Nerve Regeneration across a 2-cm Gap in the Rat Median Nerve Using a Resorbable Nerve Conduit Filled with Schwann Cells

Sinis, Nektarios; Schaller, Hans-Eberhard; Schulte-Eversum, Catarina; Schlosshauer, Bürkhard; Dosse, Michael; Dietz, Klaus; Roesner, Harald; Mueller, H.; Haerle, Max

doi:10.1055/s-2006-949709

Cited by 16 publications

(17 citation statements)

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“…Collagen has also demonstrated to increase nerve regeneration [301]. BD Matrigel ® and other lamini and collagen gels have been widely used as conduit fillings to incorporate or support cells and neurotrophic factors [302][303][304].…”

Section: Hydrogelsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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Section: Hydrogelsmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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“…More recently, research has been focused mainly on improving the single lumen nerve tube to bridge larger nerve gaps (de Ruiter, Malessy, Yaszemski, Windebank, & Spinner, 2009;de Ruiter, Spinner, Yaszemski, Windebank, & Malessy, 2009). The artificial conduit may be implanted empty, or it may be filled with collagen and laminin-containing gels (Labrador, Buti, & Navarro, 1998;Madison, Da Silva, & Dikkes, 1988;Verdu et al, 2002), internal frameworks (de Ruiter, Spinner et al, 2009;Francel, Francel, Mackinnon, & Hertl, 1997;Lundborg & Kanje, 1996;Meek et al, 2001;Nakamura et al, 2004;Yoshii & Oka, 2001;Yoshii, Oka, Shima, Taniguchi, & Akagi, 2003), supportive cells (Ansselin, Fink, & Davey, 1997;Evans et al, 2002;Guenard, Kleitman, Morrissey, Bunge, & Aebischer, 1992;Kim et al, 1994;Rodriguez, Verdu, Ceballos, & Navarro, 2000;Sinis et al, 2005), growth factors (Derby et al, 1993;Fine, Decosterd, Papaloizos, Zurn, & Aebischer, 2002;Hollowell, Villadiego, & Rich, 1990;Lee et al, 2003;Midha, Munro, Dalton, Tator, & Shoichet, 2003;Sterne, Brown, Green, & Terenghi, 1997), and conductive polymers, but combinations have also already been used (Figure 2). An artificial graft can meet many of the needs of regenerating fibres by concentrating neurotrophic factors, reducing cellular invasion and providing directional neuritis outgrowth to prevent neuroma formation.…”

Section: Nrg1 To Promote Nerve Repair Peripheral Nerve Injury and Repairmentioning

confidence: 99%

Neuregulin 1 Role in Schwann Cell Regulation and Potential Applications to Promote Peripheral Nerve Regeneration

Gambarotta

Fregnan

Gnavi

et al. 2013

International Review of Neurobiology

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Neuregulin 1 (NRG1) is a multifunctional and versatile protein: its numerous isoforms can signal in a paracrine, autocrine or juxtacrine manner, playing a fundamental role during the development of the peripheral nervous system and during the process of nerve repair, suggesting that the treatment with NRG1 could improve functional outcome following injury. Accordingly, the use of NRG1 in vivo has already yielded encouraging results.The aim of this review is to focus on the role played by the different NRG1 isoforms during peripheral nerve regeneration and remyelination and to identify good candidates to be used for the development of tissue engineered medical devices delivering NRG1, with the final goal to promote better nerve repair.

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“…These represent glial cells in the peripheral nervous system, and their main function is to provide support to the axons through the release of growth factors and isolation of the axon through formation of the myelin sheath (34) . The addition of SCs in synthetic tubes assists regeneration in nerve defects, although repair with autologous graft, in most studies, still presents superior recovery (27,29,33,(35)(36)(37) . Besides synthesizing growth factors, SCs also are able to produce extracellular molecules, such as laminin and type IV collagen.…”

Section: Schwann Cells In Nerve Repairmentioning

confidence: 99%

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Sebben¹,

Lichtenfels²,

Silva

2011

Rev. bras. ortop.

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Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for nerve repair. Among these, the tubulization technique can be highlighted: this allows regenerative factors to be introduced into the chamber. Cell therapy and tissue engineering have arisen as an alternative for stimulating and aiding peripheral nerve regeneration. Therefore, the aim of this review was to provide a survey and analysis on the results from experimental and clinical studies that used cell therapy and tissue engineering as tools for optimizing the regeneration process. The articles used came from the LILACS, Medline and SciELO scientific databases. Articles on the use of stem cells, Schwann cells, growth factors, collagen, laminin and platelet-rich plasma for peripheral nerve repair were summarized over the course Rev Bras Ortop. 2011;46(6):643-49 of the review. Based on these studies, it could be concluded that the use of stem cells derived from different sources presents promising results relating to nerve regeneration, because these cells have a capacity for neuronal differentiation, thus demonstrating effective functional results. The use of tubes containing bioactive elements with controlled release also optimizes the nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves. Another promising treatment is the use of platelet-rich plasma, which not only releases growth factors that are important in nerve repair, but also serves as a carrier for exogenous factors, thereby stimulating the proliferation of specific cells for peripheral nerve repair.

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Nerve Regeneration across a 2-cm Gap in the Rat Median Nerve Using a Resorbable Nerve Conduit Filled with Schwann Cells

Cited by 16 publications

References 0 publications

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Neuregulin 1 Role in Schwann Cell Regulation and Potential Applications to Promote Peripheral Nerve Regeneration

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

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