Network medicine might lead to new treatments for dyslipidemia. It will be a challenging method to implement in a clinical context

2021

Preprint

Global Initiative for Chronic Obstructive Lung Disease (GOLD) research on chronic obstructive pulmonary condition (COPD) characterizes it as a prevalent, preventable, and curable illness with persistent respiratory symptoms and airflow limitation. airway blockage resulting in dyspnoea and air trapping when exercising airflow restriction can be attributed to smoking, ambient tobacco smoke exposure, and the patient's TB history COPD airflow restriction is largely caused by airway tightness, chronic bronchitis, or emphysema. Repeated inhalation of hazardous particles induces chronic inflammatory immune cell infiltration, tissue repair, and airway remodeling, resulting in a 4-to 40-fold increase in airway resistance and bronchiolar constriction. 20% of small airways are bronchi, with the rest being either bronchioles or alveolar ductal gaps. Flow restriction includes alveolar wall disintegration and alveolar support. The modifications worsen the rapid FEV1 fall and contribute to the airway obstruction seen in COPD. COPD, small airway disease (SAD), has been extensively studied. Inflammation, fibrosis, and destruction of bronchioles are all indications of this illness. mixed pulmonary fibrosis and emphysema (CPFE). In fact, fibrosis appears to be involved in the obstruction of small airways.RAGE participates in several intracellular processes by binding to several ligands. RAGE ligand interactions trigger downstream signaling pathways that have been associated with COPD and other disorders. Thus, inhibitors of RAGE and its signaling pathways also have been found to play a function in other disorders, which suggests that they might be used to treat COPD. There are currently no established biomarkers to diagnose COPD. COPD patients are shown to have altered RAGE and some of its ligands. Despite a lack of agreement on how smoking affects sRAGE levels, greater study is needed to discover whether or not this biomarker is stable. It was discovered that among the North Han Chinese,-429T > C is associated with COPD. COPD vulnerability is furthermore associated with the genetics of RAGE. To get a better diagnosis, population-based studies are needed. Finally, RAGE, AGE-RAGE, and sRAGE all impact COPD, and RAGE may be employed as a biomarker for diagnosis and management of COPD patients if more study supports this hypothesis.

Section: Discussionmentioning

confidence: 99%

Chronic obstructive pulmonary condition (COPD) is a prevalent, preventable, and curable illness with persistent respiratory symptoms and airflow limitation

2021

Preprint

“…Transfection of protoplasts can be employed for both temporary and stable editing. The techniques of gene transfer utilized in plant gene editing (121,122,123). A. rhizogenes is specially employed to evaluate editing efficiency in plant root hairs for root hair transformation, and this approach has mostly been employed in legume species like Medicago and soybean (124,125,126).…”

Section: Delivery Based On Proteinsmentioning

confidence: 99%

CRISPR/Cas system research has advanced significantly in biological sciences.There are still many challenges to effective delivery before efficient gene editing may be achieved

2021

Preprint

The CRISPR/Cas system's discovery and execution offer great promise for the treatment of human illnesses and the revolutionization of plant breeding. Despite the fact that CRISPR/Cas system research is well advanced in the life sciences community, there are still considerable barriers to effective delivery that must be overcome before effective gene editing can be achieved. Consider characteristics like specificity, efficacy, and controlled expression while deciding on a plan. Due to the discovery of innovative delivery systems, many of the shortcomings of the CRISPR/Cas system's translatability have been addressed. Because of the rapid growth of delivery mechanisms, a successful translation of CRISPR/Cas technology into medical and agricultural applications is vital, and substantial progress is predicted.

“…In fact, they stymie this transition by stymieing a variety of E2F gene targets and cell cycle promoters. (122) Senescence is a very diverse process with various regulators and elements involved in its onset, maintenance, and regulation. Because miRNAs may alter several genes and pathways, they can effectively regulate senescence, perhaps acting as a senescence promoter.…”

Section: Targeting Senescencementioning

confidence: 99%

Exosomal miRNA diagnostic and gene therapy tools

2021

Preprint

The field of miRNA-based therapies is constantly expanding as a result of substantial research conducted across the world. Exosomal origin must be addressed for the use of exosomal miRNA in cancer diagnostics. A uniform procedure for exosome separation and detection should be devised, as current approaches have various limitations. More research on maximizing the benefits of target variety while avoiding off-target impacts is needed. miRNAs are involved in a number of cancer-related pathways, as well as developmental and regulatory processes. miRNAs control a large number of genes. The possibility of miRNA treatments having an off-target impact is a serious worry. Various techniques, including viral, nonviral, and chemical alterations, are recommended to improve target delivery. Nanoparticle-based delivery is being studied extensively, and attempts are being made to reduce toxicity and cellular accumulation. Next-generation sequencing of miRNAs is being used to study the functions that miRNA can play as a biomarker for diagnosis, detection, and prognosis. Several miRNA signatures unique to cancer types have evolved, with some of them now being tested in therapeutic studies. Antisense oligonucleotides that block miRNIs, tumor and CSC-targeted nanoparticle treatment, and combination treatment with chemotherapeutic agents are all promising clinical strategies for cancer personalized medicine.