Nerve Growth Factor Stimulates Cellular Proliferation of Human Epithelial Ovarian Cancer

Urzúa, Ulises; Tapia, Verónica; Geraldo, M. P.; Selman, Alberto; Vega, Margarita; Romero, Carmen

doi:10.1055/s-0032-1304617

Cited by 21 publications

(37 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Such results evidenced an important increase of TRKA gene among EOC as compared to HOSE cells and also an increase in MAPK1, PI3K, and AKT2, without changes in FOXL2 gene [4] (Figure 1).…”

Section: Ngf and Trka In Eocmentioning

confidence: 77%

“…Ovarian cancer is the most lethal gynecological cancer; it remains with a low survival rate [2], and therefore, understanding the mechanisms governing angiogenesis is important in order to develop new strategies for its treatment. Neurotrophins have been shown to be involved in both normal ovarian and ovarian cancer angiogenesis [3,4]; thus, targeting their signaling signifies a novel therapeutic opportunity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of nerve growth factor and its TRKA receptor in normal ovarian and epithelial ovarian cancer angiogenesis

Vera¹,

Tapia²,

Vega³

et al. 2014

Journal of Ovarian Research

View full text Add to dashboard Cite

In normal ovarian function a controlled angiogenesis is essential. Several growth factors are involved in this process, such as the vascular endothelial growth factor (VEGF) and nerve growth factor (NGF). The angiogenesis process in the normal ovary is a tightly controlled process that occurs in each ovarian cycle. Also, angiogenesis is critical for ovarian cancer development and it is responsible for tumor spread, metastasis and its peritoneal dissemination. Ovarian cancer is the fifth leading cause of cancer death in women and it is distinguished as the most lethal gynecologic cancer. In recent years angiogenesis has been given considerable attention in order to identify targets for developing effective anti-tumor therapies. Several molecules have been reported to promote angiogenesis, such as platelet-derived growth factor (PDGF) and its receptors, the angiopoietin/Tie ligand/receptor system and fibroblast growth factor (FGF). Primarily, VEGF has been identified to play key roles in driving angiogenesis. The above-mentioned molecules are candidate drug targets. Used in combination with other treatments, anti-angiogenic therapies have managed to reduce disease progression. The present review is focused in NGF and its high affinity receptor tyrosine kinase A (TRKA). The expression of VEGF, proliferation and the angiogenesis process in ovarian cancer is importantly induced by NGF, among other molecules.

show abstract

“…Such results evidenced an important increase of TRKA gene among EOC as compared to HOSE cells and also an increase in MAPK1, PI3K, and AKT2, without changes in FOXL2 gene [4] (Figure 1).…”

Section: Ngf and Trka In Eocmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Role of nerve growth factor and its TRKA receptor in normal ovarian and epithelial ovarian cancer angiogenesis

Vera¹,

Tapia²,

Vega³

et al. 2014

Journal of Ovarian Research

View full text Add to dashboard Cite

show abstract

“…Interestingly, studies either have not found p-TRKA in normal tissues or it has only been found in a small number of samples [80]. Besides, NGF induces an increase of proliferation molecules and a decrease of apoptosis markers after NGF stimulation in EOC explants [81] (Figure 1). …”

Section: Neurotrophins and Their Role In Ovarian Cancermentioning

confidence: 99%

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer

Retamales-Ortega

Oróstica

Vera

et al. 2017

IJMS

View full text Add to dashboard Cite

Ovarian cancer is the eighth most common cancer in women worldwide, and epithelial ovarian cancer (EOC) represents 90% of cases. Nerve growth factor (NGF) and its high affinity receptor tyrosine kinase A receptor (TRKA) have been associated with the development of several types of cancer, including EOC; both NGF and TRKA levels are elevated in this pathology. EOC presents high angiogenesis and several molecules have been reported to induce this process. NGF increases angiogenesis through its TRKA receptor on endothelial cells, and by indirectly inducing vascular endothelial growth factor expression. Other molecules controlled by NGF include ciclooxigenase-2, disintegrin and metalloproteinase domain-containing protein 17 (ADAM17) and calreticulin (CRT), proteins involved in crucial processes needed for EOC progression. These molecules could be modified through microRNA regulation, which could be regulated by NGF. MicroRNAs are the widest family of non-coding RNAs; they bind to 3′-UTR of mRNAs to inhibit their translation, to deadenilate or to degraded them. In EOC, a deregulation in microRNA expression has been described, including alterations of miR-200 family, cluster-17-92, and miR-23b, among others. Since the NGF-microRNA relationship in pathologies has not been studied, this review proposes that some microRNAs could be associated with NGF/TRKA activation, modifying protein levels needed for EOC progression.

show abstract

“…The provoked canonical WNT/β-catenin signaling pathway can regulate the expression levels of a number of genes in bone diseases, cardiovascular diseases and cancers [36–39]. The emerging data have shown that NGF/NGFRs are overexpressed in ovarian cancer tissues and cells, but very low levels in normal ovarian tissues, which are correlated with the initiation, progression and prognosis of human ovarian cancers [25, 40]. And the WNT/β-catenin pathway plays an important role in carcinogenesis and development of all ovarian cancer subtypes [41–43].…”

Section: Introductionmentioning

confidence: 99%

Nerve growth factor modulates the tumor cells migration in ovarian cancer through the WNT/β-catenin pathway

Cai

Zhao

et al. 2016

Oncotarget

View full text Add to dashboard Cite

Nerve growth factor (NGF)/nerve growth factor receptors (NGFRs) axis and canonical WNT/β-catenin pathway have shown to play crucial roles in tumor initiation, progression and prognosis. But little did we know the relationship between them in modulation of tumor progress. In this report, we found that NGF/NGFRs and β-catenin were coexpression in ovarian cancer cell lines, and NGF can decrease the expression level of β-catenin and affect its activities, which may be related to the NGF-induced down-regulation of B-cell CLL/lymphoma 9-like (BCL9L, BCL9-2). Furthermore, NGF can also increase or decrease the downstream target gene expression levels of WNT/β-catenin depending on the cell types. Especially, we created a novel in vitro cell growth model based on a microfluidic device to intuitively observe the effects of NGF/NGFRs on the motility behaviors of ovarian cancer cells. The results showed that the migration area and maximum distance into three dimensional (3D) matrigel were decreased in CAOV3 and OVCAR3 cells, but increased in SKOV3 cells following the stimulation with NGF. In addition, we found that the cell colony area was down-regulated in CAOV3 cells, however, it was augmented in OVCAR3 cells after treatment with NGF. The inhibitors of NGF/NGFRs, such as Ro 08-2750, K252a and LM11A-31,can all block NGF-stimulated changes of gene expression or migratory behavior on ovarian cancer cells. The different results among ovarian cancer cells illustrated the heterogeneity and complexity of ovarian cancer. Collectively, our results suggested for the first time that NGF is functionally linked to β-catenin in the migration of human ovarian cancer cells, which may be a novel therapeutic perspective to prevent the spread of ovarian carcinomas by studying the interaction between NGF/NGFRs and canonical WNT/β-catenin signaling.

show abstract

Nerve Growth Factor Stimulates Cellular Proliferation of Human Epithelial Ovarian Cancer

Cited by 21 publications

References 26 publications

Role of nerve growth factor and its TRKA receptor in normal ovarian and epithelial ovarian cancer angiogenesis

Role of nerve growth factor and its TRKA receptor in normal ovarian and epithelial ovarian cancer angiogenesis

Role of Nerve Growth Factor (NGF) and miRNAs in Epithelial Ovarian Cancer

Nerve growth factor modulates the tumor cells migration in ovarian cancer through the WNT/β-catenin pathway

Contact Info

Product

Resources

About