detected by FITC‐VAD‐FMK staining . VAD‐FMK, which is a fluorescein isothiocyanate (FITC)‐conjugated pancaspase inhibitor, was used to determine caspase activity.…”

Section: Methodsmentioning

confidence: 99%

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Potential role of potassium and chloride channels in regulation of silymarin‐induced apoptosis in Candida albicans

Lee

2018

IUBMB Life

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Natural Monoterpenes: Much More than Only a Scent

Wojtunik-Kulesza

Kasprzak

Oniszczuk

et al. 2019

Chemistry & Biodiversity

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Terpenes are a widespread group of secondary metabolites that can be found in various family plants such as the Lamiaceae. In view of their numerous valuable biological activities, the industrial production of concrete terpenes and essential oils rich in the substances is intensively studied. Monoterpenes constitute a significant part of the whole group of the aforementioned secondary metabolites. This is due to their numerous biological activities and their ability to permeate the skin. Despite the fact that these substances have gain popularity, they are not comprehensively characterized. The presented review is based on studies of the biological activities of the most important monoterpenes and the essential oils rich in these compounds. The authors focused attention on antioxidant activity, inhibition towards acetyl-and butyrylcholinesterase, and α-amylase and α-glucosidase, antifungal, hepatoprotective, sedative properties, and their skin permeation enhancement.

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Synergistic Antifungal Activity of Isoquercitrin: Apoptosis and Membrane Permeabilization Related to Reactive Oxygen Species in Candida albicans

2018

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Isoquercitrin (ISO), one of phytochemical isolated from aerial parts of Aster yomena, has been reported to have antifungal activity. However, the synergistic effect and the mechanism of ISO in combination with conventional antifungal agents are poorly understood. Therefore, synergistic antifungal effect between ISO and conventional antifungal agents was investigated. ISO at non‐antifungal concentration interacts synergistically with amphotericin B (AMB) and fluconazole (FLC), but the combination with flucytosine (5‐FC) showed no interaction. ISO disrupted an antioxidant system by inhibiting the activity of superoxide dismutase. This redox imbalance was shown to induce intracellular reactive oxygen species (ROS) accumulation and oxidative stress. ISO combined with FLC caused metacaspase activation and DNA condensation, markers of apoptosis, higher than the combination with ISO/AMB. In contrast, ISO with AMB synergistically stimulated membrane permeabilization compared to ISO/FLC. Scavenging ROS consequently reduced the synergy‐induced apoptosis and membrane permeabilization, indicating combinations induced ROS were associated with the synergy effect of ISO. In conclusion, AMB and FLC enhanced the antifungal potency of ISO through oxidative stress when used in synergy. © 2018 IUBMB Life, 71(1):283–292, 2019

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Nerol triggers mitochondrial dysfunction and disruption via elevation of Ca2+ and ROS in Candida albicans

Cited by 49 publications

References 49 publications

Potential role of potassium and chloride channels in regulation of silymarin‐induced apoptosis in Candida albicans

Potential role of potassium and chloride channels in regulation of silymarin‐induced apoptosis in Candida albicans

Natural Monoterpenes: Much More than Only a Scent

Synergistic Antifungal Activity of Isoquercitrin: Apoptosis and Membrane Permeabilization Related to Reactive Oxygen Species in Candida albicans

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