Network Pharmacology-Based Prediction and Verification of the Targets and Mechanism for Panax Notoginseng Saponins against Coronary Heart Disease

Abstract

Coronary heart disease (CHD) is the worldwide leading cause for cardiovascular death. Panax notoginseng saponin (PNS), which is the main bioactive compound of panax notoginseng, has been generally accepted to exert a remarkable effect on CHD for a long time. However, to reveal the underlying treatment target and corresponding mechanism of PNS against CHD is still a substantial challenge. In this work, the targets and mechanism of PNS against CHD were successfully achieved by pharmacology-based prediction and experimental verification. 36 common targets were screened out through integrating the gene expression profile of CHD and the chemical-protein data of PNS. Then, two key nodes were further selected for verification by experiment after analyzing GO function, KEGG pathway, coexpression, and topology analysis. Results showed that PNS has protected the human umbilical vein endothelial cells from H2O2-induced oxidative stress by inhibiting early cell apoptosis via upregulating VEGFA mRNA expression. Therefore, our research has successfully pointed out one treatment target and apoptotic inhibition caused by PNS with method of integrating bioinformatics prediction and experimental verification, which has partially explained the pharmacological mechanism of PNS against CHD.

Saponins as Modulators of the Blood Coagulation System and Perspectives Regarding Their Use in the Prevention of Venous Thromboembolic Incidents

“…Zhang et al also note that saponins isolated from the roots and rhizomes of P. notoginseng may play an important function in treating cardiovascular diseases such as coronary heart diseases, cardiac arrhythmia and angina pectoris [79]. In addition, Dong et al found P. notoginseng saponins to protect human umbilical vein endothelial cells from H 2 O 2 -stimulated oxidative stress [14].…”

“…root: This extract was shown to alleviate chronic doxorubicin-induced heart failure in an animal model by inhibiting oxidative stress [12]. Sun et al and Dong et al also report Panax notoginseng saponins to demonstrate antioxidative activity [13,14]. However, the chemical structure, and hence the properties of saponins, have been found to change during storage, and under other conditions [7].…”

Mechanism of Dayuanyin in the treatment of coronavirus disease 2019 based on network pharmacology and molecular docking

“…OB and DL property are important reference standards for evaluating whether compounds can be used as drugs. In this study, OB ≥ 30% and DL ≥ 0.18 were used as screening thresholds [14]. According to the selected active ingredients of DYY, the target genes corresponding to the above active ingredients derived from DrugBank were further screened using Perl language in combination with the TCMSP database.…”

Phytotherapie bei koronarer Herzkrankheit – Was gibt es an Evidenz?

“…In der Traditionellen Chinesischen Medizin wurde Ginseng bzw. die Wurzel aus Panax notoginseng als "Anti-Aging-Droge" zur Behandlung verschiedener altersbedingter Krankheiten, darunter auch der Angina pectoris und der koronaren Herzkrankheit, verwendet [ [28].…”