2015
DOI: 10.1371/journal.pgen.1005191
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Neto-Mediated Intracellular Interactions Shape Postsynaptic Composition at the Drosophila Neuromuscular Junction

Abstract: The molecular mechanisms controlling the subunit composition of glutamate receptors are crucial for the formation of neural circuits and for the long-term plasticity underlying learning and memory. Here we use the Drosophila neuromuscular junction (NMJ) to examine how specific receptor subtypes are recruited and stabilized at synaptic locations. In flies, clustering of ionotropic glutamate receptors (iGluRs) requires Neto (Neuropillin and Tolloid-like), a highly conserved auxiliary subunit that is essential fo… Show more

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Cited by 29 publications
(72 citation statements)
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“…Although a basic electrophysiological characterization of the adult VLM NMJ still needs to be done, I hypothesize that the smaller membrane apposition that has been found in this study is of functional importance and might be associated with different electrophysiological properties. In support of such a possibility is the observation that in neto mutants where the membrane apposition at synaptic sites was found to be smaller, there was a parallel reduction in GluRIIA levels and a reduction in the amplitude of spontaneous miniature excitatory postsynaptic potentials (mEPSPs), demonstrating that these morphological alterations correlate with a functional deficit that is consistent with altered GluRIIA abundance (Kim et al, ; Ramos et al, ). Interestingly, a similar phenotype has been described in hypomorphic gluRIIA mutants (Schmid et al, ).…”
Section: Discussionmentioning
confidence: 97%
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“…Although a basic electrophysiological characterization of the adult VLM NMJ still needs to be done, I hypothesize that the smaller membrane apposition that has been found in this study is of functional importance and might be associated with different electrophysiological properties. In support of such a possibility is the observation that in neto mutants where the membrane apposition at synaptic sites was found to be smaller, there was a parallel reduction in GluRIIA levels and a reduction in the amplitude of spontaneous miniature excitatory postsynaptic potentials (mEPSPs), demonstrating that these morphological alterations correlate with a functional deficit that is consistent with altered GluRIIA abundance (Kim et al, ; Ramos et al, ). Interestingly, a similar phenotype has been described in hypomorphic gluRIIA mutants (Schmid et al, ).…”
Section: Discussionmentioning
confidence: 97%
“…A properly formed membrane apposition seems to be essential for the correct targeting and maintenance of glutamate receptors (GluRs), a prerequisite for normal synaptic transmission. Mutations in neto (Neuropillin and Tolloid‐like), coding for an obligatory auxiliary subunit of the GluR complexes, are associated with alterations of membrane apposition formation and postsynaptic GluR accumulation (Kim, Bao, Bonanno, Zhang., & Serpe, 2012; Kim et al, ; Ramos, Igiesuorobo, Wang, & Serpe, ). Although a basic electrophysiological characterization of the adult VLM NMJ still needs to be done, I hypothesize that the smaller membrane apposition that has been found in this study is of functional importance and might be associated with different electrophysiological properties.…”
Section: Discussionmentioning
confidence: 99%
See 2 more Smart Citations
“…Primary antibodies from the Developmental Studies Hybridoma Bank were used at the following dilutions: mouse anti-GluRIIA (MH2B), 1:200; rat anti-Elav (7E8A10), 1:200. Other primary antibodies were as follows: rabbit anti-phosphorylated Mad (pMad), 1:500, (a gift from C. H. Heldin), rabbit anti-GluRIIC, 1:1000 (Ramos et al, 2015), Cy5-conjugated goat anti-HRP, 1:1000 (Jackson ImmunoResearch Laboratories, Inc.). Alexa Fluor 488-, Alexa Fluor 568-, and Alexa Fluor 647-conjugated secondary antibodies (Molecular Probes) were used at 1:400.…”
Section: Immunohistochemistrymentioning
confidence: 99%