Neural crest stem cells: discovery, properties and potential for therapy

Achilleos, Annita; Trainor, Paul A.

doi:10.1038/cr.2012.11

Cited by 263 publications

(217 citation statements)

References 109 publications

(178 reference statements)

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“…We therefore hypothesized that SHH signaling-associated cleft lip occurred via disruption of WNT-mediated p63/IRF6 signaling. Via in situ hybridization, we found p63/ IRF6 signaling noticeably inhibited at the lambdoidal junction in cells (CNCs) play critical roles in producing cartilage, bone, and connective tissues as well as regulating facial patterning (26)(27)(28). SHH signaling has been shown to be a critical regulator of CNC development (29).…”

mentioning

confidence: 99%

Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis

Kurosaka¹,

Iulianella²,

Williams³

et al. 2014

J. Clin. Invest.

108

128

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Cleft lip, which results from impaired facial process growth and fusion, is one of the most common cranio facial birth defects. Many genes are known to be involved in the etiology of this disorder; however, our under standing of cleft lip pathogenesis remains incomplete. In the present study, we uncovered a role for sonic hedgehog (SHH) signaling during lip fusion. Mice carrying compound mutations in hedgehog acyltransferase (Hhat) and patched1 (Ptch1) exhibited perturbations in the SHH gradient during frontonasal development, which led to hypoplastic nasal process outgrowth, epithelial seam persistence, and cleft lip. Further investi gation revealed that enhanced SHH signaling restricts canonical WNT signaling in the lambdoidal region by promoting expression of genes encoding WNT inhibitors. Moreover, reduction of canonical WNT signaling perturbed p63/interferon regulatory factor 6 (p63/IRF6) signaling, resulting in increased proliferation and decreased cell death, which was followed by persistence of the epithelial seam and cleft lip. Consistent with our results, mutations in genes that disrupt SHH and WNT signaling have been identified in both mice and humans with cleft lip. Collectively, our data illustrate that altered SHH signaling contributes to the etiology and pathogenesis of cleft lip through antagonistic interactions with other gene regulatory networks, including the canonical WNT and p63/IRF6 signaling pathways.

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mentioning

confidence: 99%

Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis

Kurosaka¹,

Iulianella²,

Williams³

et al. 2014

J. Clin. Invest.

108

128

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show abstract

“…Along the way, the field of stem cell biology has naturally become an important research area covered by papers published in Cell Research [2]. For instance, in the past year of 2012, Cell Research has published a number of important papers related to the stem cell field, covering diverse aspects and topics such as induced pluripotent stem (iPS) cells [3][4][5][6][7], mechanistic studies of pluripotency and differentiation [8][9][10][11], modeling of human diseases using stem cell-based systems [6,12], direct reprogramming of somatic cells to other cell types without passing through an pluripotent intermediate [13][14][15][16], as well as neural crest stem cells [17] and cancer stem cells [18][19][20].…”

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confidence: 99%

The 2013 special issue on stem cell biology

2013

Cell Res

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“…Given the identity of SCs, the neural crest stem cells medium was considered and adapted to SCs culture through addition of chemically defined ingredients. (Achilleos and Trainor 2012;Li et al 2007;Martin et al 2012). Ingredients such as bFGF and EGF are common mitogens for almost all kinds of cells including SCs (Monje et al 2009).…”

Section: Discussionmentioning

confidence: 99%

An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves

et al. 2015

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Schwann cells (SCs), the supporting cells of the peripheral nerves, are indispensable for regenerating the peripheral and central nervous system. Copious preparation of these cells in a well-defined manner is to be a privileged position. SCs cultivation is overwhelmed by contaminating fibroblasts which are often outgrowing as the predominant cell type in an in vitro culture. This study introduces a technically simple and efficient procedure for SCs isolation and enrichment based on implementing recombinant and defined supplements.Collected adult rat sciatic nerves were cultured for 10 days as in vitro predegeneration. After dissociation and plating, the medium changed to knockout serum replacement supplemented DMDM/F12 medium containing various growth factors. The whole procedure took 3 weeks and SCs purity was then evaluated through implementing specific cytoplasmic and membranous markers. The viability of enriched SCs were evaluated by MTT assay. Within 10 days, over 99 % homogenous SCs were achieved and confirmed through immunofluorescence staining and flow-cytometry for P75 NTR and S100 markers, respectively. MTT data revealed that the viability and metabolic activities of purified SCs were increased in expansion medium. This study provides a technically easy and efficient method with the benefits of not utilizing bovine serum or other animal products for SCs isolation and enrichment.

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Neural crest stem cells: discovery, properties and potential for therapy

Cited by 263 publications

References 109 publications

Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis

Disrupting hedgehog and WNT signaling interactions promotes cleft lip pathogenesis

The 2013 special issue on stem cell biology

An efficient system for selection and culture of Schwann cells from adult rat peripheral nerves

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