Netrin 1 provides a chemoattractive cue for the ventral migration of GnRH neurons in the chick forebrain

Murakami, Shigeyuki; Ohki‐Hamazaki, Hiroko; Watanabe, Keisuke; Ikenaka, Kazuhiro; Ono, Katsuhiko

doi:10.1002/cne.22319

Cited by 15 publications

(13 citation statements)

References 67 publications

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“…Expression of tendon and myogenic markers were assessed with the following probes: cSCX (Schweitzer et al, 2001);cMYOD (Pourquié et al, 1996). The primers listed in Table S2 were used to generate probes detecting the following genes: cFHL1; cFZD1; cGDF6; cINHBA; cNTN1 (Murakami et al, 2010); cWNT4 (GEISHA ID, WNT4.UApcr); cWNT11 (GEISHA ID, WNT11.UApcr).…”

Section: In Situ Hybridization and Immunohistochemistrymentioning

confidence: 99%

Genome-wide strategies identify downstream target genes of chick connective tissue-associated transcription factors

et al. 2018

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Connective tissues support organs and play crucial roles in development, homeostasis and fibrosis, yet our understanding of their formation is still limited. To gain insight into the molecular mechanisms of connective tissue specification, we selected five zinc-finger transcription factors - OSR1, OSR2, EGR1, KLF2 and KLF4 - based on their expression patterns and/or known involvement in connective tissue subtype differentiation. RNA-seq and ChIP-seq profiling of chick limb micromass cultures revealed a set of common genes regulated by all five transcription factors, which we describe as a connective tissue core expression set. This common core was enriched with genes associated with axon guidance and myofibroblast signature, including fibrosis-related genes. In addition, each transcription factor regulated a specific set of signalling molecules and extracellular matrix components. This suggests a concept whereby local molecular niches can be created by the expression of specific transcription factors impinging on the specification of local microenvironments. The regulatory network established here identifies common and distinct molecular signatures of limb connective tissue subtypes, provides novel insight into the signalling pathways governing connective tissue specification, and serves as a resource for connective tissue development.

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Section: In Situ Hybridization and Immunohistochemistrymentioning

confidence: 99%

Genome-wide strategies identify downstream target genes of chick connective tissue-associated transcription factors

et al. 2018

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“…, Hoechst staining). To study neural differentiation, we analyzed the expression of neuron or neurite markers, such as Tuj1 (Kameda et al, ; Yang et al, ), neurofilament (Hatta et al, ; Murakami et al, ), and G4, which is homologous to L1 (Rathjen et al, ; Kuhn et al, ), after Pax3 overexpression (Fig. ).…”

Section: Inhibition Of Pax3 and Pax7 Expression By Specific Shrnas Domentioning

confidence: 99%

Pax3 and Pax7 interact reciprocally and regulate the expression of cadherin‐7 through inducing neuron differentiation in the developing chicken spinal cord

Lin

Wang

Yang

et al. 2015

J of Comparative Neurology

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Pax3 and Pax7 are closely related transcription factors that are widely expressed in the developing nervous system and somites. In the CNS, both genes are expressed in the dorsal part of the neural tube during development. Pax3 and Pax7 are involved in the sonic hedgehog (Shh) signaling pathway and are inhibited by Shh overexpression. The present study confirms in vivo that Pax3 overexpression represses the expression of Pax7, whereas Pax7 overexpression endogenously enhances and ectopically induces the expression of Pax3 in the developing chicken spinal cord. Overexpression of Pax3 and Pax7 represses the endogenous expression of cadherin-7, a member of the cadherin family of morphogenetic genes, and induces its ectopic expression. The present study also shows that overexpression of Pax3 and Pax7 changes the fate and morphology of cells in the neuroepithelial layer and induces the expression of postmitotic neuronal markers. We show that both Pax3 and Pax7 promote the differentiation of neural progenitor cells into neurons. Furthermore, the downregulation of Pax3 and Pax7 with specific shRNAs results in apoptosis in the developing spinal cord. Collectively, these results suggest that the transcription factors Pax3 and Pax7 play important roles in regulating morphogenesis and cell differentiation in the developing spinal cord.

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“…In the mouse, DCC has been reported to be essential for NETRIN-1 mediated attraction in many different systems 15 and DCC knockouts are not viable 14 . Previous in vitro studies have shown that in the chick as well, NETRIN-1 attracts sensory and motor neurons, spinal cord and hindbrain interneurons, GnRH neurons, enteric neural crest cells and oligodendrocyte precursors 20 21 23 49 50 51 52 53 . However, the conclusion of several previously published studies deserve to be reconsidered in the light of our results.…”

Section: Discussionmentioning

confidence: 99%

Recurrent DCC gene losses during bird evolution

Friocourt

Lafont

Kress

et al. 2017

Sci Rep

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During development, midline crossing by axons brings into play highly conserved families of receptors and ligands. The interaction between the secreted ligand Netrin-1 and its receptor Deleted in Colorectal Carcinoma (DCC) is thought to control midline attraction of crossing axons. Here, we studied the evolution of this ligand/receptor couple in birds taking advantage of a wealth of newly sequenced genomes. From phylogeny and synteny analyses we can infer that the DCC gene has been conserved in most extant bird species, while two independent events have led to its loss in two avian groups, passeriformes and galliformes. These convergent accidental gene loss events are likely related to chromosome Z rearrangement. We show, using whole-mount immunostaining and 3Disco clearing, that in the nervous system of all birds that have a DCC gene, DCC protein expression pattern is similar to other vertebrates. Surprisingly, we show that the early developmental pattern of commissural tracts is comparable in all birds, whether or not they have a DCC receptor. Interestingly, only 4 of the 5 genes encoding secreted netrins, the DCC ligands in vertebrates, were found in birds, but Netrin-5 was absent. Together, these results support a remarkable plasticity of commissural axon guidance mechanisms in birds.

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Netrin 1 provides a chemoattractive cue for the ventral migration of GnRH neurons in the chick forebrain

Cited by 15 publications

References 67 publications

Genome-wide strategies identify downstream target genes of chick connective tissue-associated transcription factors

Genome-wide strategies identify downstream target genes of chick connective tissue-associated transcription factors

Pax3 and Pax7 interact reciprocally and regulate the expression of cadherin‐7 through inducing neuron differentiation in the developing chicken spinal cord

Recurrent DCC gene losses during bird evolution

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