Neuregulin 1 discovered as a cleavage target for the HCV NS3/4A protease by a microfluidic membrane protein array

Schwartz, Nika; Pellach, Michal; Glick, Yaïr; Gil, Reuven; Levy, Gahl; Avrahami, Dorit; Barbiro-Michaely, Efrat; Nahmias, Yaakov; Gerber, Doron

doi:10.1016/j.nbt.2018.02.004

Cited by 2 publications

(1 citation statement)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the next research step was focused on the change of cell membrane structure. As the boundary membrane of cells, cell membrane integrity is important to maintain the normal activities of cells [25] , such as material transport [26], energy conversion [27] , and cell recognition [28]. When the membrane was damaged, LDH, a relatively stable cytoplasmic enzyme, was released to the outside.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Liver Toxicity of Pterocephalus hookeri Extract via Triggering Necrosis

Wang

Dong

Zhang

et al. 2019

Toxins

View full text Add to dashboard Cite

Pterocephalus hookeri (C. B. Clarke) Höeck, recorded in the Chinese Pharmacopoeia (2015 version) as a Tibetan medicine for the treatment of various diseases, especially rheumatoid arthritis, was believed to possess a slight toxicity. However, hardly any research has been carried out about it. The present study aimed to evaluate the toxicity in vivo and in vitro. Toxicity was observed by the evaluation of mice weight loss and histopathological changes in the liver. Then, the comparison research between ethyl acetate extract (EAE) and n-butanol extract (BUE) suggested that liver toxicity was mainly induced by BUE. The mechanical study suggested that BUE-induced liver toxicity was closely associated with necrosis detected by MTT and propidium iodide (PI) staining, via releasing lactate dehydrogenase (LDH), reducing the fluidity, and increasing the permeability of the cell membrane. Western blot analysis confirmed that the necrosis occurred molecularly by the up-regulation of receptor-interacting protein kinase 1 (RIP1) and receptor-interacting protein kinase 3 (RIP3), as well as the activation of the nuclear factor-kappa-gene binding (NF-κB) signaling pathway in vivo and in vitro. This finding indicated that the liver toxicity induced by BUE from P. hookeri was mainly caused by necrosis, which provides an important theoretical support for further evaluation of the safety of this folk medicine.

show abstract