Neural stem cell markers, nestin and musashi proteins, in the progression of human glioma: correlation of nestin with prognosis of patient survival

Strojnik, Tadej; Røsland, Gro Vatne; Sakariassen, Per Øystein; Kavalar, Rajko; Lah, Tamara T.

doi:10.1016/j.surneu.2006.10.050

Cited by 204 publications

(187 citation statements)

References 36 publications

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“…1,2,[31][32][33][34] In glial tumors, increased numbers of tumor cells expressing stem cell-associated markers such as AC133 and the intermediate filament nestin are associated with poor patient survival and earlier tumor recurrence. 28,35 Bearing in mind the aberrant expression levels of molecules involved in RA availability, as well as the increased intratumoral retinoid levels described in the present study (CRBP1, ALDH1A1), these seemingly contrary observations raise the intriguing question of whether undifferentiated glioma cells can escape physiological antitumor influences of RA. 18 In support of this notion, we observed a strong correlation between expression of CRBP1 and tumor cell proliferation, between CRBP1 and the stem cell-related transcription factor SOX2, and between FABP5 and the stem cell-associated protein nestin.…”

Section: Discussioncontrasting

confidence: 51%

Aberrant Expression of Retinoic Acid Signaling Molecules Influences Patient Survival in Astrocytic Gliomas

Campos

Centner

Bermejo

et al. 2011

The American Journal of Pathology

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Undifferentiated cell populations may influence tumor growth in malignant glioma. We investigated potential disruptions in the retinoic acid (RA) differentiation pathway that could lead to a loss of differentiation capacity, influencing patient prognosis. Expression of key molecules belonging to the RA differentiation pathway was analyzed in 283 astrocytic gliomas and was correlated with tumor proliferation, tumor differentiation, and patient survival. In addition, in situ concentrations of retinoids were measured in tumors, and RA signaling events were studied in vitro. Unlike other tumors, in gliomas expression of most RA signaling molecules increased with malignancy and was associated with augmented intratumoral retinoid levels in high-grade gliomas. Aberrantly expressed RA signaling molecules included i) the retinol-binding protein CRBP1, which facilitates cellular retinoid uptake; ii) ALDH1A1, capable of activating RA precursors; iii) the RA-degrading enzyme CYP26B1; and iv) the RA-binding protein FABP5, which can inhibit RA-induced differentiation. In contrast, expression of the RA-binding protein CRABP2, which fosters differentiation, was decreased in high-grade tumors. Moreover, expression of CRBP1 correlated with tumor proliferation, and FABP5 expression correlated with an undifferentiated tumor phenotype. CRBP1 and ALDH1A1 were independent prognostic markers for adverse patient survival. Our data indicate a complex and clinically relevant deregulation of RA signaling, which seems to be a central event in glioma pathogenesis.

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Section: Discussioncontrasting

confidence: 51%

Aberrant Expression of Retinoic Acid Signaling Molecules Influences Patient Survival in Astrocytic Gliomas

Campos

Centner

Bermejo

et al. 2011

The American Journal of Pathology

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“…Nestin has been detected in brain tumors such as pilocytic astrocytomas and malignant gliomas including glioblastoma multiforme (27,28,52,53). Our results showed that STM91-01, TTC549 and TTC1240 expressed nestin, which was detected by RT-PCR and Western blotting.…”

Section: Discussionmentioning

confidence: 53%

“…Msi-1 is an RNA binding protein which is preferentially expressed in stem-cells, functions as a translational repressor (56)(57)(58), and is widely used as a molecular marker to identify stem cells in embryos and adult tissues (26)(27)(28)(29). In our study, Msi-1 was expressed in five MRT cell lines except TTC642, which originated from a neck mass.…”

Section: Discussionmentioning

confidence: 69%

“…In recent years, the concept of CSC involvement in tumorigenesis has attracted the attention of many researchers. Previous studies have shown that NSC acting as CSC is recognized in CNS (24)(25)(26)(27)(28)(29). In the present study, we examined the expression of NSC markers such as CD133, nestin and Msi-1 before and after 4-HPR induced differentiation in MRT cell lines to elucidate the cytological characteristics of MRT cells that are considered to derive from neuroectodermal origin (8,9,12).…”

Section: Introductionmentioning

confidence: 95%

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Expression of neural stem cell markers in malignant rhabdoid tumor cell lines

Okuno

Ohta²,

Katô³

et al. 2009

Oncol Rep

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Abstract. Malignant rhabdoid tumor (MRT) is considered to display multi-phenotypic characteristics but the true origin of this tumor remains unknown. In recent years, the concept of the cancer stem cell (CSC) has drawn great attention. In the present study we investigated six MRT cell lines (TM87-16, STM91-01, TTC642, TTC549, YAM-RTK-1 and TTC1240), for CD133, nestin and Musashi-1 (Msi-1), which are considered to be CSC as well as neural stem cell (NSC) markers, using assays for cell viability and apoptosis, reverse transcriptional polymerase chain reaction (RT-PCR), semiquantitative PCR and Western blot analysis before and after differentiation-induction with N-(4-hydroxyphenyl) retinamid (4-HPR). Before differentiation-induction with 4-HPR, CD133 was detected in three MRT cell lines, nestin in three cell lines and Msi-1 in five cell lines. In TTC549 after differentiation-induction with 4-HPR, nestin and Msi-1 were down-regulated in a time-dependent manner. Similar downregulation of Msi-1 was recognized in YAM-RTK-1. In STM91-01, CD133 was gradually down-regulated and Msi-1 was down-regulated after a transient increase. Results from our study indicated that 4-HPR might be effective in some MRTs. Expression of NSC markers showed that some MRTs contain a subpopulation of NSC and down-regulation of NSC markers in MRT cells provides supportive evidence that many MRTs could be considered of neuroectodermal origin.

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“…[1][2][3] Nestin is also expressed by many other stem cells and it is considered a primitive marker. [4][5][6][7] Upon differentiation, Nestin becomes downregulated and is replaced by tissue-specific intermediate filament proteins. 8 During neuro-and gliogenesis, Nestin is replaced by cell type-specific intermediate filaments, e.g.…”

Section: Introductionmentioning

confidence: 99%

Nestin, an Important Marker for Differentiating Oligodendroglioma from Astrocytic Tumors

Abu-Farsakh¹

2017

ICPJL

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Background: Nestin is an acronym for neuroepithelial stem cell protein. It is an intermediate filament protein expressed in proliferating cells during the developmental stages in a variety of embryonic and fetal tissues. It is also expressed in some adult stem/progenitor cell populations, such as newborn vascular endothelial cell. Differentiation between astrocytic tumors and oligodenoglioma tumor is of paramount importance because of different lines of treatment and different prognosis.Design: We performed Nestin immunostaining on paraffin blocks of 16cases of astrocytomas of various grades (3Glioblastoma, 3anaplastic astrocytoma, 3fibrillary astrocytoma and 7Pilocytic astrocytoma) and on 12 oligodendroglioma (6 grade II, and 6 grade III). All cases of oligodendroglioma has confirmation by FISH for 1p 19q.Result: Nestin staining was seen in all astrocytic tumors. The strongest staining was in glioblastomas and in anaplastic astrocytomas. Pilocytic astrocytomas show mostly focal and weak staining with strong staining of Rosenthal fibers. Grade II astrocytoma shows weak but more intense staining than pilocytic astrocytoma. No Nestin immunostaining was seen in any of the oligodendroglioma tumor cells, but Nestin stained the endothelial cells in oligodendroglioma as a positive internal control.

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Neural stem cell markers, nestin and musashi proteins, in the progression of human glioma: correlation of nestin with prognosis of patient survival

Cited by 204 publications

References 36 publications

Aberrant Expression of Retinoic Acid Signaling Molecules Influences Patient Survival in Astrocytic Gliomas

Aberrant Expression of Retinoic Acid Signaling Molecules Influences Patient Survival in Astrocytic Gliomas

Expression of neural stem cell markers in malignant rhabdoid tumor cell lines

Nestin, an Important Marker for Differentiating Oligodendroglioma from Astrocytic Tumors

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