2018
DOI: 10.3389/fnbeh.2018.00316
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Neuroanatomy- and Pathology-Based Functional Examinations of Experimental Stroke in Rats: Development and Validation of a New Behavioral Scoring System

Abstract: In experimental stroke studies, a neuroanatomy-based functional examination of behaviors is critical to predict the pathological extent of infarcts because brain-imaging studies are not always available. However, there is a lack of systematic studies to examine the efficiency of a behavioral test for this purpose. Our work aimed to design a new score for this goal in stroke rats, by simplifying the Garcia score (with subscore 1–6) and adding circling as subscore 7. MRI and 2,3,5-triphenyltetrazolium chloride s… Show more

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Cited by 7 publications
(4 citation statements)
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“…All neurobehavioral tests were performed blinded. The modified Garcia Score table with expanded descriptions for each component 68,89 :…”
Section: Methodsmentioning
confidence: 99%
“…All neurobehavioral tests were performed blinded. The modified Garcia Score table with expanded descriptions for each component 68,89 :…”
Section: Methodsmentioning
confidence: 99%
“…Permanent and transient MCAO (pMCAO or tMCAO) procedures were performed on rats to mimic human ischemic stroke without and with recanalization, respectively. The MCAO model was induced by occlusion of the right middle cerebral artery using a silicon-coated nylon filament (MCAO sutures, RWD Life Science, Shenzhen, China), as previously described ( Yeh et al, 2018 ). The filament was not removed in the pMCAO group and was removed after 90 and 30 min in the tMCAO-90 and tMCAO-30 groups, respectively.…”
Section: Methodsmentioning
confidence: 99%
“…Brain MRI was performed at 3 h after pMCAO, tMCAO, or sham operation ( n = 29; 4 for proteomics experiment and 25 for immunohistochemical study) using 7T MRI machine (Bruker PharmaScan, Ettlingen, Germany) at National Taiwan University to acquire the T2-weighted images (T2) and DWI ( Yeh et al, 2018 ). After MRI acquisition, the brain for proteomics experiment was harvested following intracardiac perfusion with 1% sodium nitrite under anesthesia.…”
Section: Methodsmentioning
confidence: 99%
“…The extracted brain and hemispheres are crucial to subsequent processes, such as registration, warping, classification, identification, and volume measurement 9 . Due to the lack of appropriate computer‐aided tools, manual delineation of the brain and hemisphere regions on numerous images has been traditionally adopted for small animal studies, 3,10,11 which is a time‐consuming and labor‐intensive task. Consequently, accurate and robust image segmentation tools for extracting the brains and hemispheres in MR images are critical in experimental stroke rat analysis 12 …”
Section: Introductionmentioning
confidence: 99%