Neural stem cell conditioned medium alleviates Aβ&lt;sub&gt;25-35&lt;/sub&gt; damage to SH-SY5Y cells through the PCMT1/MST1 pathway

Jia, Guoyong; Yang, Hongna; Diao, Zengyan; Liu, Ying; Sun, Congcong

doi:10.4081/ejh.2020.3135

Cited by 2 publications

(2 citation statements)

References 26 publications

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“…The concentration of each material was set based on the concentration that did not affect cell survival. The methods were performed as previously described [ 99 ].…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Effect and Antioxidant Potency of Fermented Cultured Wild Ginseng Root Extracts of Panax ginseng C.A. Meyer in Mice

Kim¹,

Ryu

Lee

et al. 2021

Molecules

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Wild ginseng has better pharmacological effects than cultivated ginseng. However, its industrialization is limited by the inability to grow wild ginseng on a large scale. Herein, we demonstrate how to optimize ginseng production through cultivation, and how to enhance the concentrations of specific ginsenosides through fermentation. In the study, we also evaluated the ability of fermented cultured wild ginseng root extract (HLJG0701-β) to inhibit acetylcholinesterase (AChE), as well as its neuroprotective effects and antioxidant activity. In in vitro tests, HLJG0701-β inhibited AChE activity and exerted neuroprotective and antioxidant effects (showing increased catalyst activity but decreased reactive oxygen species concentration). In in vivo tests, after HLJG0701-β was orally administered at doses of 0, 125, 250, and 500 mg/kg in an animal model of memory impairment, behavioral evaluation (Morris water maze test and Y-maze task test) was performed. The levels of AChE, acetylcholine (ACh), blood catalase (CAT), and malondialdehyde (MDA) in brain tissues were measured. The results showed that HLJG0701-β produced the best results at a dose of 250 mg/kg or more. The neuroprotective mechanism of HLJG0701-β was determined to involve the inhibition of AChE activity and a decrease in oxidative stress. In summary, both in vitro and in vivo tests confirmed that HJG0701-β administration can lead to memory improvement.

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“…The concentration of each material was set based on the concentration that did not affect cell survival. The methods were performed as previously described [ 99 ].…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Effect and Antioxidant Potency of Fermented Cultured Wild Ginseng Root Extracts of Panax ginseng C.A. Meyer in Mice

Kim¹,

Ryu

Lee

et al. 2021

Molecules

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“…Obviously, the detected influence of conditioned media from 24-h cultures of rat FBNCs (E14-16), most of which are NSCs/NPCs, on the endogenous regeneration in the culture of neural cells of rat fetal brain with a mechanical model of neurotrauma in vitro is based on the cumulative effect of biologically active paracrine mediators (signaling molecules of secretome) produced by these cells in the culture medium [11,20,22,[37][38][39][40]. Secreted mediators are responsible for one of the main mechanisms of NSCs/NPCs effects, which means modulation of the local microenvironment around the affected area and activation of key inflammatory and regenerative programs in the CNS [11,14,21], i.e.…”

Section: Morphometric Study Of Morphological Changes In Neural Cell Culture With a Mechanical Model Of Neurotrauma Under Different Cultivmentioning

confidence: 99%

Dynamics of morphological changes in neural cell culture with a model of neurotrauma under the influence of conditioned media of the rat fetal brain neurogenic cells

Pedachenko¹,

Любич²,

Стайно³

et al. 2020

COT

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A potential strategy for recovery and regeneration of brain damage due to traumatic brain injury is considered to be the transplantation of neurogenic stem and/or progenitor cells (NSCs/NPCs). The key factors of the regenerative non-targeted effects of NSCs/NPCs (so-called bystander effects) include the signal molecules produced by them into the extracellular environment (secretome). The purpose is to study the regenerative bystander effects of rat fetal brain neurogenic cells (FBNCs) in the in vitro model of neurotrauma. Materials and methods. In cell culture of FBNCs from rat fetuses (E14-16), neurotrauma was modeled in vitro by mechanical scratching of monolayer and conditioned medium obtained from 24-h cultures of rat FBNCs was added. Cell phenotype was evaluated by morphological features and by immunocytochemical staining for Nestin and GFAP. The density and length of processes, migration capacity, the cell growth rate and monolayer density in the scratched area were compared. Morphometric study included analysis of the width of the scratched area, the number of migrating cells, the distance of migration and mitotic activity in the intact monolayer. Results. Under the conditions of the nutrient medium of standard composition in the scratched area the signs of endogenous regeneration are shown during 24-48 h of cultivation. The overgrowth of cell processes from monolayer and short distance migration of single undifferentiated or poorly differentiated cells were shown. In the next 72-96 h of observation, the degeneration of migrated cells and processes in the scratched area was detected. Under the influence of conditioned media from 24-h cultures of FBNCs by single addition immediately after scratching at dose of 0.1 mg/ml for protein content the stimulation of regeneration were detected up to 96 hours of cultivation. The migration of cell processes from the monolayer simultaneously with undifferentiated or poorly differentiated cells at 24 hours was shown. The formation of cell clusters and their differentiation (at 48 h), as well as migration of differentiated cells with partial or complete overgrowth of scratched area (72-96 h) were observed. The morphological signs of degeneration of migrated cells in the scratched area appeared only on the 8th day of cultivation. Conditioned media does not affect qualitative and quantitative properties of the culture of rat FBNCs in the intact area where mitotic activity was average. Conclusions. Conditioned medium from 24-h cultures of rat FBNC can stimulate reparation in the in vitro model of neurotrauma in neural cell culture for at least 7 days at a single addition, without affecting the cellular composition and mitotic activity of the intact monolayer.

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Neural stem cell conditioned medium alleviates Aβ<sub>25-35</sub> damage to SH-SY5Y cells through the PCMT1/MST1 pathway

Cited by 2 publications

References 26 publications

Neuroprotective Effect and Antioxidant Potency of Fermented Cultured Wild Ginseng Root Extracts of Panax ginseng C.A. Meyer in Mice

Neuroprotective Effect and Antioxidant Potency of Fermented Cultured Wild Ginseng Root Extracts of Panax ginseng C.A. Meyer in Mice

Dynamics of morphological changes in neural cell culture with a model of neurotrauma under the influence of conditioned media of the rat fetal brain neurogenic cells

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