Neurogenic cells in human amniotic fluid

Prusa, Andrea‐Romana; Márton, Erika; Rosner, Margit; Bettelheim, Dieter; Lubec, Gert; Pollack, Arnold; Bernaschek, Gerhard; Hengstschläger, Markus

doi:10.1016/j.ajog.2003.12.014

Cited by 129 publications

(86 citation statements)

References 13 publications

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“…Amniotic fluid is known to contain multiple cell types derived from the developing fetus (Priest et al, 1978;Polgá r et al, 1989). Cells within this heterogeneous population can give rise to diverse differentiated cells including those of adipose, muscle, bone and neuronal lineages (In 't Anker et al, 2003;Prusa et al, 2004;Tsai et al, 2004). Human AFS cells can give rise to neurogenic, adipogenic, osteogenic, myogenic and endothelial lineages, inclusive of all embryonic germ layers (De Coppi et al, 2007).…”

Section: R E T R a C T E Dmentioning

confidence: 99%

Development of cloned embryos from porcine neural stem cells and amniotic fluid-derived stem cells

Zhao

Zheng

2010

Animal

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The aim of this study was to determine the developmental ability of cloned embryos derived from porcine neural stem (NS) cells, amniotic fluid-derived stem (AFS) cells, differentiated cells from NS and AFS cells, fetal fibroblast (FF) cells, adult fibroblast (AF) cells and mammary gland epithelial (MGE) cells. NS, AFS and FF cells were isolated from embryonic day 30 porcine fetus, AF and MGE cells were isolated from adult pig. NS and AFS cells were induced to differentiate into different cell types, respectively. Stem cells and their differentiated cells were harvested for analysis of the markers using reverse transcription PCR. NS and AFS cells, their differentiated cells, FF, AF and MGE cells were used for nuclear transfer, respectively. A total of 100 two-cell stage cloned embryos derived from each cell line were transferred into the oviducts of surrogate mothers. The results showed that the neurospheres were positive for the undifferentiated neural cell marker, Nestin and NS cells widely expressed NogoA, DCX, CyclinD2, CD133, Hes1, Oct4, Desmin, CD-90, Nanog and Sox2. AFS cells widely expressed NogoA, DCX, CyclinD2, CD133, Hes1, Nanog, Sox2, Oct4, Desmin and CD-90. Both NS and AFS cells were differentiated into astrocyte (GFAP 1 ), oligodendrocyte (GalC 1 ), neuron (NF

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Section: R E T R a C T E Dmentioning

confidence: 99%

Development of cloned embryos from porcine neural stem cells and amniotic fluid-derived stem cells

Zhao

Zheng

2010

Animal

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“…In addition these cells can survive after implantation into a rat model of Parkinson's disease [18] or after spinal cord injury [19] and differentiate into neuron-like cells after transplantation [9]. Only a recent article by Prusa et al reports neuronal differentiation of AFCs after exposition to a medium containing 2% serum and 1.25% dimethyl sulfoxide (DMSO) [20]. This effect could be induced by the presence of DMSO in their culture, which can cause cell shrinkage and neuron-like appearance as reported by some authors [21,22].…”

Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

et al. 2006

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“…AFSCs have been shown to harbor the potential for osteogenic, chondrogenic, adipogenic, renal, hematopoietic or neurogenic differentiation (in 't Anker et al, 2003;Prusa et al, 2004;Tsai et al, 2004Tsai et al, , 2006Karlmark et al, 2005;Bossolasco et al, 2006;Kim et al, 2007;Kolambkar et al, 2007;Perin et al, 2007;Ditadi et al, 2009). Importantly, using immunoselected CD117 (c-Kit)-positive hAFSCs it has been shown that, descending from a single cell, differentiation along adipogenic, osteogenic, myogenic, endothelial, neurogenic and hepatic lineages could be induced.…”

Section: Introductionmentioning

confidence: 99%

Embryoid body formation of human amniotic fluid stem cells depends on mTOR

et al. 2009

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Human amniotic fluid stem cells (hAFSCs) harbor high proliferative capacity and high differentiation potential and do not raise the ethical concerns associated with human embryonic stem cells. The formation of threedimensional aggregates known as embryoid bodies (EBs) is the principal step in the differentiation of pluripotent embryonic stem cells. Using c-Kit-positive hAFSC lines, we show here that these stem cells harbor the potential to form EBs. As part of the two kinase complexes, mTORC1 and mTORC2, mammalian target of rapamycin (mTOR) is the key component of an important signaling pathway, which is involved in the regulation of cell proliferation, growth, tumor development and differentiation. Blocking intracellular mTOR activity through the inhibitor rapamycin or through specific small interfering RNA approaches revealed hAFSC EB formation to depend on mTORC1 and mTORC2. These findings demonstrate hAFSCs to be a new and powerful biological system to recapitulate the three-dimensional and tissue level contexts of in vivo development and identify the mTOR pathway to be essential for this process.

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Neurogenic cells in human amniotic fluid

Cited by 129 publications

References 13 publications

Development of cloned embryos from porcine neural stem cells and amniotic fluid-derived stem cells

Development of cloned embryos from porcine neural stem cells and amniotic fluid-derived stem cells

Molecular and phenotypic characterization of human amniotic fluid cells and their differentiation potential

Embryoid body formation of human amniotic fluid stem cells depends on mTOR

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