Neuroglobin expression in the cochlea of rat pups exposed to chronic very mild carbon monoxide (25 ppm) in air during and after the prenatal period

López, Iván; Acuña, Dora; Shahram, Yalda; Mowlds, Donald; Ngan, Amy M.; Rungvivatjarus, Tiranun; Sharma, Yeshika; Edmond, John

doi:10.1016/j.brainres.2010.02.078

Cited by 10 publications

(16 citation statements)

References 43 publications

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“…The comparison between the immunohistochemical and ISH-results revealed substantial agreement between the Ngb-immunostaining and mRNA-expression patterns in the superior olivary complex of the brainstem (this report), in spiral ganglion neurons in the present and in an earlier study [51] as well as in Scarpa’s ganglion neurons (this report). However, the presence of Ngb protein in the absence of antisense mRNA-Ngb-hybridization in stria vascularis and the basilar membrane suggests that Ngb either is transcribed in these tissues at levels below the sensitivity of our ISH assays or it is transported to these sites after translation.…”

Section: Discussionsupporting

confidence: 87%

“…Besides one report that Ngb is expressed by spiral ganglion neurons [51], detailed information about the distribution of Ngb in mammalian peripheral and central auditory system is lacking. We, therefore, studied the cochlea of rats and mice, and the auditory brainstem of rats, mice and man, using Ngb-immunohistochemistry, in-situ-hybridization, Western blotting, quantitative rt-RT-PCR, and neuronal tract tracing to characterize Ngb-expressing anatomical regions and neuronal cell types.…”

Section: Introductionmentioning

confidence: 99%

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Neuroglobin Expression in the Mammalian Auditory System

et al. 2015

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The energy-yielding pathways that provide the large amounts of metabolic energy required by inner ear sensorineural cells are poorly understood. Neuroglobin (Ngb) is a neuron-specific hemoprotein of the globin family, which is suggested to be involved in oxidative energy metabolism. Here we present quantitative real-time reverse transcription PCR, in situ hybridization, immunohistochemical and Western blot evidence that neuroglobin is highly expressed in the mouse and rat cochlea. For primary cochlea neurons, Ngb expression is limited to the subpopulation of type I spiral ganglion cells, those which innervate inner hair cells, while the subpopulation of type II spiral ganglion cells which innervate the outer hair cells do not express Ngb. We further investigated Ngb distribution in rat, mouse and human auditory brainstem centers, and found that the cochlear nuclei and superior olivary complex (SOC) also express considerable amounts of Ngb. Notably, the majority of olivocochlear neurons, those which provide efferent innervation of outer hair cells as identified by neuronal tract tracing, were Ngb-immunoreactive. We also observed that neuroglobin in the SOC frequently co-localized with neuronal nitric oxide synthase, the enzyme responsible for nitric oxide production. Our findings suggest that neuroglobin is well positioned to play an important physiologic role in the oxygen homeostasis of the peripheral and central auditory nervous system, and provides the first evidence that Ngb signal differentiates the central projections of the inner and outer hair cells.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Neuroglobin Expression in the Mammalian Auditory System

et al. 2015

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“…Neuroglobin immunoreactivity and mRNA expression was described in the rat cochlea (Lopez et al, 2010; Reuss et al, 2015) and cerebella (Beltran-Parrazal et al, 2010). In the rat cochlea Ngb, was localized in the spiral ganglia neurons (SGNs), supporting cells of the organ of Corti, and fibrocytes of the spiral ligament (Lopez et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In the rat cochlea Ngb, was localized in the spiral ganglia neurons (SGNs), supporting cells of the organ of Corti, and fibrocytes of the spiral ligament (Lopez et al, 2010). Ngb expression (mRNA and protein) was decreased in the rat SGNs after mild carbon monoxide exposure, suggesting an association between Ngb and oxidative stress (Lopez et al, 2010). Using immunohistochemistry, in situ hybridization and quantitative real time PCR, Reuss et al, (2015) recently described the expression of Ngb in the rat and mouse peripheral (cochlea) and central auditory system, and in the human superior olivary complex.…”

Section: Introductionmentioning

confidence: 99%

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Neuroglobin immunoreactivity in the human cochlea

Vorasubin

Hosokawa

et al. 2016

Brain Research

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Neuroglobin (Ngb) is an oxygen-binding protein with a demonstrated role in endogenous neuroprotective mechanisms. It has been shown to function as a scavenger for reactive oxidizing species thereby assisting in cellular defense against oxidative stress. In the present study, we characterized the presence of Ngb in the human cochlea. Immunohistochemical staining was performed on formalin fixed celloidin human cochlea sections obtained from human temporal bones, using affinity purified polyclonal antibodies against Ngb. Thirty-six temporal bones were analyzed, 15 with normal otologic histories and 21 diagnosed with different inner ear pathologies. Ngb immunoreactivity (Ngb-IR) was consistently expressed in the neurons of spiral ganglia (SG) and supporting cells of the organ of Corti. There was a significant decrease of Ngb-IR in SGNs from specimens with inner ear pathologies when compared to normal specimens. In contrast, Ngb-IR in the organ of Corti did not show significant changes between pathological and normal specimens. The differential pattern of Ngb expression in these cochlear structures suggests that Ngb may participate in defense mechanisms in inner ear pathologies where oxidative stress is involved.

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Modulatory Effects of Mild Carbon Monoxide Exposure in the Developing Mouse Cochlea

2016

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Carbon monoxide (CO) is well known as a highly toxic poison at high concentrations, yet in physiologic amounts it is an endogenous biological messenger in organs such as the internal ear and brain. In this study we tested the hypothesis that chronic very mild CO exposure at concentrations 25-ppm increases the expression of oxidative stress protecting enzymes within the cellular milieu of the developing inner ear (cochlea) of the normal CD-1 mouse. In addition we tested also the hypothesis that CO can decrease the pre-existing condition of oxidative stress in the mouse model for the human medical condition systemic lupus erythematosus by increasing two protective enzymes heme-oxygenase-1 (HO-1), and superoxide dismutase-2 (SOD-2). CD-1 and MRL/lpr mice were exposed to mild CO concentrations (25 ppm in air) from prenatal only and prenatal followed by early postnatal day 5 to postnatal day 20. The expression of cell markers specific for oxidative stress, and related neural/endothelial markers were investigated at the level of the gene products by immunohistochemistry, proteomics and mRNA expression (quantitative real time-PCR). We found that in the CD-1 and MRL/lpr mouse cochlea SOD-2 and HO-1 were upregulated. In this mouse model of autoimmune disease defense mechanism are attenuated, thus mild CO exposure is beneficial. Several genes (mRNA) and proteins detected by proteomics involved in cellular protection were upregulated in the CO exposed CD-1 mouse and the MRL/lpr mouse.

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Neuroglobin expression in the cochlea of rat pups exposed to chronic very mild carbon monoxide (25 ppm) in air during and after the prenatal period

Cited by 10 publications

References 43 publications

Neuroglobin Expression in the Mammalian Auditory System

Neuroglobin Expression in the Mammalian Auditory System

Neuroglobin immunoreactivity in the human cochlea

Modulatory Effects of Mild Carbon Monoxide Exposure in the Developing Mouse Cochlea

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