Nerve growth factor levels in cultured human skin cells: effect of gestation and viral transformation

Anand, Praveen; Foley, Peter J.; Navsaria, Harshad; Sinicropi, Dominick; Williams-Chestnut, R.E.; Leigh, Irene M.

doi:10.1016/0304-3940(94)11195-o

Cited by 26 publications

(11 citation statements)

References 22 publications

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“…The antibody to recombinant human βNGF (rhNGF) was obtained from Genentech Inc., USA, and has been described previously ( Anand et al, 1995 ) . Antibodies to prepro‐NGF −60 to −91 and −144 to −163 were obtained from Pro‐Hormone Science (Torrance, CA, USA), and have been extensively characterized ( Reinshagen et al, 1997 ) .…”

Section: Methodsmentioning

confidence: 99%

Molecular forms of NGF in human and rat neuropathic tissues: decreased NGF precursor‐like immunoreactivity in human diabetic skin

Yiangou

Facer

Sinicropi

et al. 2002

J Peripheral Nervous Sys

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Precursors of NGF have been shown to be the predominant forms of this neurotrophin in human brain and peripheral tissues, and proNGF has been shown recently to preferentially bind p75NTR with high affinity. In our studies of human and rat skin and nerve extracts, a 53 kDa band was detected by Western blot using antibodies against rhNGF or prepro-NGF (-91 to -60), that could correspond to a previously described modified prepro-NGF-like molecule. The relative optical intensity of the 53-kDa bands was markedly reduced in skin extracts from patients with subclinical diabetic neuropathy (diabetic: 1.5, 1.0-8.0, n = 6; controls: 52.0, 23.0-85.0, n = 6, p = 0.0022) but was increased in extracts of inflamed colon from patients with Crohn's disease (inflamed: median 12.0, range 0.1-12.0, n = 11: controls: 0.1, 0.1-3.0, n = 8, p = 0.0055). Antibodies to both rhNGF and prepro-NGF immunostained basal keratinocytes in tissue sections of normal human and rat skin showed accumulation of immunoreactivity in nerve fibers distal to sciatic nerve ligation in rats. Prepro-NGF antibody immunostained rat large/medium sensory neurons, whereas only small sensory neurons were stained with antibodies to mature rhNGF, suggesting that prepro-NGF may be preferentially taken up and transported by p75NTR. The different molecular forms derived from prepro-NGF are likely to be of importance in sensory mechanisms, and deserve further investigation.

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Section: Methodsmentioning

confidence: 99%

Molecular forms of NGF in human and rat neuropathic tissues: decreased NGF precursor‐like immunoreactivity in human diabetic skin

Yiangou

Facer

Sinicropi

et al. 2002

J Peripheral Nervous Sys

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“…NGF is synthesized by basal keratinocytes in the epidermis[19], although some NGF mRNA may derive from Schwann cells[20] in the dermis. Due to the low abundance of NGF mRNA in skin biopsies as well as their small size, quantitative competitive reverse transcriptase polymerase chain reaction (cRT‐PCR) was employed as the only method available with adequate sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Increased nerve growth factor mRNA in lateral calf skin biopsies from diabetic patients

Diemel

Cai

Warner³

et al. 1999

Diabetic Medicine

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“…Decreased keratinocyte turnover is suggested as a cause of the decreased NGF, because NGF expression is greatest in dividing keratinocytes and statum corneum cell turnover is delayed in human diabetic skin. 19 Patients with diabetic neuropathy have lower serum NGF than controls, and the decreases in NGF are proportional to decreases in the patients' nerves' motor conduction velocities. 20 It is noted that diabetic neuropathy mainly affects the targets of NGF, namely, sensory and sympathetic neurons.…”

Section: Neuropeptides Affected By Denervationmentioning

confidence: 98%

Wound Healing in Denervated Tissue

Barker¹,

Rosson²,

Dellon³

2006

Annals of Plastic Surgery

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Sacral and trochanteric pressure sores in patients with plegias, and foot ulceration in patients with diabetic neuropathy, are similar because these wounds occur in tissues that do not have normal innervation. While it is recognized that insensitive tissue increases the likelihood of ulceration and recurrence of ulceration, this review attempts to answer the question, Is wound healing impaired in denervated tissue? A review of the scientific literature of the past 35 years demonstrates that all phases of wound healing are impaired in denervated tissue, and these mechanisms are different from those related to one of the underlying diseases, diabetes. Understanding the value of innervation, a goal of wound healing should be to seek strategies that provide reinnervation to these at-risk tissues.

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Nerve growth factor levels in cultured human skin cells: effect of gestation and viral transformation

Cited by 26 publications

References 22 publications

Molecular forms of NGF in human and rat neuropathic tissues: decreased NGF precursor‐like immunoreactivity in human diabetic skin

Molecular forms of NGF in human and rat neuropathic tissues: decreased NGF precursor‐like immunoreactivity in human diabetic skin

Increased nerve growth factor mRNA in lateral calf skin biopsies from diabetic patients

Wound Healing in Denervated Tissue

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